中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2009年
7期
538-542,512
,共6页
杨开初%廖二元%罗湘杭%周后德
楊開初%廖二元%囉湘杭%週後德
양개초%료이원%라상항%주후덕
尼尔雌醇%左炔诺孕酮%雌激素受体亚型%原位RT-PCR%激光共聚焦显微镜
尼爾雌醇%左炔諾孕酮%雌激素受體亞型%原位RT-PCR%激光共聚焦顯微鏡
니이자순%좌결낙잉동%자격소수체아형%원위RT-PCR%격광공취초현미경
Nylestriol%Levonorgestrel%Estrogen receptor subtypes%In situ RT-PCR%Laser scanning confocal microscopy
目的 观察NYL(尼尔雌醇)联合LNG(左炔诺孕酮)干预培养人成骨肉瘤MG-63细胞株在雌激素受体(ER)亚型mRNA表达过程中是否存在正性协同作用.方法 用直接原位RT-PCR及激光共聚焦显微镜观察不同药物干预组的MG-63细胞株ERα、ERβ mRNA表达.结果 NYL及LNG两种药物联合干预培养组的ERα或ERβ的荧光强度均明显超过两种药物单独干预培养组.结论 NYL和LNG在诱导MG-63细胞株ER亚型表达上调过程中具有正性协同作用,LNG可具有独立预防骨质疏松作用.
目的 觀察NYL(尼爾雌醇)聯閤LNG(左炔諾孕酮)榦預培養人成骨肉瘤MG-63細胞株在雌激素受體(ER)亞型mRNA錶達過程中是否存在正性協同作用.方法 用直接原位RT-PCR及激光共聚焦顯微鏡觀察不同藥物榦預組的MG-63細胞株ERα、ERβ mRNA錶達.結果 NYL及LNG兩種藥物聯閤榦預培養組的ERα或ERβ的熒光彊度均明顯超過兩種藥物單獨榦預培養組.結論 NYL和LNG在誘導MG-63細胞株ER亞型錶達上調過程中具有正性協同作用,LNG可具有獨立預防骨質疏鬆作用.
목적 관찰NYL(니이자순)연합LNG(좌결낙잉동)간예배양인성골육류MG-63세포주재자격소수체(ER)아형mRNA표체과정중시부존재정성협동작용.방법 용직접원위RT-PCR급격광공취초현미경관찰불동약물간예조적MG-63세포주ERα、ERβ mRNA표체.결과 NYL급LNG량충약물연합간예배양조적ERα혹ERβ적형광강도균명현초과량충약물단독간예배양조.결론 NYL화LNG재유도MG-63세포주ER아형표체상조과정중구유정성협동작용,LNG가구유독립예방골질소송작용.
Objective To determine whether positive synergistic effect is present on inducing the up-regulation of mRNA expression of ER subtypes by combined intervention with NYL and LNG.Methods Direct In situ RT-PCR combined with LSCM was applied to observe the mRNA expression of ERα and ERβ in MG-63 cell lines which were cultured with NYL and/or LNG intervention.Results The fluorescence intensity of both ER subtypes on the slides which were processed with combined intervention MG-63 cells are much more intense than that of ER subtypes on the slides which were processed with alone intervention.Conclusion Combined intervention with NYL and LNG has positive synergistic effects on the up-regulation of mRNA expression of ER subtypes in MG-63 cell lines. LNG maybe have the effects of preventing postmenopausal women suffering bone loss.
