中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2008年
4期
248-251
,共4页
电针%急性力竭运动%一氧化氮合酶%肌间神经丛%大鼠
電針%急性力竭運動%一氧化氮閤酶%肌間神經叢%大鼠
전침%급성력갈운동%일양화담합매%기간신경총%대서
Electroacupuncture%Acute exhaustive exercise%Nitric oxide synthase%Myenteric plexus%Rat
目的 研究电针足三里穴对急性力竭运动大鼠胃肠动力和肌间神经丛一氧化氮合酶(NOS)阳性神经元的影响.方法 将54只雄性Sprague-Dawley大鼠随机分成急性力竭运动组(运动组)、急性力竭运动+电针组(电针组)和安静对照组(对照组).运动组和电针组建立大鼠急性力竭运动模型,电针组采用电针刺激大鼠双侧足三里穴.测定胃排空和小肠推进速率,同时用酶组织化学方法 和计算机图像分析技术对大鼠回肠肌间神经丛内NOS阳性神经元的数目和NOS的表达进行测定.结果 与对照组相比,运动组大鼠胃内色素残留量明显增多(胃排空减慢),小肠推进速率明显降低,回肠肌间神经丛内NOS阳性神经元的数目和NOS的表达明显增高(P＜0.01);与运动组相比,电针组大鼠胃内色素残留量显著降低(胃排空加快),小肠推进速率明显加快,回肠肌间神经丛内NOS阳性神经元的数目和NOS的表达明显下降(P＜0.01).结论 急性力竭运动大鼠肌间神经丛NOS阳性神经元的数目和NOS的表达增高可能是导致胃肠运动减慢的重要原因之一;电针作用能使急性力竭运动大鼠肌间神经丛NOS阳性神经元数目减少,下调NOS的表达,并促进其胃肠运动.
目的 研究電針足三裏穴對急性力竭運動大鼠胃腸動力和肌間神經叢一氧化氮閤酶(NOS)暘性神經元的影響.方法 將54隻雄性Sprague-Dawley大鼠隨機分成急性力竭運動組(運動組)、急性力竭運動+電針組(電針組)和安靜對照組(對照組).運動組和電針組建立大鼠急性力竭運動模型,電針組採用電針刺激大鼠雙側足三裏穴.測定胃排空和小腸推進速率,同時用酶組織化學方法 和計算機圖像分析技術對大鼠迴腸肌間神經叢內NOS暘性神經元的數目和NOS的錶達進行測定.結果 與對照組相比,運動組大鼠胃內色素殘留量明顯增多(胃排空減慢),小腸推進速率明顯降低,迴腸肌間神經叢內NOS暘性神經元的數目和NOS的錶達明顯增高(P＜0.01);與運動組相比,電針組大鼠胃內色素殘留量顯著降低(胃排空加快),小腸推進速率明顯加快,迴腸肌間神經叢內NOS暘性神經元的數目和NOS的錶達明顯下降(P＜0.01).結論 急性力竭運動大鼠肌間神經叢NOS暘性神經元的數目和NOS的錶達增高可能是導緻胃腸運動減慢的重要原因之一;電針作用能使急性力竭運動大鼠肌間神經叢NOS暘性神經元數目減少,下調NOS的錶達,併促進其胃腸運動.
목적 연구전침족삼리혈대급성력갈운동대서위장동력화기간신경총일양화담합매(NOS)양성신경원적영향.방법 장54지웅성Sprague-Dawley대서수궤분성급성력갈운동조(운동조)、급성력갈운동+전침조(전침조)화안정대조조(대조조).운동조화전침조건립대서급성력갈운동모형,전침조채용전침자격대서쌍측족삼리혈.측정위배공화소장추진속솔,동시용매조직화학방법 화계산궤도상분석기술대대서회장기간신경총내NOS양성신경원적수목화NOS적표체진행측정.결과 여대조조상비,운동조대서위내색소잔류량명현증다(위배공감만),소장추진속솔명현강저,회장기간신경총내NOS양성신경원적수목화NOS적표체명현증고(P＜0.01);여운동조상비,전침조대서위내색소잔류량현저강저(위배공가쾌),소장추진속솔명현가쾌,회장기간신경총내NOS양성신경원적수목화NOS적표체명현하강(P＜0.01).결론 급성력갈운동대서기간신경총NOS양성신경원적수목화NOS적표체증고가능시도치위장운동감만적중요원인지일;전침작용능사급성력갈운동대서기간신경총NOS양성신경원수목감소,하조NOS적표체,병촉진기위장운동.
Objective To study the effects of electroacupuncture(EA)on gastrointestinal motility and nitric oxide synthase(NOS)positive nerves in myenteric plexus in acute exhaustive exercise rats. Methods A total of 54 male Sprague-Dawley rats were randomly divided into an acute exhaustive exercise(AEE)group,an AEE plus EA group and a control(C)group.The gastric empty rate and intestinal motility rate were measured,and histological changes of NOS-positive nerves in ileum myenteric plexus were observed with enzymatic histochemical method and image analysis technique. Results In the rats of AEE group,the gastric empty rate and intestinal motility rate were delayed but the numbers of NOS-positive neurons and expression levels of NOS in the ileum myenteric plexus significantly increased comparing with those of the C group(P＜0.01).Compared with AEE group,the gastric empty rate and intestinal motility rate were significantly increased but the number of NOS-positive neurons and expression level of NOS in the ileum myenteric plexus significantly decreased in AEE plus EA group(P＜0.01). Conclusion The findings of this study suggest that increase of NOS-positive neurons and expression levels of NOS in the myenteric plexus is one of the mechanisms of slowing down of gastrointestinal movement after acute exhaustive exercise.Electroacupuncture at Zusanli acupoint may decrease the number of NOS-positive neurons and expression levels of NOS in the myenteric plexus,and improve gastrointestinal movement in acute exhaustive exercise rats.
