CAJ | 학술논문

目的探讨雌激素对慢性高眼压兔视网膜损伤的保护作用.方法 18只成年健康新西兰雌性大白兔随机分为去势组和假去势组.去势组切除两侧卵巢,假去势组仅切除卵巢周围部分脂肪组织.利用前房注射复方卡波姆建立兔慢性高眼压模型.采用末端脱氧核酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法(TUNEL)检测视网膜组织中凋亡细胞的表达;免疫组织化学方法检测B细胞淋巴瘤/白血病(bcl)-2、bax蛋白的表达情况;应用计算机图像分析系统对检测结果进行分析.结果造模后4、6、8周,去势组较假去势组视网膜神经节细胞数目明显减少,视神经纤维层和内核层变薄;TUNEL阳性细胞表达均较假去势组高(t=3.285,4.012,3.624;P值均<0.01);视网膜bcl-2表达均较假去势组低(t=4.256,3.867,3.459;P值均<0.01);bax阳性细胞表达情况均较假去势组高(t=3.211,3.625,3.253;P值均<0.01).bcl-2的表达与TUNEL的表达呈负相关,凋亡细胞随着bcl-2的表达增多而减少,bcl-2抑制了凋亡的发生.bax的表达与TUNEL的表达呈正相关,凋亡细胞随着bax的表达增多而增多,bax诱导了凋亡的发生.结论雌激素对慢性高眼压兔视网膜损伤有神经保护作用,这一保护作用主要是通过减少视网膜组织中的凋亡细胞而实现的.
목적 탐토자격소대만성고안압토시망막손상적보호작용.방법 18지성년건강신서란자성대백토수궤분위거세조화가거세조.거세조절제량측란소,가거세조부절제란소주위부분지방조직.이용전방주사복방잡파모건립토만성고안압모형.채용말단탈양핵산전이매개도적탈양삼린산뇨감결구말단표기법(TUNEL)검측시망막조직중조망세포적표체;면역조직화학방법검측B세포림파류/백혈병(bcl)-2、bax단백적표체정황;응용계산궤도상분석계통대검측결과진행분석.결과 조모후4、6、8주,거세조교가거세조시망막신경절세포수목명현감소,시신경섬유층화내핵층변박;TUNEL양성세포표체균교가거세조고(t=3.285,4.012,3.624;P치균<0.01);시망막bcl-2표체균교가거세조저(t=4.256,3.867,3.459;P치균<0.01);bax양성세포표체정황균교가거세조고(t=3.211,3.625,3.253;P치균<0.01).bcl-2적표체여TUNEL적표체정부상관,조망세포수착bcl-2적표체증다이감소,bcl-2억제료조망적발생.bax적표체여TUNEL적표체정정상관,조망세포수착bax적표체증다이증다,bax유도료조망적발생.결론 자격소대만성고안압토시망막손상유신경보호작용,저일보호작용주요시통과감소시망막조직중적조망세포이실현적.
Objective To investigate the protective effects of estrogen on rabbit retinal damages induced by chronic ocular hypertension. Methods A total of 18 white New Zealand female rabbits were randomly divided into ovariectomized (OV) group and sham OV (SOV) group. Bilateral ovaries were remove in OV group while only the adipose tissue around ovarian were remove in SOV group. Chronic ocular hypertension was induced by anterior chamber injection of carbomer. Retinal cell apoptosis was measured by terminal deoxynucteotidyl transferase-mediated dUTP nick end labeling (TUNEL), the expression of bcl-2,bax were detected by immunohistochemistry. The images were captured under microscope and analyzed with computer-image-analysis system. Results Four, six and eight weeks after ocular hypertension modeling,the OV retinas have less retinal ganglion cells, thinner optic nerve fiber layer and inner nuclear layer and more TUNEL positive cells (t = 3. 285, 4. 012, 3. 624; P< 0.01). The OV retinas also have less bcl-2 expression (t=4. 256,3. 867,3. 459;P<0. 01), more bax positive cells (t=3. 211,3. 625,3. 253;P<0. 01).Bcl-2 expression was negatively correlated with TUNEL positive cells indicating bcl-2 can inhibit apoptosis.Bax expression was positively correlated with TUNEL positive cells indicating bax induce apoptosis.Conclusion Estrogen has a neuroprotection role to rabbit retina under chronic ocular hypertension by reducing apoptosis.