国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2012年
3期
236-241
,共6页
朱鹏飞%赵勇华%李晶媛%李晗%李树臣
硃鵬飛%趙勇華%李晶媛%李晗%李樹臣
주붕비%조용화%리정원%리함%리수신
基因芯片%PD-1/PD-L%慢性乙型肝炎
基因芯片%PD-1/PD-L%慢性乙型肝炎
기인심편%PD-1/PD-L%만성을형간염
Gene chip%PD-1/PD-L%Chronic hepatitis B
目的 应用基因芯片技术筛选慢乙肝病人PD-1/PD-L通路差异性表达的基因.方法 应用基因芯片技术筛选4例慢乙肝病人与2例健康人外周血,获得差异性表达的基因,结合初步构建的PD-1/PD-L信号通路,挑选出一致的转录因子或调控蛋白,并应用Western blot方法检测CD 40L、Bcl-10、GATA-3、NFAT蛋白的水平的表达.结果 与健康人相比,慢乙肝患者有838个异常表达的基因,其中高表达的基因有150个,低表达的基因有688个.在这些异常表达基因中,有13个基因属于所构建的PD-1/PD-L信号通路中的转录因子或调控蛋白,其中表达上调的6个分别是BCL-10、AP、SRY、MYOD1、ELK4、NFAT,表达下调的7个分别是CD 40L、GADS、PDK1、IL-4、GATA3、CREB、RARG.Westem blotting法证实慢乙肝组高表达BCL-10,低表达CD 40L、GATA3,NFAT但与病毒载量不相关.结论 CD40L、BCL-10、GATA3、NFAT在慢性乙型肝炎患者PD-1/PD-L信号传导通路中可能有重要的调控作用,可以做深入的功能分析研究.
目的 應用基因芯片技術篩選慢乙肝病人PD-1/PD-L通路差異性錶達的基因.方法 應用基因芯片技術篩選4例慢乙肝病人與2例健康人外週血,穫得差異性錶達的基因,結閤初步構建的PD-1/PD-L信號通路,挑選齣一緻的轉錄因子或調控蛋白,併應用Western blot方法檢測CD 40L、Bcl-10、GATA-3、NFAT蛋白的水平的錶達.結果 與健康人相比,慢乙肝患者有838箇異常錶達的基因,其中高錶達的基因有150箇,低錶達的基因有688箇.在這些異常錶達基因中,有13箇基因屬于所構建的PD-1/PD-L信號通路中的轉錄因子或調控蛋白,其中錶達上調的6箇分彆是BCL-10、AP、SRY、MYOD1、ELK4、NFAT,錶達下調的7箇分彆是CD 40L、GADS、PDK1、IL-4、GATA3、CREB、RARG.Westem blotting法證實慢乙肝組高錶達BCL-10,低錶達CD 40L、GATA3,NFAT但與病毒載量不相關.結論 CD40L、BCL-10、GATA3、NFAT在慢性乙型肝炎患者PD-1/PD-L信號傳導通路中可能有重要的調控作用,可以做深入的功能分析研究.
목적 응용기인심편기술사선만을간병인PD-1/PD-L통로차이성표체적기인.방법 응용기인심편기술사선4례만을간병인여2례건강인외주혈,획득차이성표체적기인,결합초보구건적PD-1/PD-L신호통로,도선출일치적전록인자혹조공단백,병응용Western blot방법검측CD 40L、Bcl-10、GATA-3、NFAT단백적수평적표체.결과 여건강인상비,만을간환자유838개이상표체적기인,기중고표체적기인유150개,저표체적기인유688개.재저사이상표체기인중,유13개기인속우소구건적PD-1/PD-L신호통로중적전록인자혹조공단백,기중표체상조적6개분별시BCL-10、AP、SRY、MYOD1、ELK4、NFAT,표체하조적7개분별시CD 40L、GADS、PDK1、IL-4、GATA3、CREB、RARG.Westem blotting법증실만을간조고표체BCL-10,저표체CD 40L、GATA3,NFAT단여병독재량불상관.결론 CD40L、BCL-10、GATA3、NFAT재만성을형간염환자PD-1/PD-L신호전도통로중가능유중요적조공작용,가이주심입적공능분석연구.
Objective To screen the gene expression profiles in PD-1/PD-L signal pathways in chronic hepatitis B( CHB )patients by gene expression microarray.Methods The differentially expressed genes in peripheral blood samples of 4 cases with CHB and 2 normal people was determined by Gene expression microarray,and then,in combination with preliminary construction of PD-1/PD-L signal pathways,selecting the consistent transcription factors or regulatory protein.The CD40L、BCL-I0、GATA3 and NFAT protein expression were detected by Western blot.Results 838 differentially expressed genes were detected,150 genes were upregulated and 688 genes were down-regulated.13 genes are belonging to the transcription factors or regulatory protein of PD-1/PD-L signaling pathways that we have constructed.In these 13 genes,6 genes (BCL-10、AP、SRY、MYOD1 、ELK4 、NFAT) are down-regulated and 7 genes( CD 40L、GADS、PDK1、IL-4 、GATA3 、CREB、RARG) are down-regulated.Using the Western blotting method,we found that BCL-10 protein expression was up- regulated in CHB patients,but CD 4 0 L,CATA 3 and NFAT proteins expression were down - regulated,which was not related with viral load carrying capacity.Conclusions CD 40L、BCL-10、GATA3 and NFAT.genes may have an important role in regulating the PD-1/PD-L signaling pathway.Further functional study is still need to be done.
