CAJ | 학술논문

葡萄籽原花青素的相对分子质量分布和平均分子量的测定一直是研究的难点.通过酰基化后用激光光散射与GPC联用方法对葡萄籽原花青素的分子量分布和平均分子量进行测定,结果表明:葡萄籽原花青素提取物的平均相对分子质量为9.39×10~4,相对分子质量分布较宽,多分散系数为1.65;葡萄籽原花青素纯化物1的平均相对分子质量为9.64×10~3,多分散系数为1.56,相对分子质量分布曲线呈对称分布,聚合度13～15,以高聚合度原花青索为主;葡萄籽原花青素纯化物2的平均相对分子质量为4.25×10~3,相对分子质量分布较窄,多分散系数为1.45,相对分子质量分布曲线基本呈对称分布,聚合度6-7,为低聚合度原花青素.
포도자원화청소적상대분자질량분포화평균분자량적측정일직시연구적난점.통과선기화후용격광광산사여GPC련용방법대포도자원화청소적분자량분포화평균분자량진행측정,결과표명:포도자원화청소제취물적평균상대분자질량위9.39×10~4,상대분자질량분포교관,다분산계수위1.65;포도자원화청소순화물1적평균상대분자질량위9.64×10~3,다분산계수위1.56,상대분자질량분포곡선정대칭분포,취합도13～15,이고취합도원화청색위주;포도자원화청소순화물2적평균상대분자질량위4.25×10~3,상대분자질량분포교착,다분산계수위1.45,상대분자질량분포곡선기본정대칭분포,취합도6-7,위저취합도원화청소.
Proanthocyanidin is ubiquitous in nature.however.the methods to determine the relative molecularweight(RMW)and the distribution RMW of grape seed proanthoeyanidin(GSP)have not been well developed.In thisstudy,laser light scattering with GPC Was employed to determine the average RMW and the distribution of RM W of GSP ingrape seed after aeetylate treatment.The results showed that the average RMW of acetylated GSP was 9.39×10~4;it's RMW distributed widely with the polydispersity being 1.65.The average RMW of acetylated GSPPl was 9.64×10~4;it's RMW showed a symmetrical distribution with the polydispersity being 1.56,the polymer degree being 13-15 for the hiighpolymeric proanthoeyanidin.The average RMW of acetylated GSPP2 Was 4.25×10~3;it's RMW exhibite a symmetrical dis.tribution with the polydispersity being 1.56.the polymer degree being 6-7 for the oligomeric proanthocyanidin.