国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2012年
1期
18-21
,共4页
王树鹏%郭晓东%李海波%张丽艳%曲道炜
王樹鵬%郭曉東%李海波%張麗豔%麯道煒
왕수붕%곽효동%리해파%장려염%곡도위
变应性鼻炎%白介素-5%肿瘤坏死因子-α%加味小青龙汤
變應性鼻炎%白介素-5%腫瘤壞死因子-α%加味小青龍湯
변응성비염%백개소-5%종류배사인자-α%가미소청룡탕
Rhinitis%Allergic%IL-5%TNF-α%Modified Xiaoqinglong decoction
目的 观察加味小青龙汤及其拆方对变应性鼻炎模型大鼠血浆白介素-5 (IL-5)、肿瘤坏死因子-α(TNF-α)含量的影响,探讨该方治疗变应性鼻炎的作用机制及其组方规律.方法 将加味小青龙汤拆方成益气组、温阳组、化饮组、益气温阳组、益气化饮组、温阳化饮组.将90只大鼠按体重采用随机区组法分成正常对照组、模型组、益气组、温阳组、化饮组、益气温阳组、益气化饮组、温阳化饮组和全方组,每组10只.采用抗原佐剂全身致敏与局部攻击法,制备大鼠变应性鼻炎模型.正常对照组和模型组灌服生理盐水,其他各组分别给予益气汤、温阳汤、化饮汤、益气温阳汤、益气化饮汤、温阳化饮汤和小青龙汤灌胃,1次/d,连续4周.干预前后检测各组大鼠血浆IL-5、TNF-α含量.结果 模型组大鼠血浆IL-5( 16.0±2.7) mg/L、TNF-α (57.5±8.0) mg/L含量明显升高,益气组、温阳组、化饮组、益气温阳组、益气化饮组、温阳化饮组和全方组大鼠血浆IL-5[分别为(12.9±3.1) mg/L、(11.8±2.8) mg/L、(12.0±2.3) mg/L、(12.3±2.3) mg/L、(11.1±2.1) mg/L、(11.2±2.5) mg/L、(8.4± 2.3) mg/L]和TNF-α[分别为(27.7±5.7) mg/L、(29.5±3.7) mg/L、(31.2±4.9) mg/L、(28.1±2.8) mg/L、(33.4± 5.6) mg/L、(26.3±3.9) mg/L、(21.6±4.9) mg/L]含量明显降低,其中全方组减低明显(P＜0.05).结论 加味小青龙汤及其拆方后的各组,均具有不同程度的调节大鼠血浆IL-5、TNF-α含量作用,全方组的温阳、益气、化饮作用最强.
目的 觀察加味小青龍湯及其拆方對變應性鼻炎模型大鼠血漿白介素-5 (IL-5)、腫瘤壞死因子-α(TNF-α)含量的影響,探討該方治療變應性鼻炎的作用機製及其組方規律.方法 將加味小青龍湯拆方成益氣組、溫暘組、化飲組、益氣溫暘組、益氣化飲組、溫暘化飲組.將90隻大鼠按體重採用隨機區組法分成正常對照組、模型組、益氣組、溫暘組、化飲組、益氣溫暘組、益氣化飲組、溫暘化飲組和全方組,每組10隻.採用抗原佐劑全身緻敏與跼部攻擊法,製備大鼠變應性鼻炎模型.正常對照組和模型組灌服生理鹽水,其他各組分彆給予益氣湯、溫暘湯、化飲湯、益氣溫暘湯、益氣化飲湯、溫暘化飲湯和小青龍湯灌胃,1次/d,連續4週.榦預前後檢測各組大鼠血漿IL-5、TNF-α含量.結果 模型組大鼠血漿IL-5( 16.0±2.7) mg/L、TNF-α (57.5±8.0) mg/L含量明顯升高,益氣組、溫暘組、化飲組、益氣溫暘組、益氣化飲組、溫暘化飲組和全方組大鼠血漿IL-5[分彆為(12.9±3.1) mg/L、(11.8±2.8) mg/L、(12.0±2.3) mg/L、(12.3±2.3) mg/L、(11.1±2.1) mg/L、(11.2±2.5) mg/L、(8.4± 2.3) mg/L]和TNF-α[分彆為(27.7±5.7) mg/L、(29.5±3.7) mg/L、(31.2±4.9) mg/L、(28.1±2.8) mg/L、(33.4± 5.6) mg/L、(26.3±3.9) mg/L、(21.6±4.9) mg/L]含量明顯降低,其中全方組減低明顯(P＜0.05).結論 加味小青龍湯及其拆方後的各組,均具有不同程度的調節大鼠血漿IL-5、TNF-α含量作用,全方組的溫暘、益氣、化飲作用最彊.
