中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2012年
2期
143-146
,共4页
胡海澜%莫建文%杨进顺%白波
鬍海瀾%莫建文%楊進順%白波
호해란%막건문%양진순%백파
创伤和损伤%静脉血栓形成%基因%寡核苷酸序列分析
創傷和損傷%靜脈血栓形成%基因%寡覈苷痠序列分析
창상화손상%정맥혈전형성%기인%과핵감산서렬분석
Wounds and injuries%Venous thrombosis%Genes%Oligonucleotide array sequence analysis
目的 筛选创伤性深静脉血栓形成(DVT)消退关键基因,探讨这些基因在创伤性DVT消退中的意义. 方法 取70只SD大鼠,分为正常对照组(A组,10只)和模型组(60只),通过定量打击方法建立创伤性DVT动物模型.模型组再筛选出2个亚组:血栓形成组(B组,10只)和血栓消退组(C组,10只),采用Genechip Rat Genome 430 2.0基因表达谱芯片检测DVT发生、发展过程中创伤局部静脉内基因差异表达的变化,应用时间序列分析方法进行分析,筛选影响血栓消退实验表型变化的关键基因. 结果 创伤性DVT消退关键基因主要有胶原Ⅺ-α1(COL11-α1)、葡萄糖醛酸酶-B(GUs-β)、基质金属肽酶-12(MMP-12)、成纤维细胞激活蛋白(FAP)、C1q和肿瘤坏死因子相关蛋白-3(C1QTNF3_PREDICTED)、酸性磷酸酶-5(ACP-5)、GTP酶激活因子12 (TBC1D12_PREDICTED)、钙蛋白酶-6(CAPN-6)等基因.A、B、C组COL11-α1基因丰度表达值分别为0、2.5、1.2,GUS-β基因丰度表达值分别为0、1.5、0.7,MMP-12基因丰度表达值分别为0、3.9、1.6,FAP基因丰度表达值分别为0、1.8、0.5,C1QTNF3_PREDICTED基因丰度表达值分别为0、5.7、3.5,ACP-5基因丰度表达值分别为0、1.2、0.8,TBC1D12_PREDICTED基因丰度表达值分别为0、1.7、0.8,CAPN-6基因丰度表达值分别为0、4.5、2.9.结论 在创伤性DVT演化过程中,COL11-α1、GUS-β、MMP-12、FAP、C1QTNF3_PREDICTED、ACP-5、TBC1D12_PREDICTED、CAPN-6等基因与血栓消退关系密切,是促进血栓消退的关键基因.
目的 篩選創傷性深靜脈血栓形成(DVT)消退關鍵基因,探討這些基因在創傷性DVT消退中的意義. 方法 取70隻SD大鼠,分為正常對照組(A組,10隻)和模型組(60隻),通過定量打擊方法建立創傷性DVT動物模型.模型組再篩選齣2箇亞組:血栓形成組(B組,10隻)和血栓消退組(C組,10隻),採用Genechip Rat Genome 430 2.0基因錶達譜芯片檢測DVT髮生、髮展過程中創傷跼部靜脈內基因差異錶達的變化,應用時間序列分析方法進行分析,篩選影響血栓消退實驗錶型變化的關鍵基因. 結果 創傷性DVT消退關鍵基因主要有膠原Ⅺ-α1(COL11-α1)、葡萄糖醛痠酶-B(GUs-β)、基質金屬肽酶-12(MMP-12)、成纖維細胞激活蛋白(FAP)、C1q和腫瘤壞死因子相關蛋白-3(C1QTNF3_PREDICTED)、痠性燐痠酶-5(ACP-5)、GTP酶激活因子12 (TBC1D12_PREDICTED)、鈣蛋白酶-6(CAPN-6)等基因.A、B、C組COL11-α1基因豐度錶達值分彆為0、2.5、1.2,GUS-β基因豐度錶達值分彆為0、1.5、0.7,MMP-12基因豐度錶達值分彆為0、3.9、1.6,FAP基因豐度錶達值分彆為0、1.8、0.5,C1QTNF3_PREDICTED基因豐度錶達值分彆為0、5.7、3.5,ACP-5基因豐度錶達值分彆為0、1.2、0.8,TBC1D12_PREDICTED基因豐度錶達值分彆為0、1.7、0.8,CAPN-6基因豐度錶達值分彆為0、4.5、2.9.結論 在創傷性DVT縯化過程中,COL11-α1、GUS-β、MMP-12、FAP、C1QTNF3_PREDICTED、ACP-5、TBC1D12_PREDICTED、CAPN-6等基因與血栓消退關繫密切,是促進血栓消退的關鍵基因.
