CAJ | 학술논문

目的建立6种川产姜黄属(Curcuma)药用植物快速简单的分子鉴定方法.方法采用叶绿体赖氨酸tRNA基因(trnK)测序与序列变异分析方法.结果 6种姜黄属药用植物(包括姜黄C. longa、莪术C. phaeocaulis、川郁金C. sichuanensis、川郁金C. chuanyujin、川黄姜C. chuanhuangjiang、川莪术C. chuanezhu)完整trnK基因长度在2699～2705 bp.序列可变区包括matK基因编码区和trnK外显子与matK内含子之间区域,共有6个单核苷酸多态性(SNPs)位点、1个9-bp的缺失重复序列和2个4-bp、14-bp插入重复序列.结论 trnK基因序列可变位点可以作为6种川产姜黄属药用植物快速简单的分子鉴定标记,并为它们之间种的归并提供了分子依据.
목적건립6충천산강황속(Curcuma)약용식물쾌속간단적분자감정방법.방법채용협록체뢰안산tRNA기인(trnK)측서여서렬변이분석방법.결과 6충강황속약용식물(포괄강황C. longa、아술C. phaeocaulis、천욱금C. sichuanensis、천욱금C. chuanyujin、천황강C. chuanhuangjiang、천아술C. chuanezhu)완정trnK기인장도재2699～2705 bp.서렬가변구포괄matK기인편마구화trnK외현자여matK내함자지간구역,공유6개단핵감산다태성(SNPs)위점、1개9-bp적결실중복서렬화2개4-bp、14-bp삽입중복서렬.결론 trnK기인서렬가변위점가이작위6충천산강황속약용식물쾌속간단적분자감정표기,병위타문지간충적귀병제공료분자의거.
Aim To establish a rapid and simple molecular identification method for six medicinals: Curcuma: C. longa, C. phaeocaulis, C. sichuanensis, C. chuanyujin, C. chuanhuangjiang, and C. chuanezhu in Sichuan Province. Methods A molecular approach (trnK nucleotide sequencing) was used in this study. Results The sequenced entire chloroplast trnK gene region spanned 2 699-2 705 bp. The matK gene (an intron embodied in trnK gene) sequence and the intron spacer region of the trnK gene have great diversity within these six medicinal Curcuma species. There were six single bases substitutions between trnK coding region and matK region, the 9-bp deletion and 4-bp or 14-bp insertion repeat at some sites of matK region in each taxon. Conclusion These relatively variable sequences were potentially informative in the identification for these six Curcuma species at the DNA level.