西南农业学报
西南農業學報
서남농업학보
SOUTHWEST CHINA JOURNAL OF AGRICULTURAL SCIENCES
2009年
5期
1435-1438
,共4页
高效液相色谱%SNAP快速检测%黄曲霉毒素M_1%牛奶
高效液相色譜%SNAP快速檢測%黃麯黴毒素M_1%牛奶
고효액상색보%SNAP쾌속검측%황곡매독소M_1%우내
High performance liquid chromatography(HPLC)%SNAP rapid detecting%Aflatoxins M_1%Milk
用SNAP快速检测法与高效液相色谱法对牛奶样品中黄曲霉毒素M_1的检测,首先运用SNAP快速法对牛奶样品中的黄曲霉毒素M_1进行初筛,其中的阳性样品通过免疫亲和层析柱净化,并用带荧光检测器的高效液相色谱仪进行定性定量检测,从而比较SNAP快速法与高效液相色谱法之间的差异性及相关性.通过比较,SNAP快速法虽然简单、快捷,但检测结果有假阳性现象;液相色谱法前处理、分析过程较复杂,但灵敏度高,检测限低,回收率及线性关系好,结果准确可靠.
用SNAP快速檢測法與高效液相色譜法對牛奶樣品中黃麯黴毒素M_1的檢測,首先運用SNAP快速法對牛奶樣品中的黃麯黴毒素M_1進行初篩,其中的暘性樣品通過免疫親和層析柱淨化,併用帶熒光檢測器的高效液相色譜儀進行定性定量檢測,從而比較SNAP快速法與高效液相色譜法之間的差異性及相關性.通過比較,SNAP快速法雖然簡單、快捷,但檢測結果有假暘性現象;液相色譜法前處理、分析過程較複雜,但靈敏度高,檢測限低,迴收率及線性關繫好,結果準確可靠.
용SNAP쾌속검측법여고효액상색보법대우내양품중황곡매독소M_1적검측,수선운용SNAP쾌속법대우내양품중적황곡매독소M_1진행초사,기중적양성양품통과면역친화층석주정화,병용대형광검측기적고효액상색보의진행정성정량검측,종이비교SNAP쾌속법여고효액상색보법지간적차이성급상관성.통과비교,SNAP쾌속법수연간단、쾌첩,단검측결과유가양성현상;액상색보법전처리、분석과정교복잡,단령민도고,검측한저,회수솔급선성관계호,결과준학가고.
Aflatoxins M1 in milk was detected by SNAP rapid detecting and HPLC. SNAP rapid detecting was used to do preliminary screening of aflatoxins M_1, and then positive samples were purified by immuno-affinity chromatography and qualitative and quantitative measured by HPLC with fluorescence detector. Compared SNAP rapid detecting with HPLC method, the advantage of SNAP was more simpler and quicker, but the false positive rate was higher; the advantage of HPLC was more sensitive and reliable, and obtained better recovery rate and linear relationship, but the analysis process was more complex.