中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2010年
6期
504-506
,共3页
可塑性相关基因-1%惊厥%大脑皮质%发育
可塑性相關基因-1%驚厥%大腦皮質%髮育
가소성상관기인-1%량궐%대뇌피질%발육
Plasticity-related gene 1%Recurrent seizures%Cerebral cortex%Developmental
目的 探索可塑性相关基因-1(plasticity related gene 1,PRG-1)在反复惊厥大鼠大脑皮质中的动态表达及其意义.方法 用三氟乙醚使新生第6天的SD大鼠呈现反复惊厥状态,每天持续30min,连续6d,分别于末次惊厥后3h,12h,48 h和14d取大脑皮质,并每个时间点设未做任何干预对照组,用免疫印迹方法观察PRG-1蛋白表达.结果 末次惊厥后3 h,12h,48 h、14d大脑皮质可塑性相关基因-1的表达,对照组分别为1.363±0.742、1.278±0.687、0.763±0.374、1.004±0.113,实验组分为1.818±1.093、1.562±0.782、1.024±0.510、1.378±0.279.3 h、12h、48 h大脑皮质PRG-1的表达在实验组和对照组无统计学意义(t=0.843,0.668,1.011,均P＞0.05).14d大脑皮质PRG-1的表达,实验组明显高于对照组(t=3.041,P＜0.05).结论 PRG-1在新生大鼠反复长程惊厥后的远期表达增多,提示参与发育期惊厥性脑损伤的病理生理机制.
目的 探索可塑性相關基因-1(plasticity related gene 1,PRG-1)在反複驚厥大鼠大腦皮質中的動態錶達及其意義.方法 用三氟乙醚使新生第6天的SD大鼠呈現反複驚厥狀態,每天持續30min,連續6d,分彆于末次驚厥後3h,12h,48 h和14d取大腦皮質,併每箇時間點設未做任何榦預對照組,用免疫印跡方法觀察PRG-1蛋白錶達.結果 末次驚厥後3 h,12h,48 h、14d大腦皮質可塑性相關基因-1的錶達,對照組分彆為1.363±0.742、1.278±0.687、0.763±0.374、1.004±0.113,實驗組分為1.818±1.093、1.562±0.782、1.024±0.510、1.378±0.279.3 h、12h、48 h大腦皮質PRG-1的錶達在實驗組和對照組無統計學意義(t=0.843,0.668,1.011,均P＞0.05).14d大腦皮質PRG-1的錶達,實驗組明顯高于對照組(t=3.041,P＜0.05).結論 PRG-1在新生大鼠反複長程驚厥後的遠期錶達增多,提示參與髮育期驚厥性腦損傷的病理生理機製.
목적 탐색가소성상관기인-1(plasticity related gene 1,PRG-1)재반복량궐대서대뇌피질중적동태표체급기의의.방법 용삼불을미사신생제6천적SD대서정현반복량궐상태,매천지속30min,련속6d,분별우말차량궐후3h,12h,48 h화14d취대뇌피질,병매개시간점설미주임하간예대조조,용면역인적방법관찰PRG-1단백표체.결과 말차량궐후3 h,12h,48 h、14d대뇌피질가소성상관기인-1적표체,대조조분별위1.363±0.742、1.278±0.687、0.763±0.374、1.004±0.113,실험조분위1.818±1.093、1.562±0.782、1.024±0.510、1.378±0.279.3 h、12h、48 h대뇌피질PRG-1적표체재실험조화대조조무통계학의의(t=0.843,0.668,1.011,균P＞0.05).14d대뇌피질PRG-1적표체,실험조명현고우대조조(t=3.041,P＜0.05).결론 PRG-1재신생대서반복장정량궐후적원기표체증다,제시삼여발육기량궐성뇌손상적병리생리궤제.
Objective To explore the dynamic expression of a new phospholipid phosphatase, plasticity related gene 1 ( PRG-1 ) in cerebral cortex following recurrent neonatal seizures.Methods A seizure was induced by inhalant flurothyl daily in neonatal Sprague-Dawley rats from postnatal day 6 (P6).Rats were assigned into the recurrent-seizure group (seizures induced in six consecutive days) and the control group.At 3 h,12 h,48 h,14d after the last convulsion,PRG-1 protein level in cerebral cortex was detected by western blot method.Results At 3 h, 12 h,48 h, 14d after the last convulsion in control group, the expression of PRG-1 in cerebral cortex respectively was 1.363 ± 0.742,1.278 ± 0.687 ,0.763 ± 0.374,1.004 ± 0.113, in experimental group , the expression of PRG-1 in cerebral cortex respectively was 1.818 ± 1.093,1.562 ± 0.782,1.024 ± 0.510,1.378 ± 0.279.At 3 h, 12 h,48 h, the expression of PRG-1 in cerebral cortex was not significantly different between the experimental and control group( t = 0.843,0.668,1.011 ,all P ＞ 0.05 ).However, at 14 d, the level of PRG-1 protein in cerebral cortex of experimental group was significantly higher than that of control ( t = 3.041, P ＜ 0.05 ).Conclusion The up-regulated expression of PRG-1 in cerebral cortex may be associated with the recurrent neonatal seizure-induced brain damage.
