中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2008年
9期
867-869
,共3页
表皮生长因子受体基因%骨肉瘤%荧光原位杂交技术%基因扩增
錶皮生長因子受體基因%骨肉瘤%熒光原位雜交技術%基因擴增
표피생장인자수체기인%골육류%형광원위잡교기술%기인확증
Epirnd growth factor receptor genes%Osteosarcoma%Fluorescence in situ hy- bridization%Gene amplification
目的 应用荧光原位杂交技术检测骨肉瘤人表皮生长因子2受体(HER-2/neu)基因是否存在扩增.方法 以HER-2/neu及17号染色体为探针,应用荧光原位杂交技术分析23例骨肉瘤病例HER-2/neu基因扩增情况,以HER-2/neu基因扩增阳性的乳腺癌病例作为阳性对照.结果 FISH检测23例骨肉瘤标本,无一例出现HER-2/neu基因扩增.结论 骨肉瘤中出现HER-2/neu基因扩增的频率极低,骨肉瘤患者可能不适合应用以HER-2/neu基因为靶点的靶向性药物.
目的 應用熒光原位雜交技術檢測骨肉瘤人錶皮生長因子2受體(HER-2/neu)基因是否存在擴增.方法 以HER-2/neu及17號染色體為探針,應用熒光原位雜交技術分析23例骨肉瘤病例HER-2/neu基因擴增情況,以HER-2/neu基因擴增暘性的乳腺癌病例作為暘性對照.結果 FISH檢測23例骨肉瘤標本,無一例齣現HER-2/neu基因擴增.結論 骨肉瘤中齣現HER-2/neu基因擴增的頻率極低,骨肉瘤患者可能不適閤應用以HER-2/neu基因為靶點的靶嚮性藥物.
목적 응용형광원위잡교기술검측골육류인표피생장인자2수체(HER-2/neu)기인시부존재확증.방법 이HER-2/neu급17호염색체위탐침,응용형광원위잡교기술분석23례골육류병례HER-2/neu기인확증정황,이HER-2/neu기인확증양성적유선암병례작위양성대조.결과 FISH검측23례골육류표본,무일례출현HER-2/neu기인확증.결론 골육류중출현HER-2/neu기인확증적빈솔겁저,골육류환자가능불괄합응용이HER-2/neu기인위파점적파향성약물.
Objective To investigate whether HER-2/neu oneogene amplification exists in 08- teosarcomas. Methods Fluorescence in situ hybridization(FISH)and DNA probes for HER-2/neu and centromere 17 were used to examine the HER-2/neu gene amplification in samples taken from 23 patients with osteosarcoma. Results None of the 23 osteosarcoma samples showed the HER-2/neu gene amplification.Condusion Since HER-2/neu gene amplification is rare in osteosarcomas, HER-2/neu target medicine should not be applied to patients with osteosarcoma
