CAJ | 학술논문

目的揭示新疆库蚊分离的0507JS11病毒的基本特征,明确其分类地位.方法对0507JS11病毒进行细胞感染特点观察、负染电子显微镜(简称电镜)观察、基因组核酸电泳检测、全基因序列测定和系统进化分析.结果 0507JS11病毒可以引起Aedes albopictus C6/36细胞病变;完整病毒颗粒呈20面立体对称,直径20 nm,无包膜;病毒基因组为长3977 nt的单股正链DNA,基因组核酸电泳检测呈大小约4 kbp的DNA条带;病毒基因组编码区包括3个开放读码框(open readingframe,ORF),ORF1和ORF2编码非结构蛋白(non-structural protein,NS),ORF3编码衣壳蛋白(capsidprotein,VP);全基因序列系统进化分析显示病毒位于Brevidensovirus内一个独立进化分支.结论新疆库蚊分离的0507JS11病毒为Brevidensovirus新成员.
목적 게시신강고문분리적0507JS11병독적기본특정,명학기분류지위.방법 대0507JS11병독진행세포감염특점관찰、부염전자현미경(간칭전경)관찰、기인조핵산전영검측、전기인서렬측정화계통진화분석.결과 0507JS11병독가이인기Aedes albopictus C6/36세포병변;완정병독과립정20면입체대칭,직경20 nm,무포막;병독기인조위장3977 nt적단고정련DNA,기인조핵산전영검측정대소약4 kbp적DNA조대;병독기인조편마구포괄3개개방독마광(open readingframe,ORF),ORF1화ORF2편마비결구단백(non-structural protein,NS),ORF3편마의각단백(capsidprotein,VP);전기인서렬계통진화분석현시병독위우Brevidensovirus내일개독립진화분지.결론 신강고문분리적0507JS11병독위Brevidensovirus신성원.
Objective To probe the primary characteristic of 0507JS11 virus isolated from Culex sp. and determine the classification of 0507JS11 virus in taxonomy. Methods 0507JS11 virus was cultured in Aedes albopictus C6/36 cells and cytopathic effects (CPEs) were recorded. Electro-microscopic morphology of 0507JS11 virus was observed. Total DNA extract of 0507JS11 virus was detected by 1% Agarose Gel Electrophoresis. Complete genomic sequence of 0507JS11 virus was sequenced and then made phylogenetie analysis. Results 0507JS11 virus could cause CPEs in Aedes albopictus C6/36 cells. Viral particles have no envelope and appear icosahedron symmetry with diameter of 20 nm. The genome of 0507JS11 virus was positive single strand DNA (ssDNA) with full length of 3977 nt. However, a DNA band about 4 kbp was observed in the electrophoresis of total DNA extract of 0507JS11 virus. The coding region of the genome included three ORFs, ORF1 and ORF2 code NSP1 and NSP2, ORF3 codes VP. Phylogenetic analysis of the complete genomic sequence of 0507JS11 virus indicated an independent linear in Brevidensovirus. Conclusion 0507JS11 virus is a new member in Brevidensovirus.