CAJ | 학술논문

目的观察辛伐他汀对兔髓核细胞Ⅱ型胶原(ColⅡ)及聚集蛋白聚糖(Agg)表达的影响.方法取兔髓核细胞进行原代培养,传至第3代行ColⅡ免疫组织化学鉴定后随机分为5组,以不同浓度辛伐他汀处理:A组:空白对照组;B、C、D、E组分别为0.1、0.2、0.4、0.8 μmol/L辛伐他汀组.运用半定量逆转录-聚合酶链反应(RT-PCR)检测ColⅡ、Agg含量的变化,并行细胞活力检测.结果辛伐他汀浓度超过0.2 μmol/L时ColⅡ及Agg的表达增加,0.4 μmoL/L时达到高峰(P＜0.05),0.8 μmol/L时ColⅡ及Agg表达下降.0.8 μmol/L处理组影响细胞活力,0.1～0.4 μmol/L范围时细胞活性无明显影响(P＜0.05).结论辛伐他汀可促进兔髓核细胞ColⅡ及Agg的表达,改善椎间盘退变进程;在＜0.4 μmol/L的较低浓度内对细胞活力无明显影响.
목적 관찰신벌타정대토수핵세포Ⅱ형효원(ColⅡ)급취집단백취당(Agg)표체적영향.방법 취토수핵세포진행원대배양,전지제3대행ColⅡ면역조직화학감정후수궤분위5조,이불동농도신벌타정처리:A조:공백대조조;B、C、D、E조분별위0.1、0.2、0.4、0.8 μmol/L신벌타정조.운용반정량역전록-취합매련반응(RT-PCR)검측ColⅡ、Agg함량적변화,병행세포활력검측.결과 신벌타정농도초과0.2 μmol/L시ColⅡ급Agg적표체증가,0.4 μmoL/L시체도고봉(P＜0.05),0.8 μmol/L시ColⅡ급Agg표체하강.0.8 μmol/L처리조영향세포활력,0.1～0.4 μmol/L범위시세포활성무명현영향(P＜0.05).결론 신벌타정가촉진토수핵세포ColⅡ급Agg적표체,개선추간반퇴변진정;재＜0.4 μmol/L적교저농도내대세포활력무명현영향.
Objective To investigate the expression of type Ⅱ collagen and aggrecan in rat nucleus pulposus (NP) cells exposed to various concentrations of simvastatin. Methods NP cells from a rabbit disc were harvested and cultured in vitro, and type Ⅱ collagen from the third generation of cells was identified by immunohistochemical method. The NP cells were randomly divided into five groups: A, blank group; B, 0. 1 μmol/L simvastatin; C, 0. 2 μmol/L simvastatin; D, 0.4 μmol/L simvastatin and E, 0. 8μmol/L simvastatin. Semi-quantitative real-time polymerase chain reaction (RT-PCR) was used to quantify type Ⅱ collagen and aggrecan. The changes of cells viability were observed. Results Simvastatin could promote type Ⅱ collagen and aggrecan expression at the concentration of exceeding 0. 2 μmol/L, and the expression reached the peak at 0. 4μmol/L, while descended at 0. 8 μmoL/L. Simvastatin at 0. 8 μmol/L affected the viability of the NP cells exposed, but simvastatin at the concentration of 0. 1 to 0. 4 μmol/Ldidn' t obviously affect cell activity. Conclusion Simvastatin can promote type Ⅱ collagen and aggrecan expression in rabbit NP cells, and slow down the process of disc degeneration. Within the concentration range less than 0. 4 μmol/L, simvastatin has no obvisou effects on the cell viability.