CAJ | 학술논문

对异枝麒麟菜硫酸酯多糖(ESSP)的超声辅助提取工艺进行研究,通过单因素试验和正交试验对该提取工艺进行优化.确定最佳工艺条件为超声时间20min、超声功率150W、料液(水)比1:90(g/mL),多糖的提取率达48.14%,所得的多糖含最96.94%,硫酸酯基含量19.66%.并与热水提取法进行比较,采刚优化工艺提取的多糖提取率、多糖含量和硫酸酯基含量比热水提取法分别提高12.08%、8.07%和1.41%.两种方法所得ESSP均不含蛋白质和核酸,红外光谱显示其均含有糖的特征峰和硫酸酯基特征峰.
대이지기린채류산지다당(ESSP)적초성보조제취공예진행연구,통과단인소시험화정교시험대해제취공예진행우화.학정최가공예조건위초성시간20min、초성공솔150W、료액(수)비1:90(g/mL),다당적제취솔체48.14%,소득적다당함최96.94%,류산지기함량19.66%.병여열수제취법진행비교,채강우화공예제취적다당제취솔、다당함량화류산지기함량비열수제취법분별제고12.08%、8.07%화1.41%.량충방법소득ESSP균불함단백질화핵산,홍외광보현시기균함유당적특정봉화류산지기특정봉.
One-factor-at-a-time and orthogonal array methods were used to investigate the optimal ultrasonic-assisted extraction process of sulfated polysaccharides from Eucheuma striatum (ESSP) in this work.Results indicated that the optimal values of technological parameters,including ultrasonic treatment time and power and material/liquid ratio for achieving a maximum sulfated polysaccharide yield of 48.14% with an increased of 12.08% compared with that given by hot water extraction were 20 min,150 W and 1:90 (g/mL),respectively.The dried crude product obtained using the optimized ultrasonic-assisted extraction process exhibited polysaccharide and sulfate group contents of 96.94% and 19.66%,respectively.Neither protein nor nucleic acid was found in ESSP obtained using hot water extraction or this method,which contained characteristic peaks of polysaccharides and sulfate groups shown in IR spectrum.