CAJ | 학술논문

研究了牛蒡提取物对副溶血性弧菌的抑制作用.采用80％乙醇分别浸提两个牛蒡品种柳川理想及黄肌牛蒡根的皮及去皮根,四种提取物分别命名为LP、LR、HP和HR.采用滤纸片扩散和最低抑菌浓度法筛选了四种提取物的抑菌效果,时变抑菌研究了常温(25℃)和低温(4℃)下牛蒡提取物在无菌蒸馏水(DW)以及脑心浸汤(BHI)中对副溶血性弧菌的抑制效果.结果显示,四种提取物中LP的抑菌活性最好；经1000μL/mL LP提取液7小时处理,在4℃和25℃DW环境均未有副溶血性弧菌检出；经各浓度提取液1天处理,在4℃和25℃BHI环境均未有副溶血性弧菌检出.LP对副溶血性弧菌弧菌具有良好的抑制作用.
연구료우방제취물대부용혈성호균적억제작용.채용80％을순분별침제량개우방품충류천이상급황기우방근적피급거피근,사충제취물분별명명위LP、LR、HP화HR.채용려지편확산화최저억균농도법사선료사충제취물적억균효과,시변억균연구료상온(25℃)화저온(4℃)하우방제취물재무균증류수(DW)이급뇌심침탕(BHI)중대부용혈성호균적억제효과.결과현시,사충제취물중LP적억균활성최호；경1000μL/mL LP제취액7소시처리,재4℃화25℃DW배경균미유부용혈성호균검출；경각농도제취액1천처리,재4℃화25℃BHI배경균미유부용혈성호균검출.LP대부용혈성호균호균구유량호적억제작용.
The antibacterial activities of burdock roots against Vibrio parahemolyticus were investigated.The peel and peeled root of two varieties of burdock named Liuchuan and Huangji were extracted with 80％ ethanol,classed as LP,LR,HP and HR respectively.The data from disk diffusion method and minimum inhibitory concentration (MIC) values showed that the antibacterial activity of LP was more significant than that of LR,HP and HR.The time - kill study indicated that V.parahemolyticus was reduced to non-detectable levels with 1000 μL/mL LP extract treatment at 4 and 25 ℃ for 7 h in DW and no bacteria cell was detected with all treatment on Day 1 at 4 and 25 ℃ in BHI.Basing on its good antibacterial activitiy,LP could be useful in control of V.parahemolyticus.