中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2011年
6期
518-521
,共4页
动脉粥样硬化%炎症%脂蛋白类,LDL%环氧化酶2%缬沙坦
動脈粥樣硬化%炎癥%脂蛋白類,LDL%環氧化酶2%纈沙坦
동맥죽양경화%염증%지단백류,LDL%배양화매2%힐사탄
Atherosclerosis%Inflammation%Lipoprotein,LDL%Cyclooxygenase 2%Valsartan
目的 检测经氧化低密度脂蛋白(ox-LDL)干预后内皮细胞环氧合酶-2(COX-2)mRNA的表达,观察缬沙坦对COX-2 mRNA表达的影响及意义.方法 待体外培养的人脐静脉内皮细胞生长至融合状态时,进行分组处理,每组n=5.正常对照组,不加任何处理;ox-LDL组,加入ox-LDL培养24 h,终浓度为100 mg/L;ox-LDL组+低浓度缬沙坦组,加入ox-LDL(浓度100 mg/L)及缬沙坦(浓度10 μmol/L)共同培养24 h;ox-LDL +高浓度缬沙坦组,加入ox-LDL(浓度100 mg/L)及缬沙坦(浓度30 μmol/L)共同培养24 h.采用逆转录聚合酶链反应(RT-PCR)分别测定COX-1 mRNA及COX-2 mRNA的表达.结果 ox-LDL对COX-1 mRNA的表达无诱导作用,各组间差异均无统计学意义(P均＞0.05).ox-LDL可诱导COX-2 mRNA的表达,ox-LDL组与对照组比较差异有统计学意义(1.478±0.104比0.366±0.104,P<0.05).不同浓度的缬沙坦可抑制ox-LDL诱导的COX-2 mRNA的表达,并呈浓度依赖性[ox-LDL组+低浓度缬沙坦组(1.074±0.112)及ox-LDL组+高浓度缬沙坦组(0.664±0.104)与ox-LDL组(1.478±0.104)比较差异均有统计学意义,P均<0.05].缬沙坦对COX-1 mRNA表达无明显抑制作用.结论 COX-2在动脉粥样斑块形成中可能起着重要的作用,缬沙坦抑制了人脐静脉内皮细胞COX-2 mRNA的表达,表明其可能具有抗炎及抗动脉粥样硬化的作用.
目的 檢測經氧化低密度脂蛋白(ox-LDL)榦預後內皮細胞環氧閤酶-2(COX-2)mRNA的錶達,觀察纈沙坦對COX-2 mRNA錶達的影響及意義.方法 待體外培養的人臍靜脈內皮細胞生長至融閤狀態時,進行分組處理,每組n=5.正常對照組,不加任何處理;ox-LDL組,加入ox-LDL培養24 h,終濃度為100 mg/L;ox-LDL組+低濃度纈沙坦組,加入ox-LDL(濃度100 mg/L)及纈沙坦(濃度10 μmol/L)共同培養24 h;ox-LDL +高濃度纈沙坦組,加入ox-LDL(濃度100 mg/L)及纈沙坦(濃度30 μmol/L)共同培養24 h.採用逆轉錄聚閤酶鏈反應(RT-PCR)分彆測定COX-1 mRNA及COX-2 mRNA的錶達.結果 ox-LDL對COX-1 mRNA的錶達無誘導作用,各組間差異均無統計學意義(P均＞0.05).ox-LDL可誘導COX-2 mRNA的錶達,ox-LDL組與對照組比較差異有統計學意義(1.478±0.104比0.366±0.104,P<0.05).不同濃度的纈沙坦可抑製ox-LDL誘導的COX-2 mRNA的錶達,併呈濃度依賴性[ox-LDL組+低濃度纈沙坦組(1.074±0.112)及ox-LDL組+高濃度纈沙坦組(0.664±0.104)與ox-LDL組(1.478±0.104)比較差異均有統計學意義,P均<0.05].纈沙坦對COX-1 mRNA錶達無明顯抑製作用.結論 COX-2在動脈粥樣斑塊形成中可能起著重要的作用,纈沙坦抑製瞭人臍靜脈內皮細胞COX-2 mRNA的錶達,錶明其可能具有抗炎及抗動脈粥樣硬化的作用.
목적 검측경양화저밀도지단백(ox-LDL)간예후내피세포배양합매-2(COX-2)mRNA적표체,관찰힐사탄대COX-2 mRNA표체적영향급의의.방법 대체외배양적인제정맥내피세포생장지융합상태시,진행분조처리,매조n=5.정상대조조,불가임하처리;ox-LDL조,가입ox-LDL배양24 h,종농도위100 mg/L;ox-LDL조+저농도힐사탄조,가입ox-LDL(농도100 mg/L)급힐사탄(농도10 μmol/L)공동배양24 h;ox-LDL +고농도힐사탄조,가입ox-LDL(농도100 mg/L)급힐사탄(농도30 μmol/L)공동배양24 h.채용역전록취합매련반응(RT-PCR)분별측정COX-1 mRNA급COX-2 mRNA적표체.결과 ox-LDL대COX-1 mRNA적표체무유도작용,각조간차이균무통계학의의(P균＞0.05).ox-LDL가유도COX-2 mRNA적표체,ox-LDL조여대조조비교차이유통계학의의(1.478±0.104비0.366±0.104,P<0.05).불동농도적힐사탄가억제ox-LDL유도적COX-2 mRNA적표체,병정농도의뢰성[ox-LDL조+저농도힐사탄조(1.074±0.112)급ox-LDL조+고농도힐사탄조(0.664±0.104)여ox-LDL조(1.478±0.104)비교차이균유통계학의의,P균<0.05].힐사탄대COX-1 mRNA표체무명현억제작용.결론 COX-2재동맥죽양반괴형성중가능기착중요적작용,힐사탄억제료인제정맥내피세포COX-2 mRNA적표체,표명기가능구유항염급항동맥죽양경화적작용.
Objective To investigate the effects of valsartan on cyclooxygenase-2 (COX-2) in cultured human umbilical vein endothelial cells (HUVECs) stimulated by ox-LDL.Methods HUVECs were cultured in endothelial basal medium and divided into four groups (n=5 each):group Ⅰ, control group without any treatment; groupⅡ: HUVECs stimulated with ox-LDL(100 mg/L)in endothelial basal medium for 24 hours; groupⅢ: HUVECs treated with ox-LDL (100 mg/L)and valsartan(10 μmol/L) in endothelial basal medium for 24 hours; groupⅣ:HUVECs treated with ox-LDL(100 mg/L)and valsartan(30 μmol/L) in endothelial basal medium for 24 hours. Expression of COX-1 and COX-2 mRNA were determined by reverse transcription-polymeras chain reaction(RT-PCR).Results Expression of and COX-2 mRNA was significantly higher in ox-LDL-treated HUVECs than in control group (1.478±0.104 vs. 0.366±0.104,P<0.05) ,while expression of COX-1 mRNA was similar between the 2 groups (P＞0.05). Valsartan dose-dependently decreased the COX-2 mRNA expression (group Ⅲ vs. groupⅡ:1.074±0.112 vs. 1.478±0.104,P<0.05; group Ⅳ vs. groupⅡ: 0.664±0.104 vs.1.478±0.104, P<0.05). Expression of COX-1 mRNA in ox-LDL-treated HUVECs was not affected by valsartan.Conclusions COX-2 mRNA expression in ox-LDL-treated HUVECs could be reduced by valsartan suggesting valsartan might attenuate atherosclerosis by reducing ox-LDL-induced inflammatory responses.
