CAJ | 학술논문

目的探讨胡椒醇提液外用对兔耳轻度冻伤的治疗效果.方法用-15℃左右低温液复制兔耳轻度冻伤模型,冻伤后48h开始在兔冻耳涂抹胡椒70%乙醇提取液治疗,2 d后测定血清中SOD活性、MDA和TNF-α含量,4 d后评价冻耳外观正常与否及变形程度,制作冻耳组织病理切片,显微镜下观察冻耳组织病理变化,同时测定血清与冻耳组织中SOD活性、MDA和TNF-α含量.结果用胡椒70%乙醇提取液治疗2 d后血清中SOD活性显著增加,MDA量显著降低,TNF-α量无明显变化.治疗4d后血清中SOD活性、MDA和TNF-α含量均无明显差异,但冻耳组织中SOD活性与TNF-α水平显著提高,MDA量显著降低.另外冻耳外观评价与组织病理检查显示冻耳组织坏死范围减少,修复速度明显提高.结论胡椒70%乙醇提取液局部外用对轻度冻伤有治疗作用.
목적 탐토호초순제액외용대토이경도동상적치료효과.방법 용-15℃좌우저온액복제토이경도동상모형,동상후48h개시재토동이도말호초70%을순제취액치료,2 d후측정혈청중SOD활성、MDA화TNF-α함량,4 d후평개동이외관정상여부급변형정도,제작동이조직병리절편,현미경하관찰동이조직병리변화,동시측정혈청여동이조직중SOD활성、MDA화TNF-α함량.결과 용호초70%을순제취액치료2 d후혈청중SOD활성현저증가,MDA량현저강저,TNF-α량무명현변화.치료4d후혈청중SOD활성、MDA화TNF-α함량균무명현차이,단동이조직중SOD활성여TNF-α수평현저제고,MDA량현저강저.령외동이외관평개여조직병리검사현시동이조직배사범위감소,수복속도명현제고.결론 호초70%을순제취액국부외용대경도동상유치료작용.
Objective To investigate the therapeutic effect of alcohol extract from pepper on mild and moderate frostbite of rabbit ear. Methods The right rabbit ear, within 7 cm scope from apex conchae auris toward sulcus auriculae posterior, was immersed into cold liquid at -15℃ to establish an experimental model of mild and moderate frostbite. 2 d after frostbite, 70% alcohol extract from pepper was applied onto surface of ears frozen. SOD activity, MDA and TNF-α levels in serum were determined 2 and 4 d after treatment. Then after application for 4 d, SOD activity, contents of MDA and TNF-α in tissue of ear frozen were detected. In addition, the recovery profiles of ear frozen like appearance variables and histopathologic features were surveyed.Results SOD activity in serum significantly increased 2 d after treatment with 70% alcohol extract from pepper, MDA level significantly decreased and TNF-α level had no difference. SOD activity, MDA and TNF-α concentrations in serum showed no differences after administration for 4 d.In tissue of the affected ear, SOD activity and TNF-α contents were obviously at high levels at this time, MDA content was the opposite. On the other hand, the appearance review and histopathologic examination both manifested that topical application of 70% alcohol extract from pepper diminished necrotic regions of ear frozen, accelerated tissue repair after frostbite. Conclusion 70% alcohol extract of pepper has pharmacological activity on mild and moderate frostbite healing.