华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2009年
6期
768-770,774
,共4页
李豫峰%田红帅%李舟%刘群%章汉旺%任新玲%魏玉兰%胡娟%朱桂金
李豫峰%田紅帥%李舟%劉群%章漢旺%任新玲%魏玉蘭%鬍娟%硃桂金
리예봉%전홍수%리주%류군%장한왕%임신령%위옥란%호연%주계금
核抑制剂%未成熟卵母细胞%体外成熟%纺锤体%染色体
覈抑製劑%未成熟卵母細胞%體外成熟%紡錘體%染色體
핵억제제%미성숙란모세포%체외성숙%방추체%염색체
Roscovitine%immature oocytes%in vitro maturation%spindle%chromosome
目的 观察核抑制剂(Roscovitine)对人未成熟卵母细胞体外成熟(in vitro maturation,IVM)安全性的影响.方法 获取促排周期的GV期未成熟卵随机分为两组,实验组(经二步法):未成熟卵母细胞先用含有200 vmol/LRoseovitine的培养液培养6 h,然后转入IVM成熟培养液培养24～36 h;对照组:直接用IVM成熟培养液培养24~36h.观察两组卵母细胞成熟情况并应用激光扫描共聚焦显微镜观察培养成熟后MⅡ期卵母细胞纺锤体和染色体的形态.结果 实验组共获未成熟卵母细胞61个,经体外成熟培养后50个卵成熟,其中39个卵可观察到纺锤体与染色体,纺锤体的形态异常者为15个,染色体异常者11个;对照组共获未成熟卵母细胞60个,经体外成熟培养后43个卵成熟,其中32个卵可观察到染色体与纺锤体,纺锤体形态异常者14个,染色体异常者11个.结论 Roscovitine能可逆性抑制体外培养的人卵母细胞的核成熟,但未对纺锤体及染色体形态产生不良影响.
目的 觀察覈抑製劑(Roscovitine)對人未成熟卵母細胞體外成熟(in vitro maturation,IVM)安全性的影響.方法 穫取促排週期的GV期未成熟卵隨機分為兩組,實驗組(經二步法):未成熟卵母細胞先用含有200 vmol/LRoseovitine的培養液培養6 h,然後轉入IVM成熟培養液培養24～36 h;對照組:直接用IVM成熟培養液培養24~36h.觀察兩組卵母細胞成熟情況併應用激光掃描共聚焦顯微鏡觀察培養成熟後MⅡ期卵母細胞紡錘體和染色體的形態.結果 實驗組共穫未成熟卵母細胞61箇,經體外成熟培養後50箇卵成熟,其中39箇卵可觀察到紡錘體與染色體,紡錘體的形態異常者為15箇,染色體異常者11箇;對照組共穫未成熟卵母細胞60箇,經體外成熟培養後43箇卵成熟,其中32箇卵可觀察到染色體與紡錘體,紡錘體形態異常者14箇,染色體異常者11箇.結論 Roscovitine能可逆性抑製體外培養的人卵母細胞的覈成熟,但未對紡錘體及染色體形態產生不良影響.
목적 관찰핵억제제(Roscovitine)대인미성숙란모세포체외성숙(in vitro maturation,IVM)안전성적영향.방법 획취촉배주기적GV기미성숙란수궤분위량조,실험조(경이보법):미성숙란모세포선용함유200 vmol/LRoseovitine적배양액배양6 h,연후전입IVM성숙배양액배양24～36 h;대조조:직접용IVM성숙배양액배양24~36h.관찰량조란모세포성숙정황병응용격광소묘공취초현미경관찰배양성숙후MⅡ기란모세포방추체화염색체적형태.결과 실험조공획미성숙란모세포61개,경체외성숙배양후50개란성숙,기중39개란가관찰도방추체여염색체,방추체적형태이상자위15개,염색체이상자11개;대조조공획미성숙란모세포60개,경체외성숙배양후43개란성숙,기중32개란가관찰도염색체여방추체,방추체형태이상자14개,염색체이상자11개.결론 Roscovitine능가역성억제체외배양적인란모세포적핵성숙,단미대방추체급염색체형태산생불량영향.
Objective To investigate the effects of 200μmol/L nuclear inhibiter(Roscovitine) on maturation and security in vitro in human immature oocytes.Methods Immature oocytes were cultured with a two-step culture system.Firstly immature oocytes were cultured in maturation medium with Roscovitine for 6 h,then in maturation medium without Roscovitine for 24-36 h.Laser confocal scanning microscopy was used to examine the meiotic spindle and chromosome.The oocytes in control group were cultured in TCM-199 maturation medium.Results In experimental group 61 oocytes were observed.After in vitro maturation,50 oocytes became maturation,and 39 of them had spindle and chromosome,including 15 abnormal spindles and 11 abnormal chromosomes.In control group 60 oocytes were observed.After in vitro maturation 43 became maturation,and 32 of them had spindles and chromosomes,including 14 abnormal spindles and 11 abnormal chromosomes.Conclusion Roscovitine can reversibly inhibit nuclear maturation of human oocytes,but has no adverse effects on spindle and chromosome configuration.
