农业工程学报
農業工程學報
농업공정학보
2003年
5期
176-181
,共6页
脱脂菜籽饼粕%酶解%菜籽清蛋白%碱性蛋白酶(alcalase)%水解度%响应曲面法
脫脂菜籽餅粕%酶解%菜籽清蛋白%堿性蛋白酶(alcalase)%水解度%響應麯麵法
탈지채자병박%매해%채자청단백%감성단백매(alcalase)%수해도%향응곡면법
defatted rapeseed meal%enzymatic hydrolysis%rapeseed albumin%alcalase%degree of hydrolysis%response surface methodology (RSM)
采用响应曲面法对碱性蛋白酶(alcalase)水解菜籽清蛋白工艺进行系统地研究.确定最佳水解条件如下:温度50℃、酶浓度0.38 AU/g、底物浓度4.87%.同时,葡聚糖凝胶(Sephadex G-25)柱层析显示水解物较原清蛋白分子量变小.氨基酸组成分析结果表明菜籽清蛋白水解物可作为一种营养丰富的食品添加剂加以广泛利用.
採用響應麯麵法對堿性蛋白酶(alcalase)水解菜籽清蛋白工藝進行繫統地研究.確定最佳水解條件如下:溫度50℃、酶濃度0.38 AU/g、底物濃度4.87%.同時,葡聚糖凝膠(Sephadex G-25)柱層析顯示水解物較原清蛋白分子量變小.氨基痠組成分析結果錶明菜籽清蛋白水解物可作為一種營養豐富的食品添加劑加以廣汎利用.
채용향응곡면법대감성단백매(alcalase)수해채자청단백공예진행계통지연구.학정최가수해조건여하:온도50℃、매농도0.38 AU/g、저물농도4.87%.동시,포취당응효(Sephadex G-25)주층석현시수해물교원청단백분자량변소.안기산조성분석결과표명채자청단백수해물가작위일충영양봉부적식품첨가제가이엄범이용.
The limited hydrolysis process for rapeseed albumin (RSA) with alcalase was systematically studied through response surface methodology (RSM). The optimum conditions were established, which included hydrolyzing temperature (50℃), enzyme concentration (0.38 Anson Units per gram of substrate) and concentration of substrate (4.87%). The gel filtration chromatography (Sephadex G-25) profile showed the major albumin protein was degraded after hydrolysis. In addition, the amino acid profiles indicated that hydrolyzed rapeseed albumin could be used as an additive with great potential in food industry.
