中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
4期
744-746
,共3页
胡传义%刘修恒%葛名欢%宋超
鬍傳義%劉脩恆%葛名歡%宋超
호전의%류수항%갈명환%송초
前列腺癌%基因表达%肝细胞生长因子激活因子的抑制因子1
前列腺癌%基因錶達%肝細胞生長因子激活因子的抑製因子1
전렬선암%기인표체%간세포생장인자격활인자적억제인자1
Prostatic carcinoma%Gene expression%Hepatocyte growth factor activator inhibitor type 1
目的 探讨肝细胞生长因子激活因子的抑制因子(HAI-1)在前列腺癌(PCa)组织中的表达及其意义.方法 收集20例前列腺增生(BPH)和48例PCa患者的前列腺组织标本,应用逆转录-聚合酶链反应(RT-PCR)、蛋白印迹(Western blot)法分别从mRNA水平、蛋白水平检测HAI-1基因的表达.结果 HAI-1的阳性率在BPH和PCa中为85.00％、52.08％ (P ＜0.05)；HAI-1 mRNA的相对表达值分别为0.5482±0.0313、0.6718±0.0516(P ＜0.05)；HAI-1蛋白的相对表达值分别为0.5975±0.0518、0.7039±0.0377(P＜0.05)；随细胞分化程度的降低、临床分期的递增,HAI-1基因的表达量下降(P＜0.05),有淋巴结转移组显著低于无淋巴结转移组(P＜0.05).结论 HAI-1基因的表达缺失可作为前列腺癌浸润、转移和预后的一种分子标志.
目的 探討肝細胞生長因子激活因子的抑製因子(HAI-1)在前列腺癌(PCa)組織中的錶達及其意義.方法 收集20例前列腺增生(BPH)和48例PCa患者的前列腺組織標本,應用逆轉錄-聚閤酶鏈反應(RT-PCR)、蛋白印跡(Western blot)法分彆從mRNA水平、蛋白水平檢測HAI-1基因的錶達.結果 HAI-1的暘性率在BPH和PCa中為85.00％、52.08％ (P ＜0.05)；HAI-1 mRNA的相對錶達值分彆為0.5482±0.0313、0.6718±0.0516(P ＜0.05)；HAI-1蛋白的相對錶達值分彆為0.5975±0.0518、0.7039±0.0377(P＜0.05)；隨細胞分化程度的降低、臨床分期的遞增,HAI-1基因的錶達量下降(P＜0.05),有淋巴結轉移組顯著低于無淋巴結轉移組(P＜0.05).結論 HAI-1基因的錶達缺失可作為前列腺癌浸潤、轉移和預後的一種分子標誌.
목적 탐토간세포생장인자격활인자적억제인자(HAI-1)재전렬선암(PCa)조직중적표체급기의의.방법 수집20례전렬선증생(BPH)화48례PCa환자적전렬선조직표본,응용역전록-취합매련반응(RT-PCR)、단백인적(Western blot)법분별종mRNA수평、단백수평검측HAI-1기인적표체.결과 HAI-1적양성솔재BPH화PCa중위85.00％、52.08％ (P ＜0.05)；HAI-1 mRNA적상대표체치분별위0.5482±0.0313、0.6718±0.0516(P ＜0.05)；HAI-1단백적상대표체치분별위0.5975±0.0518、0.7039±0.0377(P＜0.05)；수세포분화정도적강저、림상분기적체증,HAI-1기인적표체량하강(P＜0.05),유림파결전이조현저저우무림파결전이조(P＜0.05).결론 HAI-1기인적표체결실가작위전렬선암침윤、전이화예후적일충분자표지.
Objective To investigate the expression and clinical significance of hepatocyte growth factor activator inhibitor type 1 ( HAI-1 ) gene in the tissues of prostatic carcinoma (PCa).Methods The prostate tissue specimens from 20 patients with benign prostate hyperplasia (BPH) and 48 patients with PCa were collected.Reverse transcription polymerase chain reaction ( RT-PCR ) and Western blotting were used to detect the expression of HAI-1 in these tissues.Results The positive expression rate of HAI-1 was 85.00％ and 52.08％ respectively in 20 cases of BPH and 48 cases of PCa tissues (P ＜0.05).The relative expression values of HAI-1 mRNA were 0.5482 ±0.0313 and 0.6718 ± 0.0516 respectively in PCa and BPH ( P ＜ 0.05 ).The relative expression values of HAI-1 protein in PCa and BPH tissues were 0.5975 ± 0.0518 and 0.7039 ± 0.0377 respectively (P ＜ 0.05 ).With the decrease in cellular differentiation and increase in the clinical staging,the expression of HAI-1 gene was decreased ( P ＜ 0.05 ).The expression of HAI-1 gene in lymph node metastasis group was significantly lower than that in non-lymph node metastasis group (P ＜ 0.05 ).Conclusion The HAI-1 gene deletion can be used as an important molecular marker in the invasion and metastasis of PCa.
