国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2010年
2期
105-107
,共3页
六味地黄汤%线粒体%蛋白质组%NF-κB
六味地黃湯%線粒體%蛋白質組%NF-κB
륙미지황탕%선립체%단백질조%NF-κB
Liuwei Dihuang Decoction%Mitochondria%Proteome%Energy metabolism%NF-κB
目的 应用双向电泳技术和质谱技术研究六味地黄汤对甲亢型肾阴虚大鼠肝线粒体蛋向质组的影响.方法 双向电泳分离甲状腺素造成的肾阴虚模型和六味地黄汤治疗大鼠肝线粒体蛋白质,银染后扫描得到蛋白质组图谱,用Image Master2D6.0软件进行差异蛋白质组分析,筛选差异在2倍以上的蛋白质进行质谱鉴定和生物信息学分析.结果 大鼠Major urinary protein precursor、Peroxiredoxin-6、内质网蛋白29前体、大鼠转甲状腺素蛋白D链表达升高,78 kDa葡萄糖调节蛋白前体、谷胱甘肽硫转移酶Mu 1、热休克蛋白60、肽基脯氨酰顺反式异构酶、核转录因子-κB 2、H~+-转运ATP合酶、BNIP1 protein表达均降低.结论 六味地黄汤滋阴补肾作用机理可能与下调线粒体内H~+-转运ATP合酶表达量从而减缓能量代谢、抑制NF-κB过量表达和活化以调节炎性细胞因子紊乱状态和凋亡抑制状态、上调PDX6表达对抗氧自由基损伤以及增强性激素分泌水平相关.
目的 應用雙嚮電泳技術和質譜技術研究六味地黃湯對甲亢型腎陰虛大鼠肝線粒體蛋嚮質組的影響.方法 雙嚮電泳分離甲狀腺素造成的腎陰虛模型和六味地黃湯治療大鼠肝線粒體蛋白質,銀染後掃描得到蛋白質組圖譜,用Image Master2D6.0軟件進行差異蛋白質組分析,篩選差異在2倍以上的蛋白質進行質譜鑒定和生物信息學分析.結果 大鼠Major urinary protein precursor、Peroxiredoxin-6、內質網蛋白29前體、大鼠轉甲狀腺素蛋白D鏈錶達升高,78 kDa葡萄糖調節蛋白前體、穀胱甘肽硫轉移酶Mu 1、熱休剋蛋白60、肽基脯氨酰順反式異構酶、覈轉錄因子-κB 2、H~+-轉運ATP閤酶、BNIP1 protein錶達均降低.結論 六味地黃湯滋陰補腎作用機理可能與下調線粒體內H~+-轉運ATP閤酶錶達量從而減緩能量代謝、抑製NF-κB過量錶達和活化以調節炎性細胞因子紊亂狀態和凋亡抑製狀態、上調PDX6錶達對抗氧自由基損傷以及增彊性激素分泌水平相關.
목적 응용쌍향전영기술화질보기술연구륙미지황탕대갑항형신음허대서간선립체단향질조적영향.방법 쌍향전영분리갑상선소조성적신음허모형화륙미지황탕치료대서간선립체단백질,은염후소묘득도단백질조도보,용Image Master2D6.0연건진행차이단백질조분석,사선차이재2배이상적단백질진행질보감정화생물신식학분석.결과 대서Major urinary protein precursor、Peroxiredoxin-6、내질망단백29전체、대서전갑상선소단백D련표체승고,78 kDa포도당조절단백전체、곡광감태류전이매Mu 1、열휴극단백60、태기포안선순반식이구매、핵전록인자-κB 2、H~+-전운ATP합매、BNIP1 protein표체균강저.결론 륙미지황탕자음보신작용궤리가능여하조선립체내H~+-전운ATP합매표체량종이감완능량대사、억제NF-κB과량표체화활화이조절염성세포인자문란상태화조망억제상태、상조PDX6표체대항양자유기손상이급증강성격소분비수평상관.
Objective Two-dimensional electrophoresis and mass spectrum were used to study the effects of Liuwei Dihuang Decoction on hepatic mitochondria proteome of kidney-yin deficiency rat induced by hyperthyrea. Methods The protein of liver mitochondria from kidney-yin deficiency rats and the kidney-yin deficiency rats treated with Liuwei Dihuang Decoction were isolated by two-dimensional electrophoresis, then stained with silver staining method. The gels were respectively imaged by UMAX ImageScanner and spot-features of proteome difference were analyzed by ImageMaster2D6.0 software. MALDI-TOF MS was used to identify the different protein with abundant changes more than 2 folder between normal rats and kidney-yin deficiency rats. Results In the treated group it was found that major urinary protein precursor, peroxiredoxin-6, endoplasmic reticulum protein 29 precursor, chain D rat transthyretin were increased, while 78 kDa glucos eregurated protein precursor, glutathione S-transferase Mu 1, 60 kDa heat shock protein, peptidyl-prolyl cis-trans isomerase, H~+ -transporting ATP synthase, nuclear factor kappa B 2, BNIP1 protein were decreased. Conclusion The mechanism of nourishing kidney-yin action of Liuwei Dihuang Decoction lies in its relieving energy metabolism, regulating inflammatory factorderangement and apoptosis inhibition, antagonizing free radical damage, enhancing gonadal hormone level by the down regulation of NF-κB, H~+-transporting ATP synthase, and the up-regulation of PDX6.
