中国医药
中國醫藥
중국의약
CHINA MEDICINE
2011年
7期
808-810
,共3页
吕华荣%王峻%吴星%戴学元%裴永恩%雷霆
呂華榮%王峻%吳星%戴學元%裴永恩%雷霆
려화영%왕준%오성%대학원%배영은%뢰정
多形性胶质母细胞瘤%苯妥英钠%多药耐药%逆转
多形性膠質母細胞瘤%苯妥英鈉%多藥耐藥%逆轉
다형성효질모세포류%분타영납%다약내약%역전
Glioblastoma muhiforme%Phenytoin%Multidrug resistance%Reverse
目的 研究不同浓度苯妥英钠对化疗耐药胶质母细胞瘤细胞系(8-MG-BA)化疗耐药逆转的影响.方法 用不同浓度苯妥英钠对8-MG-BA及H4细胞系处理后,以噻唑蓝检测其对上述2种细胞系化疗耐药逆转的影响,并与维拉帕米处理组进行对照.结果 用苯妥英钠5、10 mg/L和维拉帕米5 mg/L处理后,8-MG-BA细胞对卡莫司汀的逆转倍数分别为25.34、34.97和14.27,对替尼泊苷耐药的逆转倍数分别为13.44、21.71和11.10.结论 苯妥英钠是一种新型多药耐药逆转剂,逆转作用大于维拉帕米,且呈现剂量依赖关系.
目的 研究不同濃度苯妥英鈉對化療耐藥膠質母細胞瘤細胞繫(8-MG-BA)化療耐藥逆轉的影響.方法 用不同濃度苯妥英鈉對8-MG-BA及H4細胞繫處理後,以噻唑藍檢測其對上述2種細胞繫化療耐藥逆轉的影響,併與維拉帕米處理組進行對照.結果 用苯妥英鈉5、10 mg/L和維拉帕米5 mg/L處理後,8-MG-BA細胞對卡莫司汀的逆轉倍數分彆為25.34、34.97和14.27,對替尼泊苷耐藥的逆轉倍數分彆為13.44、21.71和11.10.結論 苯妥英鈉是一種新型多藥耐藥逆轉劑,逆轉作用大于維拉帕米,且呈現劑量依賴關繫.
목적 연구불동농도분타영납대화료내약효질모세포류세포계(8-MG-BA)화료내약역전적영향.방법 용불동농도분타영납대8-MG-BA급H4세포계처리후,이새서람검측기대상술2충세포계화료내약역전적영향,병여유랍파미처리조진행대조.결과 용분타영납5、10 mg/L화유랍파미5 mg/L처리후,8-MG-BA세포대잡막사정적역전배수분별위25.34、34.97화14.27,대체니박감내약적역전배수분별위13.44、21.71화11.10.결론 분타영납시일충신형다약내약역전제,역전작용대우유랍파미,차정현제량의뢰관계.
0bjective To investigate the reversal of chemotherapy resistance of glioblastoma cell line 8-MG-BA after incubated in different concentrations of phenytoin(PHT)in vitro.Methods After adding PHT on different concentrations,the reversal of chemotherapy resistance of carmustine and VM-26 on H4 and 8-MG-BA cell line was determined by thiazolyl blue tetrazolium bromide(MTr)assay.Verapamil was applied in a control group.Results With PHT 5mg/L or 10 mg/L,verapamil(VRP)5 mg/L,the fold of reversal of carmustine was 25.34,34.97,14.27 respectively,and that of VM-26 was 13.44,21.71,11.10 respectively.Conclusion Fold of reversal increases more with the dose of PHT that with VRP.
