中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2009年
3期
181-185
,共5页
董格红%王桂秋%宫丽平%王晋芬%莫祥兰%刘红刚%董丽娜%周英琼%张雪梅%冯振博%高子芬%叶洪涛
董格紅%王桂鞦%宮麗平%王晉芬%莫祥蘭%劉紅剛%董麗娜%週英瓊%張雪梅%馮振博%高子芬%葉洪濤
동격홍%왕계추%궁려평%왕진분%막상란%류홍강%동려나%주영경%장설매%풍진박%고자분%협홍도
淋巴瘤,B细胞,边缘区%原位杂交,荧光%染色体畸变
淋巴瘤,B細胞,邊緣區%原位雜交,熒光%染色體畸變
림파류,B세포,변연구%원위잡교,형광%염색체기변
Lymphoma,B-cell,marginal zone%In aitu hybridisation,fluorescence%Chromosome aberrations
目的 探讨中国人不同部位黏膜相关淋巴组织结外边缘区淋巴瘤(MALTL)中分子遗传学异常的发生情况.方法 应用间期荧光原位杂交(FISH)方法,检测217例不同部位MALTL的t(11;18)(q21;q21)/API2-MALT1、t(1;14)(p22;q32)/IGH-BCL10、t(14;18)(q32;q21)/IGH-MALT1和涉及BCL6基因的染色体易位.结果 染色体易位的总发生率为21%(46/217).全部病例共检测出4种主要染色体异常,其中第1种13%(29/217)为t(11;18)(q21;q21)/API2-MALT1,最常见的发生部位是肺47%(8/17)和小肠29%(4/14),其次为唾液腺1/6例、胃14%(12/84)和眼附属器6%(4/68).第2种1%(3/217)为t(1;14)(p22;q32)/IGH-BCL10,仪见于肺12%(2/17)和胃1%(1/84).第3种1%(2/217)为t(14;18)(q32;q21)/IGH-MALTI,仅见于肺6%(1/17)和眼附属器2%(1/68).第4种2%(4/217)为BCL6基因涉及的染色体易位,见于唾液腺1/6例和胃4%(3/84).4%(8/217)为涉及IGH基因但未知与其易位的伙伴基因的染色体易位.结论 以上4种染色体易位在中国人不同解剖部位的MALTL中的发生率有明显不同,与欧美国家所报道的染色体易位的发生率相比较,分布稍有差异.
目的 探討中國人不同部位黏膜相關淋巴組織結外邊緣區淋巴瘤(MALTL)中分子遺傳學異常的髮生情況.方法 應用間期熒光原位雜交(FISH)方法,檢測217例不同部位MALTL的t(11;18)(q21;q21)/API2-MALT1、t(1;14)(p22;q32)/IGH-BCL10、t(14;18)(q32;q21)/IGH-MALT1和涉及BCL6基因的染色體易位.結果 染色體易位的總髮生率為21%(46/217).全部病例共檢測齣4種主要染色體異常,其中第1種13%(29/217)為t(11;18)(q21;q21)/API2-MALT1,最常見的髮生部位是肺47%(8/17)和小腸29%(4/14),其次為唾液腺1/6例、胃14%(12/84)和眼附屬器6%(4/68).第2種1%(3/217)為t(1;14)(p22;q32)/IGH-BCL10,儀見于肺12%(2/17)和胃1%(1/84).第3種1%(2/217)為t(14;18)(q32;q21)/IGH-MALTI,僅見于肺6%(1/17)和眼附屬器2%(1/68).第4種2%(4/217)為BCL6基因涉及的染色體易位,見于唾液腺1/6例和胃4%(3/84).4%(8/217)為涉及IGH基因但未知與其易位的夥伴基因的染色體易位.結論 以上4種染色體易位在中國人不同解剖部位的MALTL中的髮生率有明顯不同,與歐美國傢所報道的染色體易位的髮生率相比較,分佈稍有差異.
목적 탐토중국인불동부위점막상관림파조직결외변연구림파류(MALTL)중분자유전학이상적발생정황.방법 응용간기형광원위잡교(FISH)방법,검측217례불동부위MALTL적t(11;18)(q21;q21)/API2-MALT1、t(1;14)(p22;q32)/IGH-BCL10、t(14;18)(q32;q21)/IGH-MALT1화섭급BCL6기인적염색체역위.결과 염색체역위적총발생솔위21%(46/217).전부병례공검측출4충주요염색체이상,기중제1충13%(29/217)위t(11;18)(q21;q21)/API2-MALT1,최상견적발생부위시폐47%(8/17)화소장29%(4/14),기차위타액선1/6례、위14%(12/84)화안부속기6%(4/68).제2충1%(3/217)위t(1;14)(p22;q32)/IGH-BCL10,의견우폐12%(2/17)화위1%(1/84).제3충1%(2/217)위t(14;18)(q32;q21)/IGH-MALTI,부견우폐6%(1/17)화안부속기2%(1/68).제4충2%(4/217)위BCL6기인섭급적염색체역위,견우타액선1/6례화위4%(3/84).4%(8/217)위섭급IGH기인단미지여기역위적화반기인적염색체역위.결론 이상4충염색체역위재중국인불동해부부위적MALTL중적발생솔유명현불동,여구미국가소보도적염색체역위적발생솔상비교,분포초유차이.
Objective To investigate the genetic aberrations in extranodal marginal zone lymphoma of mueosa-associated lymphoid tissue (MALT) lymphomas from different sites of the body in Chinese patients. Methods Two hundred and seventeen paraffin-embedded MALT lymphoma specimens from 11 major sites were studied with interphase fluorescence in situ hybridization (FISH) to detect t(11; 18) (q21;q21)/API2-MALT1, t(1; 14) (p22; q32)/IGH-BCL10, (14; 18) (q32; q21)/IGH-MALT1 and BCL6 gene involved chromosome translocations. Results These translocations were mutually exclusive and detected in 21% (46/217) of the cases, including t(11;18) (q21;q21) API2-MALT1 13% (29/217), t (1;14)(p22 ;q32) IGH-BCLIO in 1% (3/217), t(14;18) (q32;q21) IGH-MALT1 1% (2/217), BCL6 involved translocation in 2% (4/217) and IGH-unknown translocation partner in 4% (8/217). t(11; 18) (q21;q21)API2-MALT1 was found with the highest frequency in MALT lymphoma from lungs (47% , 8/17) and small intestine (29%, 4/14), followed by salivary gland (17%, 1/6), stomach (14%, 12/84) and ocular adnexae (6% , 4/68). t(1 ;14) (p22;q32) was only detected in lungs (12%, 2/17) and stomach (1%, 1/84). t(14;18) (q32;q21) was mainly detected in lungs (6%, 1/17) and ocular adnexae (2%, 1/68). BCL6 gene involved translocation was detected in salivary gland (17% , 1/6) and stomach (4%, 3/84). Conclusions It is demonstrated that the four translocatidns occur with markedly variable frequencies in MALT lymphoma of different sites in Chinese patients. The distributions of these chromosome translocations in Chinese patients are slightly different from those reported in western patients.
