CAJ | 학술논문

分别提取'红核子'龙眼(Dimdcarpus longan Lour.)谢花后35 d(早期)和50 d(晚期)子叶胚RNA,建立cDNA-AFLP分析体系,获得指纹图谱.对41个差异片段回收克隆和序列分析,表明其中21个与已知功能基因具有高度同源性,这些基因的功能主要涉及侧牛器官的离轴极性、糖酵解、能最代谢、离子转运、细胞壁伸长、细胞周期调控、RNA转录、RNA翻译和调控、蛋白磷酸化调控和降解、信号转导等;其余片段与已知基因的同源性较低或没有.
분별제취'홍핵자'용안(Dimdcarpus longan Lour.)사화후35 d(조기)화50 d(만기)자협배RNA,건립cDNA-AFLP분석체계,획득지문도보.대41개차이편단회수극륭화서렬분석,표명기중21개여이지공능기인구유고도동원성,저사기인적공능주요섭급측우기관적리축겁성、당효해、능최대사、리자전운、세포벽신장、세포주기조공、RNA전록、RNA번역화조공、단백린산화조공화강해、신호전도등;기여편단여이지기인적동원성교저혹몰유.
The finger map of longan(Dimdcarpus longan Lour.'Honghezi')cotyledon embryo RNA was established by cDNA-AFLP,which extracted from thirty-five-day(early)and fety-day(late)cotyledon embryos after anthesis,respectively.There were 41 differential fragments cloned and sequence analysed,in which 21 differential expression fragments had high homology with some known genes,the function mainly involved in the abaxial polarity of lateral organ,glycolysis,energy metabolism,ion transport,cell wall elongation,cell cycle regulation,RNA transcription,RNA translation and regulation,protein phosphorylation regubtion and protein degradation,signal transduction and so on.While the others had low or no homology with known genes.