CAJ | 학술논문

电子克隆提供了一种利用基因组数据库克隆新基因全长cDNA序列的策略.利用小鼠Irak-1基因编码序列(NM_008363)为种子序列进行电子克隆获得了牛Irak-1基因完整编码序列.然后,用生物信息学方法分析了该基因的结构,微卫星位点,密码子偏性和氨基酸的同源性等.结果表明:该基因cDNA全长2 645bp,无内含子,最大开放阅读框2 157bp,编码718个氨基酸,与小鼠的同源性为77%.
전자극륭제공료일충이용기인조수거고극륭신기인전장cDNA서렬적책략.이용소서Irak-1기인편마서렬(NM_008363)위충자서렬진행전자극륭획득료우Irak-1기인완정편마서렬.연후,용생물신식학방법분석료해기인적결구,미위성위점,밀마자편성화안기산적동원성등.결과표명:해기인cDNA전장2 645bp,무내함자,최대개방열독광2 157bp,편마718개안기산,여소서적동원성위77%.
In silico cloning provided a strategy of gene cloning that can obtain the full-length cDNA sequence of a novel gene by using genebank. The complete coding sequence of cow Irak-1 gene was obtained by using mouse Irak-1 gene sequence (NM_008363) to be probe in silico cloning. Then the structure of Irak-1 gene,ssr,codon preference,and the constitution of amino acid of IRAK-1 protein in cow are analyzed by using bioinformatics methods. The results show that the cDNA was 2 645bp,with no intron,contains a 2 157bp open reading frame,encoding 718aa protein. The cow Irak-1 gene showed 77% similarity to corresponding mouse respectively.