CAJ | 학술논문

研究了光敏核不育水稻(Oryza sativa L.)农垦58S(NK58S)的光合日变化和光抑制.06:00～09:00,NK58S的光抑制不明显,此时的光合功能下调以叶黄素循环为主;10:00～12:00,耗散比能流(DIo/RC)及光反应中心关闭净速率(dV/dto)增加,受体侧电子传递受阻(ψo下降),活性反应中心密度(Do)降低,NK58S光抑制加剧,PSⅡ反应中心发生失活.荧光暗弛豫分析与抑制剂处理结果表明,状态转换、叶黄素循环和PSⅡ反应中心失活均能有效保护NK58S免遭强光损伤.叶黄素循环相对于反应中心失活,前者是NK58S对强光胁迫的快速反应,在光强相对较弱时发挥主要作用,而后者在叶黄素循环达到饱和时对保护剩余活性反应中心起主要作用.
연구료광민핵불육수도(Oryza sativa L.)농은58S(NK58S)적광합일변화화광억제.06:00～09:00,NK58S적광억제불명현,차시적광합공능하조이협황소순배위주;10:00～12:00,모산비능류(DIo/RC)급광반응중심관폐정속솔(dV/dto)증가,수체측전자전체수조(ψo하강),활성반응중심밀도(Do)강저,NK58S광억제가극,PSⅡ반응중심발생실활.형광암이예분석여억제제처리결과표명,상태전환、협황소순배화PSⅡ반응중심실활균능유효보호NK58S면조강광손상.협황소순배상대우반응중심실활,전자시NK58S대강광협박적쾌속반응,재광강상대교약시발휘주요작용,이후자재협황소순배체도포화시대보호잉여활성반응중심기주요작용.
The diurnal photosynthesis and photoinhibition in the photoperiod-sensitive genic male-sterile rice (Oryza sativa L.), Nongken 58S (NK58S), were investigated. From 06:00 to 09:00, no remarkable photoinhibition occurred, and the down-regulation of photosynthesis might be due to the running of xanthophylls cycle. From 10:00 to 12:00, the specific energy flux for dissipation (DIo/RC) and the net rate of reaction centers (RCs) closure (dV/dto) were increased, while the probability of electron transport at the acceptor side (ψo) and the density of active RCs (Do) were decreased. These indicated that the photoinhibition of NK58S was exacerbated with the inactivation of PS Ⅱ RCs. Fluorescence dark relaxation analysis and inhibitor treatment suggested that all of state transition, xanthophyll cycle and inactivation of PS Ⅱ RCs could contribute to protect NK58S against photodamage. Compared with the inactivation of PS Ⅱ RCs, xanthophyll cycle had an immediate response to high light stress, which functioned mainly in the period of relatively low light intensity. However, the inactivation of PS Ⅱ RCs played an important role in protecting the remaining active RCs when xanthophyll cycle was saturated.