肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2011年
2期
97-99
,共3页
何艳姣%徐妙生%刘朝霞%俞进%李光
何豔姣%徐妙生%劉朝霞%俞進%李光
하염교%서묘생%류조하%유진%리광
乳腺肿瘤%β-catenin%突变
乳腺腫瘤%β-catenin%突變
유선종류%β-catenin%돌변
Breast neoplasms%β-catenin%Mutation
目的 探讨β-catenin基因在乳腺癌中的表达和突变情况.方法 应用免疫组织化学SP法检测119例乳腺癌、72例乳腺腺病和40例正常乳腺组织中β-catenin的表达,分析其与乳腺癌临床病理特征的关系.应用PCR和基因测序的方法检测44例乳腺癌β-catenin外显子3的突变情况.结果 40例正常乳腺组织中β-catenin在细胞膜中强表达.乳腺癌β-catenin异常表达率78.15%(93/119)明显高于乳腺腺病44.44%(32/72),组间比较差异有统计学意义(P＜0.01).β-catenin异常表达与淋巴结转移相关.44例乳腺癌组织中未检测到β-catenin基因外显子3的突变,β-catenin的异常表达率为77.30%(34/44).结论 β-catenin异常表达可作为评估乳腺癌转移预后、指导治疗的指标.乳腺癌组织中β-catenin异常表达不是由β-catenin基因突变所致,可能存在其他机制,有待于进一步研究.
目的 探討β-catenin基因在乳腺癌中的錶達和突變情況.方法 應用免疫組織化學SP法檢測119例乳腺癌、72例乳腺腺病和40例正常乳腺組織中β-catenin的錶達,分析其與乳腺癌臨床病理特徵的關繫.應用PCR和基因測序的方法檢測44例乳腺癌β-catenin外顯子3的突變情況.結果 40例正常乳腺組織中β-catenin在細胞膜中彊錶達.乳腺癌β-catenin異常錶達率78.15%(93/119)明顯高于乳腺腺病44.44%(32/72),組間比較差異有統計學意義(P＜0.01).β-catenin異常錶達與淋巴結轉移相關.44例乳腺癌組織中未檢測到β-catenin基因外顯子3的突變,β-catenin的異常錶達率為77.30%(34/44).結論 β-catenin異常錶達可作為評估乳腺癌轉移預後、指導治療的指標.乳腺癌組織中β-catenin異常錶達不是由β-catenin基因突變所緻,可能存在其他機製,有待于進一步研究.
목적 탐토β-catenin기인재유선암중적표체화돌변정황.방법 응용면역조직화학SP법검측119례유선암、72례유선선병화40례정상유선조직중β-catenin적표체,분석기여유선암림상병리특정적관계.응용PCR화기인측서적방법검측44례유선암β-catenin외현자3적돌변정황.결과 40례정상유선조직중β-catenin재세포막중강표체.유선암β-catenin이상표체솔78.15%(93/119)명현고우유선선병44.44%(32/72),조간비교차이유통계학의의(P＜0.01).β-catenin이상표체여림파결전이상관.44례유선암조직중미검측도β-catenin기인외현자3적돌변,β-catenin적이상표체솔위77.30%(34/44).결론 β-catenin이상표체가작위평고유선암전이예후、지도치료적지표.유선암조직중β-catenin이상표체불시유β-catenin기인돌변소치,가능존재기타궤제,유대우진일보연구.
Objective To discuss the expression and mutation of β-catenin gene in breast carcinoma.Methods 119 breast carcinomas, 72 adenosis of breast tissues and 40 normal breast tissues were studied.Using immunohistochemistry studies to detect the expressions of β-catenin, then analyzing their relationship with the clinicopathological features of breast carcinomas. Gene mutation of β-catenin (exon 3) in 44 cases of breast carcinoma were analyzed by polymerase chain reaction (PCR) and then by direct sequencing.Results In 40 cases of normal breast tissues, epithelial cells showed equally strong membranous of β-catenin protein at the cell-cell boundaries. The abnormal rate of β-catenin in breast carcinomas (78.15 %) was much higher than that of adenosis of breast tissues (44.44 %), the significant correlation was found (P＜0.01). The abnormal expression of β-catenin was associated with lymph node metastasis (P=0.031). Mutation of β-catenin gene was not detected in 44 cases of breast carcinoma, while the abnormal rate of β-catenin in 44 cases of breast carcinoma was 77.30 %. Conclusion β-catenin abnormal expression could be considered as an useful marker for determining metastasis and prognosis of human breast carcinoma and for guiding treatment. β-catenin abnormal expression is not caused by β-catenin gene mutation, maybe by other mechanisms. It needs further study.
