CAJ | 학술논문

目的研究汉防己甲素(Tet)和屈洛昔芬(DRL)单独及联合用药后对K562细胞及其耐药细胞株K562/A02的核因子κB(NF-κB)表达的影响,以进一步探讨其逆转多药耐药的作用机制.方法采用免疫细胞化学及Western blotting法分别检测Tet(1μmol/L)、DRL(5 μmol/L)单独及联合作用于K562和K562/A02细胞后NF-κB核转位水平和胞核NF-κB蛋白表达水平的变化.结果①K562/A02细胞NF-κB核转位水平[(65.0±4.2)%]及胞核NF-κB蛋白表达(3.545±0.219)明显高于K562细胞[(39.6±0.9)%和2.366±0.141](P＜0.01);②Tet、DRL单独及联合作用6、12 h,对K562细胞NF-κB核转位水平及胞核NF-κB蛋白表达水平无明显影响(P＞0.05);③两药单独及联合作用6、12 h,可明显降低K562/A02细胞NF-κB蛋白核转位水平和胞核NF-κB蛋白表达水平(P＜0.05和P＜0.01),两药联合作用增强(P＜0.05),作用12 h较作用6 h效果更显著(P＜0.05).结论K562/A02细胞的胞核NF-κB蛋白表达水平明显高于K562细胞,NF-κB的活化可能参与了K562/A02细胞耐药的发生;抑制NF-κB活化可能参与了Tet和DRL逆转K562/A02细胞多药耐药过程.
목적 연구한방기갑소(Tet)화굴락석분(DRL)단독급연합용약후대K562세포급기내약세포주K562/A02적핵인자κB(NF-κB)표체적영향,이진일보탐토기역전다약내약적작용궤제.방법 채용면역세포화학급Western blotting법분별검측Tet(1μmol/L)、DRL(5 μmol/L)단독급연합작용우K562화K562/A02세포후NF-κB핵전위수평화포핵NF-κB단백표체수평적변화.결과①K562/A02세포NF-κB핵전위수평[(65.0±4.2)%]급포핵NF-κB단백표체(3.545±0.219)명현고우K562세포[(39.6±0.9)%화2.366±0.141](P＜0.01);②Tet、DRL단독급연합작용6、12 h,대K562세포NF-κB핵전위수평급포핵NF-κB단백표체수평무명현영향(P＞0.05);③량약단독급연합작용6、12 h,가명현강저K562/A02세포NF-κB단백핵전위수평화포핵NF-κB단백표체수평(P＜0.05화P＜0.01),량약연합작용증강(P＜0.05),작용12 h교작용6 h효과경현저(P＜0.05).결론K562/A02세포적포핵NF-κB단백표체수평명현고우K562세포,NF-κB적활화가능삼여료K562/A02세포내약적발생;억제NF-κB활화가능삼여료Tet화DRL역전K562/A02세포다약내약과정.
Objective To study the effect of tetrandrine (Tet) in combination with droloxifen(DRL)on the expression of nuclear factor kappa B(NF-κB) in K562 and K562/A02 cell lines and its reversal mechanism. Methods The activation of NF-κB in K562 and K562/A02 cell lines and the effect of Tet or DRL alone or in combination on NF-κB protein expression were determined with immunocytochemistry and down-regulate NF-κB protein expression in nuclei of K562/A02 cells. The effect was more significant in commay be involved in the reversal of multidrug resistance in K562/A02 cells by Tet and DRL.