CAJ | 학술논문

目的研究低剂量照射(6 cGy)的骨髓细胞悬液,离心后得到的上层相(刺激液)对正常或辐射损伤细胞能否产生低剂量辐射兴奋效应,并探讨其机制.方法刺激液与受0、2或5 Gy照射的骨髓细胞悬液进行混合培养,使用MTT比色法检测各组细胞的增殖能力,采用细胞色素c还原法测定细胞中O2-的浓度.最后,通过加入二亚苯基碘和肉豆蔻醋酸酯实验性、特异地降低或提高细胞中O2-的浓度,观察此种变化对刺激液产生上述作用的影响.结果正常和受大剂量照射的骨髓细胞与刺激液共培养后,其增殖能力明显高于对照组.减少细胞中O2-的浓度可降低辐射损伤细胞的增殖活力,而增加细胞中O2-的浓度可提高辐射损伤细胞的增殖能力.结论上述低剂量辐射兴奋效应发生的机制可能与细胞中O2-浓度的变化有关.
목적 연구저제량조사(6 cGy)적골수세포현액,리심후득도적상층상(자격액)대정상혹복사손상세포능부산생저제량복사흥강효응,병탐토기궤제.방법 자격액여수0、2혹5 Gy조사적골수세포현액진행혼합배양,사용MTT비색법검측각조세포적증식능력,채용세포색소c환원법측정세포중O2-적농도.최후,통과가입이아분기전화육두구작산지실험성、특이지강저혹제고세포중O2-적농도,관찰차충변화대자격액산생상술작용적영향.결과 정상화수대제량조사적골수세포여자격액공배양후,기증식능력명현고우대조조.감소세포중O2-적농도가강저복사손상세포적증식활력,이증가세포중O2-적농도가제고복사손상세포적증식능력.결론 상술저제량복사흥강효응발생적궤제가능여세포중O2-농도적변화유관.
Objective To investigate whether the supematant (the stimulating fluid) centrifuged from myeloid cells suspension after low dose radiation in vitro can produce hormesis on the normal or radiation damage cells. The mechanism of free radical was probed. Methods Mouse myeloid cell suspension was irradiated respectively by 0, 2 and 5 Gy, and cultured in vitro. MTT method was used to measure the reproductive activity of cells. Meanwhile, Cytochrome C reduction method was used to determine the concentration of O2-. Lastly, the concentration of O2- was decreased or increased by adding DPI or PMA, and the effect of such changes on "the stimulating fluid" was observed. Results Co-cultured with "the stimulating fluid", the reproductive activity of the myeloid cells after large dose radiation or the normal myeloid cells were enhanced. Decreasing the concentration of O2- may degrade the proliferation of the cells after radiation damage; while increasing it may lead to the opposite result. Conclusions The stimulating fluid can enhance the proliferation of the myeloid cells after radiation damage and also the normal ones. The mechanism of above-mentioned phenomena might be related with the changes of O2- concentration.