中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2008年
5期
599-602
,共4页
脑缺血%再灌注%热休克蛋白质类
腦缺血%再灌註%熱休剋蛋白質類
뇌결혈%재관주%열휴극단백질류
Brain ischemia%Reperfusion%Heat-shock proteins
目的 研究沙鼠脑组织热休克蛋白27(HSP27)的表达及血清抗体含量在脑缺血再灌注后的变化.方法 采用夹闭双侧颈总动脉的方法制作沙鼠脑缺血再灌注模型;60只沙鼠随机分为正常对照组、假手术组、缺血再灌注组、分别在缺血再灌注后(IR)6 h、1、3、7 d的各时间点分批处死动物,双抗夹心法(ELISA)检测血清抗体量,采用免疫组织化学方法检测各组沙鼠脑内HSP27的表达情况,采用SPSS11.5软件进行统计分析.结果 沙鼠缺血再灌注后,脑组织石蜡切片HE染色光镜观察,随缺血时间增加,神经元变性、坏死加重,胶质细胞增生明显,呈现损伤加重倾向.免疫组化检测正常对照组HSP27的阳性细胞为1.52±0.23,假手术组沙鼠脑组织中为1.74 ±0.24,2组比较差异无统计学意义(P＞0.25),阳性细胞主要位于胶质细胞胞浆和神经元中.缺血10 min分别再灌注6 h、1、3、7 d后脑组织中HSP27阳性细胞逐渐增加,在3 d时最多(35.12±2.39),与正常对照组及假手术组比较.差异有统计学意义(P＜0.01);双抗夹心法检测血清HSP27量:假手术组在在6 h,1,3,7 d后抗体量变化很小,与正常对照组比较差异无统计学意义(P＞0.20);缺血后再灌注组在6H,1D,3D,7D后,抗体量先逐渐上升,然后慢慢下降.结论 沙鼠脑组织HSP27在脑缺血再灌注后表达较正常组、假手术组表达升高,提示HSP27表达的增加可能对机体起一定的保护作用.沙鼠血清HSP27抗体量在在脑缺血再灌注后表达较正常组、假手术组表达也升高,表明HSP27血清抗体可能对机体有损伤作用.
目的 研究沙鼠腦組織熱休剋蛋白27(HSP27)的錶達及血清抗體含量在腦缺血再灌註後的變化.方法 採用夾閉雙側頸總動脈的方法製作沙鼠腦缺血再灌註模型;60隻沙鼠隨機分為正常對照組、假手術組、缺血再灌註組、分彆在缺血再灌註後(IR)6 h、1、3、7 d的各時間點分批處死動物,雙抗夾心法(ELISA)檢測血清抗體量,採用免疫組織化學方法檢測各組沙鼠腦內HSP27的錶達情況,採用SPSS11.5軟件進行統計分析.結果 沙鼠缺血再灌註後,腦組織石蠟切片HE染色光鏡觀察,隨缺血時間增加,神經元變性、壞死加重,膠質細胞增生明顯,呈現損傷加重傾嚮.免疫組化檢測正常對照組HSP27的暘性細胞為1.52±0.23,假手術組沙鼠腦組織中為1.74 ±0.24,2組比較差異無統計學意義(P＞0.25),暘性細胞主要位于膠質細胞胞漿和神經元中.缺血10 min分彆再灌註6 h、1、3、7 d後腦組織中HSP27暘性細胞逐漸增加,在3 d時最多(35.12±2.39),與正常對照組及假手術組比較.差異有統計學意義(P＜0.01);雙抗夾心法檢測血清HSP27量:假手術組在在6 h,1,3,7 d後抗體量變化很小,與正常對照組比較差異無統計學意義(P＞0.20);缺血後再灌註組在6H,1D,3D,7D後,抗體量先逐漸上升,然後慢慢下降.結論 沙鼠腦組織HSP27在腦缺血再灌註後錶達較正常組、假手術組錶達升高,提示HSP27錶達的增加可能對機體起一定的保護作用.沙鼠血清HSP27抗體量在在腦缺血再灌註後錶達較正常組、假手術組錶達也升高,錶明HSP27血清抗體可能對機體有損傷作用.
목적 연구사서뇌조직열휴극단백27(HSP27)적표체급혈청항체함량재뇌결혈재관주후적변화.방법 채용협폐쌍측경총동맥적방법제작사서뇌결혈재관주모형;60지사서수궤분위정상대조조、가수술조、결혈재관주조、분별재결혈재관주후(IR)6 h、1、3、7 d적각시간점분비처사동물,쌍항협심법(ELISA)검측혈청항체량,채용면역조직화학방법검측각조사서뇌내HSP27적표체정황,채용SPSS11.5연건진행통계분석.결과 사서결혈재관주후,뇌조직석사절편HE염색광경관찰,수결혈시간증가,신경원변성、배사가중,효질세포증생명현,정현손상가중경향.면역조화검측정상대조조HSP27적양성세포위1.52±0.23,가수술조사서뇌조직중위1.74 ±0.24,2조비교차이무통계학의의(P＞0.25),양성세포주요위우효질세포포장화신경원중.결혈10 min분별재관주6 h、1、3、7 d후뇌조직중HSP27양성세포축점증가,재3 d시최다(35.12±2.39),여정상대조조급가수술조비교.차이유통계학의의(P＜0.01);쌍항협심법검측혈청HSP27량:가수술조재재6 h,1,3,7 d후항체량변화흔소,여정상대조조비교차이무통계학의의(P＞0.20);결혈후재관주조재6H,1D,3D,7D후,항체량선축점상승,연후만만하강.결론 사서뇌조직HSP27재뇌결혈재관주후표체교정상조、가수술조표체승고,제시HSP27표체적증가가능대궤체기일정적보호작용.사서혈청HSP27항체량재재뇌결혈재관주후표체교정상조、가수술조표체야승고,표명HSP27혈청항체가능대궤체유손상작용.
Objective To study the expressions of heat shock protein 27(HSP27)in brain tissue and the changes of antibody of HSP27 in serum after transient cerebral ischemic reperfusion in gerbils.Methods A cerebral ischemia-reperfusion model in gerbils was established by clamping both common carotids.Sixty gerbils were randomly divided into normal control group,sham operation group and ischemia-reperfusion(IR)group.The expressions of HSP27 in brain tissue were detected by immunohistochemistry technique and the changes of antibody of HSP27 were detected by ELISA technique at the 6th hour,1st day,3rd and 7th day after IR.Experimental results were analyzed with the statistic soft package of Spss11.5.Results Experimental results revealed that HSP27 were expressed in neurons and gliacytes of the brain tissue in the 60 gerbils.There was mild expressions of HSP27 in neurons and gliacytes in normal control group and sham operation group, but it has no significant difference between the two groups(P＞0.05).Compared with normal control group,the expressions of HSP27 in neurong and gliacytes of ischemia-reperfusion gerbils were evidently increased at the 6th hour,1st day,3rd day and 7th day,especially at the 3rd day(P＜0.01).Conclusions Expressions of HSP27 in neurons and gliacytes of gerbils were increased markedly.This suggested that the increased expressions of HSP27 could protect the brain tissue from damage.The changes of antibody in serum of normal control group,sham operation group were evidently less than that in group IR,which suggest the HSP27 antibody may damage our body.
