CAJ | 학술논문

背景:球囊扩张及支架置入后再狭窄、管腔丢失等问题制约了支架置入治疗的进一步发展,血管内膜增殖和凋亡在再狭窄中的作用及干预方法尚在积极探索中.目的:采用Medtronic球囊建立大鼠颈动脉球囊损伤模型,观察瑞舒伐他汀对球囊损伤大鼠颈动脉平滑肌细胞增殖及凋亡的影响.方法:将雄性SD大鼠随机数字表法分为球囊损伤组和治疗组.均采用Medtronic球囊建立大鼠颈动脉球囊损伤模型,并取球囊损伤组大鼠的右侧颈总动脉(未行球囊损伤)作为正常对照.治疗组于球囊损伤前3 d始连续给予瑞舒伐他汀5 mg/(kg·d)灌胃,球囊损伤组给予9 g/L氯化钠溶液灌胃.术后7,14 d取颈总动脉进行苏木精-伊红染色,免疫组织化学检测SM α-actin、增殖细胞核抗原,采用TUNEL法检测平滑肌细胞凋亡情况.结果与结论:与球囊损伤组比较,治疗组造模后14 d,损伤血管新生内膜面积、新生内膜,中膜面积比值明显减少(P<0.05),管腔面积增加26%;增殖细胞核抗原阳性细胞率降低,凋亡阳性细胞率增高(P<o.05).提示支架置入球囊损伤前给予瑞舒伐他汀可抑制大鼠颈动脉球囊损伤后新生内膜增生及新生内膜细胞的增殖,促进平滑肌细胞的凋亡,从而减少动脉新生内膜形成,抑制再狭窄.
배경:구낭확장급지가치입후재협착、관강주실등문제제약료지가치입치료적진일보발전,혈관내막증식화조망재재협착중적작용급간예방법상재적겁탐색중.목적:채용Medtronic구낭건립대서경동맥구낭손상모형,관찰서서벌타정대구낭손상대서경동맥평활기세포증식급조망적영향.방법:장웅성SD대서수궤수자표법분위구낭손상조화치료조.균채용Medtronic구낭건립대서경동맥구낭손상모형,병취구낭손상조대서적우측경총동맥(미행구낭손상)작위정상대조.치료조우구낭손상전3 d시련속급여서서벌타정5 mg/(kg·d)관위,구낭손상조급여9 g/L록화납용액관위.술후7,14 d취경총동맥진행소목정-이홍염색,면역조직화학검측SM α-actin、증식세포핵항원,채용TUNEL법검측평활기세포조망정황.결과여결론:여구낭손상조비교,치료조조모후14 d,손상혈관신생내막면적、신생내막,중막면적비치명현감소(P<0.05),관강면적증가26%;증식세포핵항원양성세포솔강저,조망양성세포솔증고(P<o.05).제시지가치입구낭손상전급여서서벌타정가억제대서경동맥구낭손상후신생내막증생급신생내막세포적증식,촉진평활기세포적조망,종이감소동맥신생내막형성,억제재협착.
BACKGROUND:Restenosis and lumina loss limit further application of balloon extension and stent implantation.Effect of tunica intima proliferation and apoptosis in restenosis and the intervention method are exploring.OBJECTIVE:To investigate the influence of Rosuvastatin on the vascular smooth muscle cells proliferation and apoptosis in rats with carotid artery injury established by Medtronic balloon.METHODS:The male SD rats were randomly and equally divided into injury group and treatment group.Each rat was subjected to balloon injury on the lift common carotid artery,and control artery without balloon injury on the right artery served as control group.Treatment group rats were given Rosuvastatin(dissolved in Nacl)5 mg/kg per day 3 days before injury,while the injury group rats were given 9 g/L NaCl.At 7 and 14 days after injury,the common carotid arteries were harvested for HE staining.SM α-actin and proliferating celI nuclear antigen were detected by immunohistochemistry.In addition,smooth muscle cells apoptosis was detected by TUNEL.RESULTS AND CONCLUSION:The neointimal area and the area ratio of neointimal/media were decreased in treatment grouP significantly at 14 days compared with injury group(P<0.05),and neointimal area increased by 26%:positive cell rate of proliferating cell nuclear antigen was decreased,but apoptosis cells were increased cornpared with the injury group(P<0.05).Results showed that Rosuvastatin prior to balloon injury inhibited neointimal proliferation and neointimal celI proliferation following balloon Injury,promoted smooth muscle cells apoptosis,ultimately reducing neointimaI formation and inhibiting restenosis.