CAJ | 학술논문

目的研究血管生成促进剂SDY-08对组织修复的影响. 方法 MTT法检查SDY-08对ECV-304细胞生长的影响;鸡胚绒毛尿囊膜(CAM)实验检查SDY-08对CAM血管生成的影响,小鼠背部创伤模型检查SDY-08对组织修复的影响;免疫组化法和Western blotting检查SDY-08对血管内皮细胞生长因子(VEGF)、Bcl-2和Bax表达的影响. 结果 SDY-08(终浓度80 μg/m1)作用ECV-304细胞12,24,36 h,其生长促进率为28.1%,115.6%、81.4%;SDY-08(浓度1.6 mg/ml)对CAM血管生成的诱导率为72.1%;SDY-08(浓度0.5 mg/ml)提前2 d使小鼠创面愈合;SDY-08上调创伤组织血管内皮细胞VEGF的表达,上调ECV-304细胞VEGF和抑凋亡基因Bcl-2的表达,下调促凋亡基因Bax的表达. 结论 SDY-08有明显的促血管生成和促组织修复作用,与其上调VEGF、Bcl-2的表达及下调Bax的表达密切相关.
목적 연구혈관생성촉진제SDY-08대조직수복적영향. 방법 MTT법검사SDY-08대ECV-304세포생장적영향;계배융모뇨낭막(CAM)실험검사SDY-08대CAM혈관생성적영향,소서배부창상모형검사SDY-08대조직수복적영향;면역조화법화Western blotting검사SDY-08대혈관내피세포생장인자(VEGF)、Bcl-2화Bax표체적영향. 결과 SDY-08(종농도80 μg/m1)작용ECV-304세포12,24,36 h,기생장촉진솔위28.1%,115.6%、81.4%;SDY-08(농도1.6 mg/ml)대CAM혈관생성적유도솔위72.1%;SDY-08(농도0.5 mg/ml)제전2 d사소서창면유합;SDY-08상조창상조직혈관내피세포VEGF적표체,상조ECV-304세포VEGF화억조망기인Bcl-2적표체,하조촉조망기인Bax적표체. 결론 SDY-08유명현적촉혈관생성화촉조직수복작용,여기상조VEGF、Bcl-2적표체급하조Bax적표체밀절상관.
Objective To investigate the effects of SDY-08 isolated from Dasyatis akajei on tis-sue repair. Methods MTT assay was performed to measure the effect of SDY-08 on the growth of ECV-304 cells. The effect of SDY-08 on angiogenesis was detected in the chick embryochorioallantoic membrane (CAM). Mouse wound model was applied to investigate the effect of SDY-08 on tissue repair. Immunohistochemical staining assay and Western blotting were adopted to examine the expression changes of vascular endothelial growth factor (VEGF), Bcl-2 and Bax in wound tissues in response to SDY-08. Results The proliferation rates of SDY-08 at final concentration of 80 μg/ml promoting ECV-304 cells were 28.1%, 115.6% and 81.4% respectively at 12, 24 and 36 hours. The induction rate of angiogene-sis of CAM by SDY-08 at concentration of 1.6 mg/ml was 72.1%. SDY-08 at 0.5 mg/ml markedly in-duced acceleration of wound healing in mouse model two days in advance. SDY-08 up-regulated either ex-pressions of VEGF in trauma group or that of Bcl-2 and VEGF of ECV-304 cells, but down-regulated ex-pression of Box. Conclusions SDY-08 can significantly promote angiogenesis and tissue repair, which is closely correlated with its effect of up-regulating expressions of VEGF and Bcl-2 as well with that of down-regulating expression of Box.