北京化工大学学报（自然科学版）
北京化工大學學報（自然科學版）
북경화공대학학보（자연과학판）
JOURNAL OF BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY(NATURAL SCIENCE EDITION)
2010年
3期
97-101
,共5页
苏建树%刘白宁%田平芳%林强%赵有玺%葛喜珍
囌建樹%劉白寧%田平芳%林彊%趙有璽%葛喜珍
소건수%류백저%전평방%림강%조유새%갈희진
川乌%附子%微生物发酵%乌头碱%新乌头碱%次乌头碱%液相色谱
川烏%附子%微生物髮酵%烏頭堿%新烏頭堿%次烏頭堿%液相色譜
천오%부자%미생물발효%오두감%신오두감%차오두감%액상색보
radix aconiti%aconite%microbial fermentation%mesaconitine%aconitine%hypaconitine%HPLC
探讨了微生物发酵对川乌、附子中新乌头碱、乌头碱、次乌头碱含量的影响.将菌种以孢子悬液的形式接入灭菌生川乌、生附子、制川乌、制附子药材中,30℃固态发酵培养7d提取牛物碱,对照组为未发酵药材.采用高效液相色谱法测定其含量.研究发现发酵药材中新乌头碱、乌头碱、次乌头碱质量分数明显低于原药材:生川乌中新乌头碱、乌头碱、次乌头碱的质量分数分别为0.1390%、0.0195%、0.0320%,而发酵生川乌中分别为0.0231%、0.0039%、0.0117%;生附子中新乌头碱、乌头碱、次乌头碱的质量分数分别为0.2750%、0.0670%、0.0550%,而发酵生附子中分别为0.0633%、0.0552%、0.0291%;生川乌+附子中新乌头碱、乌头碱、次乌头碱的质量分数分别为0.1395%、0.0075%、0.0078%,而发酵生川乌+附子中分别为0.0202%、0.0022%、0.0026%.该结果表明微生物发酵有助于降低川乌、附子中有毒生物碱的含量.
探討瞭微生物髮酵對川烏、附子中新烏頭堿、烏頭堿、次烏頭堿含量的影響.將菌種以孢子懸液的形式接入滅菌生川烏、生附子、製川烏、製附子藥材中,30℃固態髮酵培養7d提取牛物堿,對照組為未髮酵藥材.採用高效液相色譜法測定其含量.研究髮現髮酵藥材中新烏頭堿、烏頭堿、次烏頭堿質量分數明顯低于原藥材:生川烏中新烏頭堿、烏頭堿、次烏頭堿的質量分數分彆為0.1390%、0.0195%、0.0320%,而髮酵生川烏中分彆為0.0231%、0.0039%、0.0117%;生附子中新烏頭堿、烏頭堿、次烏頭堿的質量分數分彆為0.2750%、0.0670%、0.0550%,而髮酵生附子中分彆為0.0633%、0.0552%、0.0291%;生川烏+附子中新烏頭堿、烏頭堿、次烏頭堿的質量分數分彆為0.1395%、0.0075%、0.0078%,而髮酵生川烏+附子中分彆為0.0202%、0.0022%、0.0026%.該結果錶明微生物髮酵有助于降低川烏、附子中有毒生物堿的含量.
탐토료미생물발효대천오、부자중신오두감、오두감、차오두감함량적영향.장균충이포자현액적형식접입멸균생천오、생부자、제천오、제부자약재중,30℃고태발효배양7d제취우물감,대조조위미발효약재.채용고효액상색보법측정기함량.연구발현발효약재중신오두감、오두감、차오두감질량분수명현저우원약재:생천오중신오두감、오두감、차오두감적질량분수분별위0.1390%、0.0195%、0.0320%,이발효생천오중분별위0.0231%、0.0039%、0.0117%;생부자중신오두감、오두감、차오두감적질량분수분별위0.2750%、0.0670%、0.0550%,이발효생부자중분별위0.0633%、0.0552%、0.0291%;생천오+부자중신오두감、오두감、차오두감적질량분수분별위0.1395%、0.0075%、0.0078%,이발효생천오+부자중분별위0.0202%、0.0022%、0.0026%.해결과표명미생물발효유조우강저천오、부자중유독생물감적함량.
To study the effects of microbial fermentation on the extraction of mesaconitine,aconitine,and hypaconitine from the medicinal materials radix aconiti,aconite and their fermentation products,a strain was inoculated in sterilized radix aconiti and aconite and fermented at 30℃ for 7 days.Subsequently,the alkaloids were extracted and monitored by HPLC,with the materials without fermentation being used as the control.The results showed that the contents of mesaeonitine,aconitine and hypaconitine in the fermented materials were significantly lower than the corresponding values for the control.The contents of mesaconitine,aconitine and hypaconitine in crude radix aconiti were 0.1390%,0.0195% and 0.0320%,respectively,whereas the contents in fermented radix aconiti were 0.023%,0.0039%,and 0.0117%,respectively.Similarly,the contents of mesaconitine,aconitine and hypaconitine in crude aconite were 0.2750%,0.0670% and 0.0550%,respectively,whereas the corresponding values in fermented crude aconite were 0.0633%,0.0552% and 0.0291%,respectively.These results highlight the role of microbial fermentation in reducing the alkaloid content of the material via a bioconversion mechanism.
