CAJ | 학술논문

以三疣梭子蟹莱州湾、舟山野生群体杂交(莱州湾♀×舟山♂)产生F_2代家系63个个体及F1代亲本为实验群体,采用AFLP分子标记对其进行遗传分析.35对选择性引物共扩增出1 742个位点,其中分离位点为417个,不分离位点为1 325个,多态位点比例为23.9%.在分离位点中,符合1:1孟德尔分离比例的位点为226个,占总分离位点数的54.2%;偏离1:1孟德尔分离比例的位点为31个,占总分离位点数7.4%.符合3:1孟德尔分离比例的位点为128个,占总分离位点数的30.7%;偏离3:1孟德尔分离比例的位点为32个,占总分离位点数的7.7%.家系内基因多样性指数为0.130,Shannon指数为0.193.遗传距离分析显示,子代个体与母本Nei's遗传距离介于0.064～0.111之间,与父本Nei's遗传距离介于0.065～0.107之间.其中,63号个体与48号个体分别与父、母本亲缘关系最远.本实验中,偏分离位点比例属正常范围,杂交F_2代家系遗传多样性偏低.本研究结果为三疣梭子蟹野生群体及家系遗传多样性分析,遗传连锁图谱构建等方面提供一定的技术支持.
이삼우사자해래주만、주산야생군체잡교(래주만♀×주산♂)산생F_2대가계63개개체급F1대친본위실험군체,채용AFLP분자표기대기진행유전분석.35대선택성인물공확증출1 742개위점,기중분리위점위417개,불분리위점위1 325개,다태위점비례위23.9%.재분리위점중,부합1:1맹덕이분리비례적위점위226개,점총분리위점수적54.2%;편리1:1맹덕이분리비례적위점위31개,점총분리위점수7.4%.부합3:1맹덕이분리비례적위점위128개,점총분리위점수적30.7%;편리3:1맹덕이분리비례적위점위32개,점총분리위점수적7.7%.가계내기인다양성지수위0.130,Shannon지수위0.193.유전거리분석현시,자대개체여모본Nei's유전거리개우0.064～0.111지간,여부본Nei's유전거리개우0.065～0.107지간.기중,63호개체여48호개체분별여부、모본친연관계최원.본실험중,편분리위점비례속정상범위,잡교F_2대가계유전다양성편저.본연구결과위삼우사자해야생군체급가계유전다양성분석,유전련쇄도보구건등방면제공일정적기술지지.
Portunus trituberculatus is one of the most important fishery resources in China because of its high commercial value. In recent years, it has suffered from overfishing and various diseases, and the total resource of wild populations has decreased year by year. Considering for this situation, it is necessary to study the genetic diversity and genetic linkage map of P. Trituberculatus. In this study, maternal broodstock and paternal broodstock were sampled from Laizhou Bay and Zhoushan Island wild generated F1 family. 63 individuals of F_2 family came from sisterhood intercross between F1 family and these broodstock were used as experiment materials. The AFLP analyses showed that a total of 1 742 loci were produced,of which 417 loci were polymorphic, accounting for 23.9%. In these polymorphic loci, there were 226 (accounting for 54.2% of total segregating loci) loci segregating as 1:1 Mendelian model while 31 (7.4%) loci segregating as distorted model.128 loci (30.7%) segregated as 3:1 model and the numbers of 32 (7.7%) loci were distortion bands. Additionally, several AFLP bands which segregated neither as 1:1 nor as 3:1 were named with odd segregating loci. They were treated as non-segregating loci in order to reduce the influence of statistical error. Genetic diversity index of the F_2was 0.130, and Shannon genetic diversity index was 0.193. Analysis of distances showed that Nei's genetic distance of family with male parent and female parent were between 0.064～0.111,0.065～0.107 respectively. In the mean time, No63 individual was most distant from its male parent and No48 individual was most distant from its female parent. The main purpose of the study was to provide the technical support for genetic diversity analysis of wild populations or families and construction of high density genetic linkage map of P. Trituberculatus.