中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2010年
3期
155-158
,共4页
周桃梅%李金成%刘洪波%谭德明
週桃梅%李金成%劉洪波%譚德明
주도매%리금성%류홍파%담덕명
肝炎疫苗,乙型%HLA-D抗原%T淋巴细胞亚群%T淋巴细胞,辅助诱导%淋巴细胞活化%流式细胞术
肝炎疫苗,乙型%HLA-D抗原%T淋巴細胞亞群%T淋巴細胞,輔助誘導%淋巴細胞活化%流式細胞術
간염역묘,을형%HLA-D항원%T림파세포아군%T림파세포,보조유도%림파세포활화%류식세포술
Hepatitis B vaccines%HLA-D antigens%T-lymphocyte subsets%T-lymphocytes,helper-inducer%lymphocyte activation%Flow cytometry
目的 探讨乙型肝炎疫苗接种后免疫应答与T淋巴细胞亚群活化的关系.方法 采集18例乙型肝炎疫苗接种应答者、22例乙型肝炎疫苗接种无应答者、10例未接受乙型肝炎疫苗接种的健康对照者全血.用流式细胞术对其外周血单个核细胞的表面标志物CD69、CD4、CD8、CD3等进行检测.计算不同T淋巴细胞亚群百分比.并经t检验比较不同T淋巴细胞亚群CD69表达的差异.结果 在应答者中,外周血激活型T辅助淋巴细胞(CD69~+ CD4~+ T淋巴细胞)数明显升高,而CD69~+ CD8~+ T淋巴细胞数则明显减少.应答者、无应答者、对照者外周血CD69~+ CD4~+ T淋巴细胞分别占CD4~+ T淋巴细胞的(1.81±1.33)%、(0.64±0.48)%和(0.56±0.39)%,应答者分别与无应答者和对照者比较.差异均有统计学意义(t=3.444、3.940,均P<0.01).应答者、无应答者、对照者外周血CD69~+ CD8~+ T淋巴细胞分别占CD8~+ T淋巴细胞的(0.43±0.25)%、(3.05±1.69)%和(3.11±2.41)%,应答者分别与无应答者和对照者比较,差异均有统计学意义(t=7.997、5.944,均P<0.01).应答者、无应答者、对照者CD3~+ CD4~+ T淋巴细胞占CD3~+ T淋巴细胞的百分比和CD3~+ CD8~+ T淋巴细胞占CD3~+ T淋巴细胞的百分比比较差异均无统计学意义.结论 乙型肝炎疫苗接种后无应答与Th细胞早期活化不足有关.
目的 探討乙型肝炎疫苗接種後免疫應答與T淋巴細胞亞群活化的關繫.方法 採集18例乙型肝炎疫苗接種應答者、22例乙型肝炎疫苗接種無應答者、10例未接受乙型肝炎疫苗接種的健康對照者全血.用流式細胞術對其外週血單箇覈細胞的錶麵標誌物CD69、CD4、CD8、CD3等進行檢測.計算不同T淋巴細胞亞群百分比.併經t檢驗比較不同T淋巴細胞亞群CD69錶達的差異.結果 在應答者中,外週血激活型T輔助淋巴細胞(CD69~+ CD4~+ T淋巴細胞)數明顯升高,而CD69~+ CD8~+ T淋巴細胞數則明顯減少.應答者、無應答者、對照者外週血CD69~+ CD4~+ T淋巴細胞分彆佔CD4~+ T淋巴細胞的(1.81±1.33)%、(0.64±0.48)%和(0.56±0.39)%,應答者分彆與無應答者和對照者比較.差異均有統計學意義(t=3.444、3.940,均P<0.01).應答者、無應答者、對照者外週血CD69~+ CD8~+ T淋巴細胞分彆佔CD8~+ T淋巴細胞的(0.43±0.25)%、(3.05±1.69)%和(3.11±2.41)%,應答者分彆與無應答者和對照者比較,差異均有統計學意義(t=7.997、5.944,均P<0.01).應答者、無應答者、對照者CD3~+ CD4~+ T淋巴細胞佔CD3~+ T淋巴細胞的百分比和CD3~+ CD8~+ T淋巴細胞佔CD3~+ T淋巴細胞的百分比比較差異均無統計學意義.結論 乙型肝炎疫苗接種後無應答與Th細胞早期活化不足有關.
목적 탐토을형간염역묘접충후면역응답여T림파세포아군활화적관계.방법 채집18례을형간염역묘접충응답자、22례을형간염역묘접충무응답자、10례미접수을형간염역묘접충적건강대조자전혈.용류식세포술대기외주혈단개핵세포적표면표지물CD69、CD4、CD8、CD3등진행검측.계산불동T림파세포아군백분비.병경t검험비교불동T림파세포아군CD69표체적차이.결과 재응답자중,외주혈격활형T보조림파세포(CD69~+ CD4~+ T림파세포)수명현승고,이CD69~+ CD8~+ T림파세포수칙명현감소.응답자、무응답자、대조자외주혈CD69~+ CD4~+ T림파세포분별점CD4~+ T림파세포적(1.81±1.33)%、(0.64±0.48)%화(0.56±0.39)%,응답자분별여무응답자화대조자비교.차이균유통계학의의(t=3.444、3.940,균P<0.01).응답자、무응답자、대조자외주혈CD69~+ CD8~+ T림파세포분별점CD8~+ T림파세포적(0.43±0.25)%、(3.05±1.69)%화(3.11±2.41)%,응답자분별여무응답자화대조자비교,차이균유통계학의의(t=7.997、5.944,균P<0.01).응답자、무응답자、대조자CD3~+ CD4~+ T림파세포점CD3~+ T림파세포적백분비화CD3~+ CD8~+ T림파세포점CD3~+ T림파세포적백분비비교차이균무통계학의의.결론 을형간염역묘접충후무응답여Th세포조기활화불족유관.
Objective To investigate the relationship between early activation of T lymphocyte subsets and immune responses to hepatitis B surface antigen ( HBsAg) vaccination. Methods Peripheral blood samples were collected from 18 responders to HBsAg vaccination, 22 non-responders and 10 non-immunized healthy controls. Thereafter, the expressions of CD69, CD4, CD8, CD3 on peripheral blood mononuclear cell (PBMC) were determined by flow cytometry. The percentages of different T lymphocyte subsets were calculated. The expressions of CD69 on CD4~+ and CD8~+ lymphocytes were compared among the 3 groups and analyzed by t test. Results CD69~+ CD4~+ T lymphocytes increased and CD69~+ CD8~+ T lymphocytes decreased in the responders to HBsAg vaccination. The percentages of CD69~+ CD4~+ T lymphocytes in CD4~+ T lymphocytes of responders, non-responders and controls were (1.81 ± 1.33)%, (0. 64 ± 0.48)% and (0.56 ± 0. 39)%, respectively. CD69 expression on CD4~+ T lymphocytes in responders was increased as compared to non-responders as well as controls(t = 3. 444 and 3.940. respectively; both P < 0. 01). The percentage of CD69~+ CD8~+ T lymphocytes in CD8~+ T lymphocytes of responders was significantly lower than those of non-responders and controls [(0. 43±0. 25)% vs (3. 05± 1. 69)% vs (3.11± 2.41)%] (t = 7. 997 and 5. 944, respectively; both P<0. 01). The percentages of CD3~+ CD4~+ T lymphocytes and CD3~+ CD8~+ T lymphocytes in responders, non-responders and controls were not statistically different. Conclusion Failure of early activation of helper T cells may contribute to non response to HBsAg vaccination.
