中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
9期
602-605
,共4页
张功员%陈萍萍%李晟磊%尹磊%高冬玲%乐晓萍%陈奎生%张云汉%张钦宪
張功員%陳萍萍%李晟磊%尹磊%高鼕玲%樂曉萍%陳奎生%張雲漢%張欽憲
장공원%진평평%리성뢰%윤뢰%고동령%악효평%진규생%장운한%장흠헌
食管肿瘤%鳞状细胞%干细胞因子
食管腫瘤%鱗狀細胞%榦細胞因子
식관종류%린상세포%간세포인자
Esophageal neoplasm%Squamous cell%Nucleostemin
目的 探讨食管鳞状细胞癌组织中核干细胞因子(NS)基因mRNA的表达水平.方法 应用实时定量PCR检测62例食管鳞状细胞癌组织及其配对正常食管黏膜组织(62例)中NS基因mRNA表达水平,分析核干细胞因子mRNA表达水平与食管鳞状细胞癌患者临床病理参数间的关系.结果 62例食管鳞状细胞癌组织中NS基因mRNA表达水平(4.5 ±2.1)高于其配对正常食管黏膜组织(2.1±1.3)表达水平(t=-5.045,P=0.000).NS基因mRNA表达水平与病理分级、浸润深度和淋巴结转移等临床参数有关(均P＜0.05),与性别、年龄、病理类型等参数无关(均P＞0.05).多元线型回归分析显示,临床病理参数在NS基因mRNA表达中起重要作用(P=0.000),NS基因mRNA的表达主要受浸润深度和淋巴结转移的影响(P＜0.05).结论 食管鳞状细胞癌的发生发展和增殖过程中NS基因mRNA起重要作用.
目的 探討食管鱗狀細胞癌組織中覈榦細胞因子(NS)基因mRNA的錶達水平.方法 應用實時定量PCR檢測62例食管鱗狀細胞癌組織及其配對正常食管黏膜組織(62例)中NS基因mRNA錶達水平,分析覈榦細胞因子mRNA錶達水平與食管鱗狀細胞癌患者臨床病理參數間的關繫.結果 62例食管鱗狀細胞癌組織中NS基因mRNA錶達水平(4.5 ±2.1)高于其配對正常食管黏膜組織(2.1±1.3)錶達水平(t=-5.045,P=0.000).NS基因mRNA錶達水平與病理分級、浸潤深度和淋巴結轉移等臨床參數有關(均P＜0.05),與性彆、年齡、病理類型等參數無關(均P＞0.05).多元線型迴歸分析顯示,臨床病理參數在NS基因mRNA錶達中起重要作用(P=0.000),NS基因mRNA的錶達主要受浸潤深度和淋巴結轉移的影響(P＜0.05).結論 食管鱗狀細胞癌的髮生髮展和增殖過程中NS基因mRNA起重要作用.
목적 탐토식관린상세포암조직중핵간세포인자(NS)기인mRNA적표체수평.방법 응용실시정량PCR검측62례식관린상세포암조직급기배대정상식관점막조직(62례)중NS기인mRNA표체수평,분석핵간세포인자mRNA표체수평여식관린상세포암환자림상병리삼수간적관계.결과 62례식관린상세포암조직중NS기인mRNA표체수평(4.5 ±2.1)고우기배대정상식관점막조직(2.1±1.3)표체수평(t=-5.045,P=0.000).NS기인mRNA표체수평여병리분급、침윤심도화림파결전이등림상삼수유관(균P＜0.05),여성별、년령、병리류형등삼수무관(균P＞0.05).다원선형회귀분석현시,림상병리삼수재NS기인mRNA표체중기중요작용(P=0.000),NS기인mRNA적표체주요수침윤심도화림파결전이적영향(P＜0.05).결론 식관린상세포암적발생발전화증식과정중NS기인mRNA기중요작용.
Objective To investigate the mRNA expression levels of nucleostemin(NS)in human esophageal squainous cell carcinoma tissue.Methods Real-time PCR was used to quantify the mRNA expression of NS in the samples of esophageal squamous eell carcinoma tissue and their matched normal esophageal mucosa tissue from 62 patients,36 males and 26 females,aged(61±10)(38-75).The relationship between NS mRNA expression level and clinical pathological features was analyzed.Results The NS mRNA expression level of the 62 cases of esophageal squamous cell carcinoma tissue was ( 4.5±2.1),significantly higher than that of the matched normal esophageal mucosa tissue[(2.1±1.3),t=-5.045,P=0.000 ].The mRNA expression level of NS was associated with tumor grade,depth of infiltration,and lymph node metastasis(all P＜0.05),but not with gender,age,and pathological type(all P＞0.05).Multiple linear regression analysis revealed that clinical and pathological features influenced the NS mRNA expression level(P=0.000),and the depth of infiltration and lymph node metastasis were important influencing factors for NS mRNA expression level(both P＜0.05).Conclusion NS may play an important role in the progression and proliferation of esophageal squamous cell carcinoma.
