CAJ | 학술논문

以麻疯树上胚轴为实验材料在MS添加IBA和BA的培养基上进行离体培养实验.结果表明,在IBA O.1 mg/L与BA 0.2～0.7 mg/L组合的条件下,不定芽从上胚轴外植体的表面直接被诱导分化,其中以在MS+IBA 0.1 mg/L+BA 0.5 mg/L上的诱导率最高.从愈伤组织来源的植株再生需要IBA 0.5 mg/L与BA 0.1 mg/L、IBA 0.5 mg/L与BA0.2mg/L以及IBA1.0mg/L与BA0.5mg/L的激素组合,其分化的最佳培养基是MS+IBA1.0 mg/L+BA0.5 mg/L.生长健壮的不定芽和再生植株能在无激素的MS基本培养基上生根.发育良好的再生苗可成功地转移到温室栽培而没有可见的变异.
이마풍수상배축위실험재료재MS첨가IBA화BA적배양기상진행리체배양실험.결과표명,재IBA O.1 mg/L여BA 0.2～0.7 mg/L조합적조건하,불정아종상배축외식체적표면직접피유도분화,기중이재MS+IBA 0.1 mg/L+BA 0.5 mg/L상적유도솔최고.종유상조직래원적식주재생수요IBA 0.5 mg/L여BA 0.1 mg/L、IBA 0.5 mg/L여BA0.2mg/L이급IBA1.0mg/L여BA0.5mg/L적격소조합,기분화적최가배양기시MS+IBA1.0 mg/L+BA0.5 mg/L.생장건장적불정아화재생식주능재무격소적MS기본배양기상생근.발육량호적재생묘가성공지전이도온실재배이몰유가견적변이.
In vitro epicotyl explants from Jatropha curcas were cultured on MS medium with indole-3-butyric acid (IBA) and 6-benzyladenine (BA). Adventitious buds were directly induced from the surface of epicotyl explants under the condition with the combinations of IBA 0.1 mg/L and BA 0.2-0.7 mg/L. Of which IBA 0.1 mg/L and BA 0.5 mg/L induced the highest regeneration frequency. Shoot regeneration from callus required the combinations of IBA 0.5 mg/L with BA 0.1 mg/L, IBA 0.5 mg/L with BA 0.2 mg/L and IBA 1.0 mg/L with BA 0.5 mg/L, and the most suitable combination was IBA 1.0 mg/L and BA 0.5 mg/L. The health adventitious buds and regenerated shoots could be rooted on growth regulator-free MS medium. Regenerated plants with well developed shoots and roots were successfully transferred to greenhouse, without visible detectable variation.