목적 관찰가미소청룡탕급기탁방대변응성비염모형대서혈장백개소-5 (IL-5)、종류배사인자-α(TNF-α)함량적영향,탐토해방치료변응성비염적작용궤제급기조방규률.방법 장가미소청룡탕탁방성익기조、온양조、화음조、익기온양조、익기화음조、온양화음조.장90지대서안체중채용수궤구조법분성정상대조조、모형조、익기조、온양조、화음조、익기온양조、익기화음조、온양화음조화전방조,매조10지.채용항원좌제전신치민여국부공격법,제비대서변응성비염모형.정상대조조화모형조관복생리염수,기타각조분별급여익기탕、온양탕、화음탕、익기온양탕、익기화음탕、온양화음탕화소청룡탕관위,1차/d,련속4주.간예전후검측각조대서혈장IL-5、TNF-α함량.결과 모형조대서혈장IL-5( 16.0±2.7) mg/L、TNF-α (57.5±8.0) mg/L함량명현승고,익기조、온양조、화음조、익기온양조、익기화음조、온양화음조화전방조대서혈장IL-5[분별위(12.9±3.1) mg/L、(11.8±2.8) mg/L、(12.0±2.3) mg/L、(12.3±2.3) mg/L、(11.1±2.1) mg/L、(11.2±2.5) mg/L、(8.4± 2.3) mg/L]화TNF-α[분별위(27.7±5.7) mg/L、(29.5±3.7) mg/L、(31.2±4.9) mg/L、(28.1±2.8) mg/L、(33.4± 5.6) mg/L、(26.3±3.9) mg/L、(21.6±4.9) mg/L]함량명현강저,기중전방조감저명현(P＜0.05).결론 가미소청룡탕급기탁방후적각조,균구유불동정도적조절대서혈장IL-5、TNF-α함량작용,전방조적온양、익기、화음작용최강.
Objective To observe the modified Xiaoqinglong decoction and its ingredient-reduced prescription on plasma levels of IL-5 and TNF-ct in rats,and to explore its mechanism of treating AR.To make clear the law of combination in modified Xiaoqinglong decoction.Methods The modified Xiaoqinglong decoction was divided into six groups,namely,reinforcing qi group,warming yang group,expelling retained morbid fluid group,reinforcing qi and warming yang group,reinforcing qi and expelling retained morbid fluid group,warming yang to expel retained morbid fluid group.90 rats were randomly divided into nine groups,namely,normal control group,model group,reinforcing qi group,warming yang group,expelling retained morbid fluid group,reinforcing qi and warming yang group,reinforcing qi and expelling retained morbid fluid group,warming yang to expel retained morbid fluid group,and the whole decoction group,with 10 rats in each group.Rat model of allergic rhinitis was made by the use of adjuvant systemic antigen sensitization and local attack,the normal control group and model group were fed with normal saline,the other groups were treated with appropriate drugs,once a day oral administration,continuous for 4 weeks.Detected plasma IL-5 and TNF-α levels of each group in rats.Results The contents of plasma IL-5 (16.0±2.7)mg/L and TNF-α (57.5±8.0)mg/L in the model group was significantly increased,while it was significantly reduced in the reinforcing qi group,warming yang group,expelling retained morbid fluid group,refinorcing qi and warming yang group,reinforcing qi and expelling retained morbid fluid group,warming yang to expel retained morbid fluid group with plasma IL-5[each group was (12.9± 3.1) mg/L、(11.8 ±2.8) mg/L、(12.0±2.3) mg/L、(12.3±2.3) mg/L、(11.1±2.1)mg/L、(11.2±2.5)mg/L、(8.42.3)mg/L respectively]and TNF-α[each group was (27.7±5.7)mg/L、(29.5 ± 3.7) mg/L、(31.2 ± 4.9) mg/L、(28.1 ± 2.8) mg/L、(33.4 ± 5.6) mg/L、(26.3 ± 3.9) mg/L、(21.6 ±4.9) mg/L respectively],the whole decoction group effect was significant compared with the other treated groups (P＜0.05).Conclusion The modified Xiaoqinglong decoction and its ingredient-reduced prescription can regulate plasma levels of IL-5 and TNF-α.The modified Xiaoqinglong decoction had best results in reinforcing qi,warming yang and expelling retained morbid fluid.
