CAJ | 학술논문

[目的]筛选纤维素酶活性高且酶活稳定的纤维素降解菌.[方法]以木霉为出发菌株,用紫外线对其进行诱变处理,通过平板初筛和摇瓶复筛筛选出纤维素酶活力高且酶活稳定的优良突变株,然后分别以滤纸、甘蔗渣、麦秆和稻壳为目标降解物对优良突变株进行底物降解试验.[结果]经紫外线诱变、初筛、复筛,共选育出3株纤维素酶活力高且酶活稳定的优良突变株N_1、N_2、N_3,其中N_3的产酶活力最高(348 U/ml),为出发菌株的2.13倍;N_3对各种底物的降解效果均优于出发菌株,分别以滤纸、甘蔗渣、麦秆和稻壳为唯一碳源时,其失重率分别为48.68%、41.28%、26.53%和23.47%.[结论]该研究选育出了高效降解纤维素的菌株N_3.
[목적]사선섬유소매활성고차매활은정적섬유소강해균.[방법]이목매위출발균주,용자외선대기진행유변처리,통과평판초사화요병복사사선출섬유소매활력고차매활은정적우량돌변주,연후분별이려지、감자사、맥간화도각위목표강해물대우량돌변주진행저물강해시험.[결과]경자외선유변、초사、복사,공선육출3주섬유소매활력고차매활은정적우량돌변주N_1、N_2、N_3,기중N_3적산매활력최고(348 U/ml),위출발균주적2.13배;N_3대각충저물적강해효과균우우출발균주,분별이려지、감자사、맥간화도각위유일탄원시,기실중솔분별위48.68%、41.28%、26.53%화23.47%.[결론]해연구선육출료고효강해섬유소적균주N_3.
[Objective] The study was to screen the cellulose-decomposing microorganism with high and stable cellulase activity. [Method] With Trichoderma spp. As the original strain,it was mutagenized by ultraviolet,and the fine mutant with high and stable cellulase activity was screened out through primary and secondary screening. And then the substrate decomposition test of the fine mutant was conducted with filter paper,bagasse,wheat straw and rice husk as target degradation matter resp.. [Result] After ultraviolet mutation,primary and secondary screening,3 fine mutants with high and stable cellulase activity (N_1,N_2,N_3) were screened out,and among them the enzymatic activity of N_3 was the highest(348 U/ml),being 2.13 times of that of the original strain. The degradation effect of N_3 on various substrates was better than that of the original strain. When filter paper,bagasse,wheat straw and rice husk was taken as the sole carbon source resp.,their weight loss rates were 48.68%,41.28%,26.53% and 23.47% resp. [Conclusion] The study screened out the highly efficient cellulose-decomposing microorganism N_3.