CAJ | 학술논문

通过细胞提取液,在体外条件下研究了辣根过氧化物酶对青蒿素生物合成的影响.细胞提取液以磷酸与Tris两种缓冲液制备.结果表明,辣根过氧化物酶在以磷酸缓冲液制备的细胞提取液中,很大地促进了青蒿素的生物合成,青蒿素的含量提高约1倍左右,而外加青蒿酸对青蒿素的生物合成并无显著的影响.以Tris缓冲液制备细胞提取液,外加过氧化物酶并不能促进青蒿素的生物合成. 图4 参12
통과세포제취액,재체외조건하연구료랄근과양화물매대청호소생물합성적영향.세포제취액이린산여Tris량충완충액제비.결과표명,랄근과양화물매재이린산완충액제비적세포제취액중,흔대지촉진료청호소적생물합성,청호소적함량제고약1배좌우,이외가청호산대청호소적생물합성병무현저적영향.이Tris완충액제비세포제취액,외가과양화물매병불능촉진청호소적생물합성. 도4 삼12
In this paper, the effect of horseradish peroxidase on the biosynthesis of artemisinin was studied. Two buffer systems were used to prepare cell free extracts. The results showed that the level of artemisinin was elevated to about 1-fold when the peroxidase was included in cell free extracts by phosphate buffer system, and the added exogenous artemisinic acid could not greatly affect the production of artemisinin. This study indicated that phosphate buffer system was better for peroxidase activities than Tris buffer system. In the cell free extracts by Tris buffer system, addition of horseradish peroxidase did not promote the biosynthesis of artemisinin. Fig 4, Ref 12