CAJ | 학술논문

目的制备胰岛素单克隆抗体,用此抗体检测2型糖尿病患者血清胰岛素水平.方法以交联胰岛素-牛血清清蛋白为免疫原,免疫Balb/c小鼠,用杂交瘤技术制备得到4株稳定分泌胰岛素抗体的单克隆细胞株,对单抗效价、亚类、亲和常数进行了分析.利用所得单抗,通过竞争性抑制酶联免疫吸附法(ELISA)随机检测90例2型糖尿病患者血清和90例正常人血清中胰岛素的含量.结果制备的单抗亲和常数达1011,除1株属IgG26(к)外,其余3株为IgG1(к),检测发现2型糖尿病患者血清胰岛素含量低于正常人水平.结论 2型糖尿病患者血清胰岛素平均低于正常人水平,可能由于胰腺功能衰竭所致,仍可用补充胰岛素缓解病情.约10%患者血清胰岛素含量高于正常人平均水平,可能由于体内胰岛素拮抗所致,不宜用胰岛素治疗.
목적 제비이도소단극륭항체,용차항체검측2형당뇨병환자혈청이도소수평.방법 이교련이도소-우혈청청단백위면역원,면역Balb/c소서,용잡교류기술제비득도4주은정분비이도소항체적단극륭세포주,대단항효개、아류、친화상수진행료분석.이용소득단항,통과경쟁성억제매련면역흡부법(ELISA)수궤검측90례2형당뇨병환자혈청화90례정상인혈청중이도소적함량.결과 제비적단항친화상수체1011,제1주속IgG26(к)외,기여3주위IgG1(к),검측발현2형당뇨병환자혈청이도소함량저우정상인수평.결론 2형당뇨병환자혈청이도소평균저우정상인수평,가능유우이선공능쇠갈소치,잉가용보충이도소완해병정.약10%환자혈청이도소함량고우정상인평균수평,가능유우체내이도소길항소치,불의용이도소치료.
Objective The monoclone antibody against insulin was prepared and used to measure plasma level of insulin in patients with type 2 diabetes mellitus. Methods Balb/c mice were immunedwith cross-linking insulin-BSA as immunogen. Four monoelone cell strains stably secreting insulin monoclone antibody were prepared by applying hybridoma technique. The valence, subclass and affini-ty constant of the monoclone antibody were analyzed. The serum insulin content was measured with competitive inhibition ELISA in 90 patients with type 2 diabetes mellitus and 90 healthy controls. Re-suits The affinity constant of the established monoclone antibody reached to 1011. The subclass of 1 strain was Igab(k),and the other 3 strains were Ig1(k) srbclass.The mean level of insulin was significantly lower in patients with type 2 diabetes mellitus than that of healthy controls. Conclusion These results implicated that the insulin insufficient might be due to the depletion of the pancreatic be-ta cell function of patients with type 2 diabetes mellitus, and the pathogenetic condition might be alle-viated by supplying insulin. About 10% patients are with higher insulin level than healthy controls,which may be due to insulin resistance. It is inadvisable to treat these patients with insulin therapy.