中华耳科学杂志
中華耳科學雜誌
중화이과학잡지
CHINESE JOURNAL OF OTOLOGY
2009年
4期
352-356
,共5页
陈伟%郭维维%胡吟燕%孙建和%于宁%韩东一%翟所强%杨伟炎%何志洲%杨仕明
陳偉%郭維維%鬍吟燕%孫建和%于寧%韓東一%翟所彊%楊偉炎%何誌洲%楊仕明
진위%곽유유%호음연%손건화%우저%한동일%적소강%양위염%하지주%양사명
基因转导%噪声性聋%Math1%豚鼠%ABR
基因轉導%譟聲性聾%Math1%豚鼠%ABR
기인전도%조성성롱%Math1%돈서%ABR
Gene transferring%Hath1%NIHL%Guinea pig%ABR
目的 观察Math1基因内耳导入对噪声性聋豚鼠听功能的影响,探讨Math1基因过表达对噪声损伤耳蜗的生物学效应,为内耳基因治疗提供实验基础和理论依据.方法 经脉冲噪声致聋的豚鼠45只(各频率ABR阈值均≥95 dB SPL),雌雄不限,实验开始时体重250～300g.随机分为3组:Ad-Math1-EGFP组(30只);AdEGFP组(5只);空白组(10只).各组豚鼠在基因转导后4周、8周分别测试双耳ABR.测试完毕后处死动物,观察听泡及耳蜗无炎性病变者记录听阈结果 .结果 Math1导入后4周,导入耳各频率ABR阈值低于对照耳(右耳),也低于Ad-EGFP组及空白组,平均达到85 dB SPL.Math1导入后8周,导入耳各频率ABR阈值低于对照耳(右耳),也低于Ad-EGFP组及空白组,与4周时比较,进一步好转,平均达到75 dB SPL.结论 Math1基因内耳导入可使噪声导致全聋的豚鼠听功能部分恢复,为噪声性聋的治疗打开了新的思路和手段.
目的 觀察Math1基因內耳導入對譟聲性聾豚鼠聽功能的影響,探討Math1基因過錶達對譟聲損傷耳蝸的生物學效應,為內耳基因治療提供實驗基礎和理論依據.方法 經脈遲譟聲緻聾的豚鼠45隻(各頻率ABR閾值均≥95 dB SPL),雌雄不限,實驗開始時體重250～300g.隨機分為3組:Ad-Math1-EGFP組(30隻);AdEGFP組(5隻);空白組(10隻).各組豚鼠在基因轉導後4週、8週分彆測試雙耳ABR.測試完畢後處死動物,觀察聽泡及耳蝸無炎性病變者記錄聽閾結果 .結果 Math1導入後4週,導入耳各頻率ABR閾值低于對照耳(右耳),也低于Ad-EGFP組及空白組,平均達到85 dB SPL.Math1導入後8週,導入耳各頻率ABR閾值低于對照耳(右耳),也低于Ad-EGFP組及空白組,與4週時比較,進一步好轉,平均達到75 dB SPL.結論 Math1基因內耳導入可使譟聲導緻全聾的豚鼠聽功能部分恢複,為譟聲性聾的治療打開瞭新的思路和手段.
목적 관찰Math1기인내이도입대조성성롱돈서은공능적영향,탐토Math1기인과표체대조성손상이와적생물학효응,위내이기인치료제공실험기출화이론의거.방법 경맥충조성치롱적돈서45지(각빈솔ABR역치균≥95 dB SPL),자웅불한,실험개시시체중250～300g.수궤분위3조:Ad-Math1-EGFP조(30지);AdEGFP조(5지);공백조(10지).각조돈서재기인전도후4주、8주분별측시쌍이ABR.측시완필후처사동물,관찰은포급이와무염성병변자기록은역결과 .결과 Math1도입후4주,도입이각빈솔ABR역치저우대조이(우이),야저우Ad-EGFP조급공백조,평균체도85 dB SPL.Math1도입후8주,도입이각빈솔ABR역치저우대조이(우이),야저우Ad-EGFP조급공백조,여4주시비교,진일보호전,평균체도75 dB SPL.결론 Math1기인내이도입가사조성도치전롱적돈서은공능부분회복,위조성성롱적치료타개료신적사로화수단.
Objective To evaluate the effect of adenovirus mediated Mathl gene transferring into the cochlea of noise-induced hearing loss (NIHL) guinea pig to assess its influence on hearing and to provide experiment foundation for inner ear gene therapy.Methods Forty-five NIHL guinea pigs (audibility threshold≥95 dB SPL),with no sex limited,weight between 250 and 300 g before this experiment,were divided into three groups randomly.Thirty guinea pigs (AdMath1-EGFP group) were implanted with adenovirus mediated Hath1 gene.Five guinea pigs (Ad-EGFP group) were implanted with enhanced green fluorescent protein (EGFP) and ten without treatment as blank group.Auditory brainstem response (ABR) thresholds were determined in all animals at 4 weeks and 8 weeks after surgery.Results The ABR threshold of the ear inoculated in the Ad-Math1-EGFP group was lower than the control ear (right ear) and the Ad-EGFP group and the blank group 4 weeks after surgery (85 dB SPL in average).The ABR threshold of the ear inoculated in the Ad-Math1-EGFP group was lower than the control ear (right ear) and the Ad-EGFP group and the blank group 8 weeks after surgery,and better than that 4 weeks before (75 dB SPL in average).Conclusion Math1 gene transferred into the cochlea can partially recover the hearing loss which provides a new method for NIHL therapy.
