CAJ | 학술논문

目的观察B组柯萨奇病毒感染对大鼠心肌来源的H9c2细胞microRNA表达谱的影响.方法用B组柯萨奇病毒3型Nancy株(CVB3)攻击成层和未成层的H9c2细胞,6h后收获总RNA,基因芯片检测microRNA表达谱.结果比对未成层与成层的H9c2细胞的microRNA表达谱,H9c2细胞成层后有54种microRNA表达显著下调,7种microRNA表达显著上调.与未感染病毒的未成层细胞比较,CVB3感染的未成层H9c2细胞有35种microRNA表达显著下调,9种microRNA表达显著上调.与成层H9c2细胞比较,CVB3感染成层H9c2细胞后,有20种microRNA的表达显著下调,16种microRNA的表达显著上调.结论细胞生长状态、CVB3攻击均显著改变了H9c2细胞microRNA表达谱.
목적 관찰B조가살기병독감염대대서심기래원적H9c2세포microRNA표체보적영향.방법 용B조가살기병독3형Nancy주(CVB3)공격성층화미성층적H9c2세포,6h후수획총RNA,기인심편검측microRNA표체보.결과 비대미성층여성층적H9c2세포적microRNA표체보,H9c2세포성층후유54충microRNA표체현저하조,7충microRNA표체현저상조.여미감염병독적미성층세포비교,CVB3감염적미성층H9c2세포유35충microRNA표체현저하조,9충microRNA표체현저상조.여성층H9c2세포비교,CVB3감염성층H9c2세포후,유20충microRNA적표체현저하조,16충microRNA적표체현저상조.결론 세포생장상태、CVB3공격균현저개변료H9c2세포microRNA표체보.
Objective To observe the microRNA expression differentiation of rat myooardium-derived cell H9c2 infected with coxsackievirus B. Methods Confluent and unconfluent H9c2 cells were attacked with cox-sackievirus B3 Nancy strain (CVB3). Six hour later the total RNAs were harvested and were applied to the mi-croRNA expression profiling analysis by microarray. Results The expressions of 54 microRNAs were down-regulated and 7 were up-regulated in confluent H9c2 cells compared with unconfluent H9c2 cells. The expres-sions of 35 microRNAs were down-regulated and 9 were up-regulated among the unconfluent H9c2 infected with CVB3 compared with unconfluent H9c2 cells without CVB3 infection. There were 20 microRNAs down-regula-ted and 16 up-regulated in confluent H9c2 infected with CVB3 compared with confluent H9c2 cells without CVB3 infection. Conclusion There are differentiations of microRNA .expression profiles of H9c2 at different cellular status and with coxsackievirus infection.