목적 사선창상성심정맥혈전형성(DVT)소퇴관건기인,탐토저사기인재창상성DVT소퇴중적의의. 방법 취70지SD대서,분위정상대조조(A조,10지)화모형조(60지),통과정량타격방법건립창상성DVT동물모형.모형조재사선출2개아조:혈전형성조(B조,10지)화혈전소퇴조(C조,10지),채용Genechip Rat Genome 430 2.0기인표체보심편검측DVT발생、발전과정중창상국부정맥내기인차이표체적변화,응용시간서렬분석방법진행분석,사선영향혈전소퇴실험표형변화적관건기인. 결과 창상성DVT소퇴관건기인주요유효원Ⅺ-α1(COL11-α1)、포도당철산매-B(GUs-β)、기질금속태매-12(MMP-12)、성섬유세포격활단백(FAP)、C1q화종류배사인자상관단백-3(C1QTNF3_PREDICTED)、산성린산매-5(ACP-5)、GTP매격활인자12 (TBC1D12_PREDICTED)、개단백매-6(CAPN-6)등기인.A、B、C조COL11-α1기인봉도표체치분별위0、2.5、1.2,GUS-β기인봉도표체치분별위0、1.5、0.7,MMP-12기인봉도표체치분별위0、3.9、1.6,FAP기인봉도표체치분별위0、1.8、0.5,C1QTNF3_PREDICTED기인봉도표체치분별위0、5.7、3.5,ACP-5기인봉도표체치분별위0、1.2、0.8,TBC1D12_PREDICTED기인봉도표체치분별위0、1.7、0.8,CAPN-6기인봉도표체치분별위0、4.5、2.9.결론 재창상성DVT연화과정중,COL11-α1、GUS-β、MMP-12、FAP、C1QTNF3_PREDICTED、ACP-5、TBC1D12_PREDICTED、CAPN-6등기인여혈전소퇴관계밀절,시촉진혈전소퇴적관건기인.
Objective To screen the key genes for thrombi resolution in a rat model of trauma deep vein thrombosis (DVT). Methods Seventy Sprague Dawley rats were divided into a normal control group (group A,10 rats) and a model group (60 rats).The traumatic DVT model was established by quantitative beating on the bilateral posterior limbs.From the model group a thrombosis group (group B,10 rats) and a thrombosis-resolution group (group C,10 rats) were screened.The Genechip Rat genome 430 2.0 was used to detect changes in gene expressions on difference phases of DVT after trauma.Time series analysis was used to screen the key genes that affected the phenotype changes associated with thrombi resolution. Results COL11-α1,GUS-β,MMP12,FAP,C1QTNF3_PREDICTED,ACP-5,TBC1D12_PREDICTED and CAPN-6 were screened as the key genes for thrombi resolution in traumatic DVT.In groups A,B and C,the abundance expressions of COL11-α1 were respectively 0,2.5 and 1.2; those of GUS-β were respectively 0,1.5 and 0.7; those of MMP-12 were respectively 0,3.9 and 1.6; those of FAP were respectively 0,1.8 and 0.5; those of C1QTNF3_PREDICTED were respectively 0,5.7 and 3.5; those of ACP-5 were respectively 0,1.2 and 0.8; those of TBC1D12_PREDICTED were respectively 0,1.7 and 0.8.; those of CAPN-6 were respectively 0,4.5 and 2.9.These genes had the same expression mode and coincided with the biological phenotype of thrombi. Conclusion In the process of traumatic DVT,as COL11-α1,GUS-β,MMP-12,FAP,C1QTNF3_PREDICTED,ACP-5,TBC1D12_PREDICTED and CAPN-6,have an intimate relation with the resolution of thrombi,they may be the key genes that promote thrombi resolution.
