CAJ | 학술논문

目的探讨膀胱癌组织中微小RNA(miRNA)表达在膀胱癌发生发展中的作用. 方法 Trizol法抽提9例膀胱癌患者癌组织及癌旁正常膀胱组织标本总RNA,分离与富集miRNA,随机选取2组miRNA标本与miRNA表达谱芯片杂交,采用LuxScan 3.0和SAM 2.1软件对芯片结果进行图像数据分析.在差异性表达的miRNA中筛选出感兴趣的let-7基因,采用印迹杂交技术对9组miRNA标本中let-7基因进行验证. 结果膀胱癌组织和正常膀胱组织标本中差异性表达有显著性意义的miRNA 71个,其中表达上调33个,表达下调38个;差异性表达有极显著性意义的miRNA(上调或下调≥4倍)26个,其中显著上调12个,显著下调14个.let-7基因验证结果与芯片表达结果一致,癌组织let-7基因表达吸光度值为479.6±228.2,正常膀胱组织为694.6±152.9,组间差异有统计学意义(P<0.05). 结论膀胱癌组织和正常膀胱组织中存在差异表达的miRNA,let-7基因在膀胱癌的发生中可能起着抑癌基因的作用.
목적 탐토방광암조직중미소RNA(miRNA)표체재방광암발생발전중적작용. 방법 Trizol법추제9례방광암환자암조직급암방정상방광조직표본총RNA,분리여부집miRNA,수궤선취2조miRNA표본여miRNA표체보심편잡교,채용LuxScan 3.0화SAM 2.1연건대심편결과 진행도상수거분석.재차이성표체적miRNA중사선출감흥취적let-7기인,채용인적잡교기술대9조miRNA표본중let-7기인진행험증. 결과 방광암조직화정상방광조직표본중차이성표체유현저성의의적miRNA 71개,기중표체상조33개,표체하조38개;차이성표체유겁현저성의의적miRNA(상조혹하조≥4배)26개,기중현저상조12개,현저하조14개.let-7기인험증결과 여심편표체결과 일치,암조직let-7기인표체흡광도치위479.6±228.2,정상방광조직위694.6±152.9,조간차이유통계학의의(P<0.05). 결론 방광암조직화정상방광조직중존재차이표체적miRNA,let-7기인재방광암적발생중가능기착억암기인적작용.
Objective To detect the differentially expressed microRNAs (miRNAs) in bladder cancer tissue and normal bladder tissue. Methods Total RNA was extracted from bladder cancer tissue and normal bladder tissue by Trizol, and then miRNAs were isolated and enriched from the total RNA. Mammalian miRNA microarrays were used to analyze the differentially expressed miRNAs between the bladder cancer tissue and normal tissue. Data analysis was performed by software of LuxS-can3. 0 and SAM version 2. 1. Choose let-7 gene which was interesting to us, and validation of mi-croarray results was carried out by northern blotting. Results Compared with normal bladder tissues, there were 71 differentially expressed miRNAs in bladder cancer tissue, of which there were 38 down-regulated ones and 33 up-regulated ones. Among these miRNAs, 26 miRNAs were the most significant with 12 up-regulated and 14 down-regulated. The expression of let-7 gene in bladder cancer was down-regulated to normal bladder tissue by northern blotting, which was in agreement with the results of the miRNA microarrays. Conclusion There are differentially expressed miRNAs between bladder cancer tissue and normal bladder tissue, and let-7 gene is probably as a tumor suppressor in bladder cancer.