中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2010年
1期
8-10
,共3页
苯%小鼠%红细胞微核
苯%小鼠%紅細胞微覈
분%소서%홍세포미핵
Benzene%Mice%Ecthrocyte micronucleus
目的 探讨苯的不同给药浓度对骨髓红细胞微核改变的影响,拟建立一种理想的苯中毒模型,为进一步苯的实验研究奠定基础.方法 昆明种小鼠50只,随机分为5组:1个对照组,4个实验组,每组10只.对照组单纯皮下注射生理盐水,实验组在每周一、三、五皮下注射苯制剂,并按所给苯剂量分为B1组(0.5 ml/kg体重)、B2组(1.0 ml/kg体重)、B3组(1.5 ml/kg体重)、B4组(2.0 ml/kg体重),各实验组分别于给苯前及给苯1、2.3、4周后称重动物,检测外周血及骨髓中红细胞微核的变化等指标.结果 ①与对照组相比,实验组小鼠白细胞、血红蛋白、血小板三项指标B1、B2组只部分指标改变,B3、B4组在给苯4周后全部指标与对照组相比差异有统计学意义(P均<0.05).②实验组小鼠外周血及骨髓红细胞微核率随着苯注射时间的延长呈现一定的上升趋势,在第3周出现高峰后,第4周下降.③第4周各实验组与对照组比较,小鼠骨髓嗜多染红细胞微核率明显上升,且差异有统计学意义(P均<0.05).结论 苯中毒可以导致小鼠外周血成熟红细胞和骨髓嗜多染红细胞微核率的增高,以此可作为检测骨髓红细胞受抑状态.
目的 探討苯的不同給藥濃度對骨髓紅細胞微覈改變的影響,擬建立一種理想的苯中毒模型,為進一步苯的實驗研究奠定基礎.方法 昆明種小鼠50隻,隨機分為5組:1箇對照組,4箇實驗組,每組10隻.對照組單純皮下註射生理鹽水,實驗組在每週一、三、五皮下註射苯製劑,併按所給苯劑量分為B1組(0.5 ml/kg體重)、B2組(1.0 ml/kg體重)、B3組(1.5 ml/kg體重)、B4組(2.0 ml/kg體重),各實驗組分彆于給苯前及給苯1、2.3、4週後稱重動物,檢測外週血及骨髓中紅細胞微覈的變化等指標.結果 ①與對照組相比,實驗組小鼠白細胞、血紅蛋白、血小闆三項指標B1、B2組隻部分指標改變,B3、B4組在給苯4週後全部指標與對照組相比差異有統計學意義(P均<0.05).②實驗組小鼠外週血及骨髓紅細胞微覈率隨著苯註射時間的延長呈現一定的上升趨勢,在第3週齣現高峰後,第4週下降.③第4週各實驗組與對照組比較,小鼠骨髓嗜多染紅細胞微覈率明顯上升,且差異有統計學意義(P均<0.05).結論 苯中毒可以導緻小鼠外週血成熟紅細胞和骨髓嗜多染紅細胞微覈率的增高,以此可作為檢測骨髓紅細胞受抑狀態.
목적 탐토분적불동급약농도대골수홍세포미핵개변적영향,의건립일충이상적분중독모형,위진일보분적실험연구전정기출.방법 곤명충소서50지,수궤분위5조:1개대조조,4개실험조,매조10지.대조조단순피하주사생리염수,실험조재매주일、삼、오피하주사분제제,병안소급분제량분위B1조(0.5 ml/kg체중)、B2조(1.0 ml/kg체중)、B3조(1.5 ml/kg체중)、B4조(2.0 ml/kg체중),각실험조분별우급분전급급분1、2.3、4주후칭중동물,검측외주혈급골수중홍세포미핵적변화등지표.결과 ①여대조조상비,실험조소서백세포、혈홍단백、혈소판삼항지표B1、B2조지부분지표개변,B3、B4조재급분4주후전부지표여대조조상비차이유통계학의의(P균<0.05).②실험조소서외주혈급골수홍세포미핵솔수착분주사시간적연장정현일정적상승추세,재제3주출현고봉후,제4주하강.③제4주각실험조여대조조비교,소서골수기다염홍세포미핵솔명현상승,차차이유통계학의의(P균<0.05).결론 분중독가이도치소서외주혈성숙홍세포화골수기다염홍세포미핵솔적증고,이차가작위검측골수홍세포수억상태.
Objective To investigate the effect of experimental benzolism on marrow eryghrocyte micromucleus.Methods Fifty male KunMingmice(KM)were randomly divided into 5 groups:one control group and four experimental groups with 10 mice in each group.The mice were injected subcutaneously with sodium chloride in control group and benzene in experimental groups on every Monday,Wednesday and Friday for four weeks.Four experimental groups were injected with a dosage of 0.5 ml/kg(group B1),1.0 nd/kg(group B2),1.5 ml/kg(group B3) and 2.0 ml/ks(group B4),respectively.Before and after injection of sodium chloride or benzene weekly.weight was measured,and the alteration of erythrocyte micronucleus in peripheral blood and marrow were detected.Results ① Compared to the control group,several indices were partly changed in B1 and B2 groups,while all indices changed significantly in group B3 and B4 groups four weeks after injection(P<0.01).②The erythrocyte micronucleus rate of experimental mice in peripheral blood and marrow showed ascending tendency with the extension of benzene injection phase,peak emerged in the third week and decreased in the fourth week.③In the 4th week,compared to the control group,the alteration of erythrocyte micronucleus in marrow in all experimental groups were significantly increaged(P<0.05).Conclusions Benzolism may induce the increasing of micronucleus rate of mature erythrocyte in peripheral blood and erythrocyte in marrow.which can be used to detect status of being restrained in marrow erythrocyte series.
