CAJ | 학술논문

目的探讨神经生长因子(NGF)经鼻腔给药对有机磷酸酯类化合物(OP)中毒大鼠急性脑损伤的影响和可能机制.方法建立SD大鼠OP中毒模型,常规治疗后进行症状学评分,将评分在2～3分的大鼠按随机数字表法分为阳性对照组和经鼻给予NGF组(n=6),另设正常对照组(n=6)在相应时间点给予生理盐水.中毒24 h后采用HE染色观察大鼠纹状体区神经细胞变性、坏死的情况,并测定纹状体区乙酰胆碱酯酶活性、丙二醛和还原型谷胱甘肽含量.结果 HE染色发现经鼻给予NGF可以减轻中毒后大鼠纹状体区神经细胞坏死程度.同时中毒后,经鼻给予NGF组和生理盐水组大鼠纹状体区乙酰胆碱酯酶活性均有明显下降[(0.46±0.11)比(0.35±0.09) U/mg prot],两组间比较差异无统计学意义(P＞0.05).但是与经鼻给予生理盐水组相比,NGF鼻腔给药的大鼠纹状体区丙二醛含量降低了25.14％[(4.0±0.9)比(5.4±1.3)nmol/mg prot],还原型谷胱甘肽水平增高了15.73％[(52.8±2.8)比(45.6±4.9)mg/g prot],两组间差异有统计学意义(P＜0.05).结论 NGF经鼻腔给药可以减轻由OP中毒后大鼠纹状体区损伤程度,其机制可能与对抗自由基损伤及脂质过氧化反应有关.
목적 탐토신경생장인자(NGF)경비강급약대유궤린산지류화합물(OP)중독대서급성뇌손상적영향화가능궤제.방법 건립SD대서OP중독모형,상규치료후진행증상학평분,장평분재2～3분적대서안수궤수자표법분위양성대조조화경비급여NGF조(n=6),령설정상대조조(n=6)재상응시간점급여생리염수.중독24 h후채용HE염색관찰대서문상체구신경세포변성、배사적정황,병측정문상체구을선담감지매활성、병이철화환원형곡광감태함량.결과 HE염색발현경비급여NGF가이감경중독후대서문상체구신경세포배사정도.동시중독후,경비급여NGF조화생리염수조대서문상체구을선담감지매활성균유명현하강[(0.46±0.11)비(0.35±0.09) U/mg prot],량조간비교차이무통계학의의(P＞0.05).단시여경비급여생리염수조상비,NGF비강급약적대서문상체구병이철함량강저료25.14％[(4.0±0.9)비(5.4±1.3)nmol/mg prot],환원형곡광감태수평증고료15.73％[(52.8±2.8)비(45.6±4.9)mg/g prot],량조간차이유통계학의의(P＜0.05).결론 NGF경비강급약가이감경유OP중독후대서문상체구손상정도,기궤제가능여대항자유기손상급지질과양화반응유관.
Objective To explore the protective effects of intranasal (IN) dosing of nerve growth factor (NGF) on brain injury induced by organophosphorus compounds (OP) in rats.Methods The OPtreated Sprague-Dawley rats received an intraperitoneal injection of atropine sulphate and pralidoxime at I min after intoxication.Then NGF or saline was dosed via the olfaetory pathway.All rats were sacrificed 24 hours after OP exposure.Damaged nerve cells were estimated on corpus striatum strained with hematoxylineosin (H&E) method.And the activity of acetylcholinesterase (AchE) and the eoncentrations of malondialdehyde (MIA) and reduced glutathione hormone (GSH) in corpus striatum were measured by colorimetric method.Results As assessed by H&E staining,a large number of degenerated and necrotic nerve cells were observed in corpus striatum in rats from in IN saline group.But in IN NGF group,the number of degenerated neurons was smaller than in IN NS group.Following OP exposure,the activity of AchE decreased in corpus striatum in both IN saline and IN NGF groups (0.46 ± 0.11 vs 0.35 ± 0.09 U/mg prot).No significant differences existed between two groups.But the concentrations of MDA in corpus striatum of IN NGF group rats reduced markedly by 25.14％ (4.02 ±0.85 vs 5.37 ± 1.33 nmol/mg prot) and the level of GSH increased sharply by 15.73％ (52.82 ± 2.80 vs 45.64 ± 4.88 mg/g prot) as compared with IN saline group (P ＜ 0.05).Conclusion Intranasal dosing of NGF may improve neuropathology and protect rats against OP-indueed oxidative damage in corpus striatum.