北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2010年
2期
120-122
,共3页
毛细管气相色谱法%藿香正气软胶囊%百秋李醇%含量测定
毛細管氣相色譜法%藿香正氣軟膠囊%百鞦李醇%含量測定
모세관기상색보법%곽향정기연효낭%백추리순%함량측정
capillary gas chromatography%Huoxiangzhengqi Soft-Capsules%patchouli alcohol%quantitative determination
目的 建立毛细管气相色谱测定藿香正气软胶囊中百秋李醇含量的方法 .方法 样品经超声、离心后用毛细管气相色谱法测定.色谱条件:HP-5色谱柱(30 m×0.32 mm×0.25 μm);初始温度170 ℃,保持3 min,以1 ℃/min的速度升至182 ℃,保持1 min,进样口温度280 ℃,检测器280 ℃,氮气流速0.6 mL/min.以正十八烷为内标,FID检测.结果 百秋李醇在0.041 1～0.411 2 g/L浓度范围内,峰面积与内标峰面积之比值与浓度呈良好的线性关系(Y=2.94X+0.0128,r=0.999 2);平均回收率为98.96%,RSD=1.88%(n=6).结论 本方法 准确、简便、灵敏、快速,可用于测定藿香正气软胶囊中百秋李醇的含量.
目的 建立毛細管氣相色譜測定藿香正氣軟膠囊中百鞦李醇含量的方法 .方法 樣品經超聲、離心後用毛細管氣相色譜法測定.色譜條件:HP-5色譜柱(30 m×0.32 mm×0.25 μm);初始溫度170 ℃,保持3 min,以1 ℃/min的速度升至182 ℃,保持1 min,進樣口溫度280 ℃,檢測器280 ℃,氮氣流速0.6 mL/min.以正十八烷為內標,FID檢測.結果 百鞦李醇在0.041 1～0.411 2 g/L濃度範圍內,峰麵積與內標峰麵積之比值與濃度呈良好的線性關繫(Y=2.94X+0.0128,r=0.999 2);平均迴收率為98.96%,RSD=1.88%(n=6).結論 本方法 準確、簡便、靈敏、快速,可用于測定藿香正氣軟膠囊中百鞦李醇的含量.
목적 건립모세관기상색보측정곽향정기연효낭중백추리순함량적방법 .방법 양품경초성、리심후용모세관기상색보법측정.색보조건:HP-5색보주(30 m×0.32 mm×0.25 μm);초시온도170 ℃,보지3 min,이1 ℃/min적속도승지182 ℃,보지1 min,진양구온도280 ℃,검측기280 ℃,담기류속0.6 mL/min.이정십팔완위내표,FID검측.결과 백추리순재0.041 1～0.411 2 g/L농도범위내,봉면적여내표봉면적지비치여농도정량호적선성관계(Y=2.94X+0.0128,r=0.999 2);평균회수솔위98.96%,RSD=1.88%(n=6).결론 본방법 준학、간편、령민、쾌속,가용우측정곽향정기연효낭중백추리순적함량.
Objective To establish a method of quantitative determination of patchouli alcohol in Huoxiangzhengqi Soft-Capsules by capillary gas chromatography.Methods The samples of patchouli alcohol were determined by capillary gas chromatography after ultrasound treatment and centrifugation. The chromatographic procedure was performed on the column of HP-5 (30 m×0.32 mm×0.25 μm). The initial temperature was maintained at 170 ℃ for 3 minutes, and then the temperature was raised to 182 ℃ at the speed of 1 ℃/min and maintained for 1 minute. The inlet temperature of samples was at 280 ℃, the detector temperature was at 280 ℃, the flow rate of Nitrogen was 0.6 mL/min, n-octadecane was taken as the internal standard, and FID was used for detection. Results Patchouli alcohol showed a good linear relationship at the range from 0.041 1 mg/mL to 0.411 2 mg/mL in the ratio between peak area and internal standard peak area or concentration (Y=2.94X+0.0128, r=0.999 2). The average recovery was 98.96% (RSD=1.88%, n=6).Conclusion The method is accurate, easy and sensitive, which can be used for the quantitative determination of patchouli alcohol in Huoxiangzhengqi Soft-Capsules.
