中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2010年
9期
790-793
,共4页
杨帆%林松%黄炎兰%伍伟锋
楊帆%林鬆%黃炎蘭%伍偉鋒
양범%림송%황염란%오위봉
心肌炎%肠道病毒属%T淋巴细胞,辅助诱导
心肌炎%腸道病毒屬%T淋巴細胞,輔助誘導
심기염%장도병독속%T림파세포,보조유도
Myocarditis%Enterovirus%T-lymphocytes,helper-inducer
目的 观察病毒性心肌炎小鼠不同时间点辅助性T淋巴细胞17(Th17)亚群的变化,探讨其在病毒性心肌炎发病中的作用.方法 用Balb/c小鼠建立柯萨奇病毒B3(CVB3)心肌炎小鼠模型(实验组),对照组注射等量磷酸盐缓冲液,在注射的第0、7、14、21、28和42天苏木精-伊红染色观察心肌病理改变并计算心肌病理积分,流式细胞仪检测小鼠脾脏Th17细胞亚群的比例(Th17/CD4+).结果 实验组7 d亚组小鼠心肌组织病理积分(1.8±0.5)高于0 d亚组,14 d亚组病理积分在实验组各亚组中最高(2.8±0.4),从21 d亚组起病理积分开始低于14 d亚组,实验组各亚组与对照组相应时点亚组比较差异均有统计学意义(P均<0.05).实验组7 d亚组小鼠脾Th17/CD4+[(2.23±0.89)%]高于实验组0 d亚组,28 d亚组脾Th17/CD4+最高[(5.00±0.81)%],42 d亚组脾Th17/CD4+[(2.35±0.35)%]低于实验组28 d亚组,与对照组相应时点亚组比较差异均有统计学意义(P均<0.05).结论 病毒性心肌炎小鼠中存在Th17细胞,其可能参与了病毒性心肌炎的炎性过程.
目的 觀察病毒性心肌炎小鼠不同時間點輔助性T淋巴細胞17(Th17)亞群的變化,探討其在病毒性心肌炎髮病中的作用.方法 用Balb/c小鼠建立柯薩奇病毒B3(CVB3)心肌炎小鼠模型(實驗組),對照組註射等量燐痠鹽緩遲液,在註射的第0、7、14、21、28和42天囌木精-伊紅染色觀察心肌病理改變併計算心肌病理積分,流式細胞儀檢測小鼠脾髒Th17細胞亞群的比例(Th17/CD4+).結果 實驗組7 d亞組小鼠心肌組織病理積分(1.8±0.5)高于0 d亞組,14 d亞組病理積分在實驗組各亞組中最高(2.8±0.4),從21 d亞組起病理積分開始低于14 d亞組,實驗組各亞組與對照組相應時點亞組比較差異均有統計學意義(P均<0.05).實驗組7 d亞組小鼠脾Th17/CD4+[(2.23±0.89)%]高于實驗組0 d亞組,28 d亞組脾Th17/CD4+最高[(5.00±0.81)%],42 d亞組脾Th17/CD4+[(2.35±0.35)%]低于實驗組28 d亞組,與對照組相應時點亞組比較差異均有統計學意義(P均<0.05).結論 病毒性心肌炎小鼠中存在Th17細胞,其可能參與瞭病毒性心肌炎的炎性過程.
목적 관찰병독성심기염소서불동시간점보조성T림파세포17(Th17)아군적변화,탐토기재병독성심기염발병중적작용.방법 용Balb/c소서건립가살기병독B3(CVB3)심기염소서모형(실험조),대조조주사등량린산염완충액,재주사적제0、7、14、21、28화42천소목정-이홍염색관찰심기병리개변병계산심기병리적분,류식세포의검측소서비장Th17세포아군적비례(Th17/CD4+).결과 실험조7 d아조소서심기조직병리적분(1.8±0.5)고우0 d아조,14 d아조병리적분재실험조각아조중최고(2.8±0.4),종21 d아조기병리적분개시저우14 d아조,실험조각아조여대조조상응시점아조비교차이균유통계학의의(P균<0.05).실험조7 d아조소서비Th17/CD4+[(2.23±0.89)%]고우실험조0 d아조,28 d아조비Th17/CD4+최고[(5.00±0.81)%],42 d아조비Th17/CD4+[(2.35±0.35)%]저우실험조28 d아조,여대조조상응시점아조비교차이균유통계학의의(P균<0.05).결론 병독성심기염소서중존재Th17세포,기가능삼여료병독성심기염적염성과정.
Objective To observe the alteration of T helper cells 17 (Th17) in mice with acute viral myocarditis (VMC) induced by coxsackie virus B3 (CVB3), explore the role of Th17 in mice VMC.Methods CVB3 or PBS was peritoneally injected to Balb/c male mice. Pathological scores were determined in hematoxylin-eosin stained sections and flow cytometric analysis was used to evaluate the frequencies of Th17 subsets in CD4+ T cells on 7, 14,21,28 and 42 days after virus injection. Results There were significant difference of the pathological scores between the VMC mice and the control ones ( P < 0. 05 ). The pathological scores of 7 d VMC subgroup were higher( 1.8 ± 0. 5) than those of O d VMC subgroup, and the scores of 14 d subgroup were highest (2. 8 ±0. 4) among the six subgroup of VMC mice, and then showed a decline tendency from 21 d group. Statistical difference of the proportion of Th17 cells were seen between the VMC and controls on different time points ( P < 0. 05 ). When compared with the 0 d VMC subgroup the proportion of spleen Th17 cells increased in 7 d VMC subgroup [(2. 23 ±0. 89)%], and peaked on 28 d [(5.00 ±0.81)%]. The results of Th17 proportion were lower than those of the 28 d subgroup.Conclusions Our data show that differentiated Th17 cells might be involved in the inflammation process of CVB3 induced VMC in mice.
