CAJ | 학술논문

[目的]研究化学转化过程中热激后冰浴时间和复苏时间对转化效率的影响,确定简便快速的转化方法.[方法]采用2种缓冲溶液制备感受态,以热激后冰浴时间或复苏时间为变量,研究这2个因素与转化效率的关系.[结果]冰浴2 min,复苏30或40 min达到最佳转化效率;冰浴和复苏时间为0时仍能获得相当数量的转化子.[结论]热激后冰浴和复苏时间对转化效率有一定影响,但不是决定性因素.因此,在一般性转化试验中可以省略这2个步骤以节省时间.
[목적]연구화학전화과정중열격후빙욕시간화복소시간대전화효솔적영향,학정간편쾌속적전화방법.[방법]채용2충완충용액제비감수태,이열격후빙욕시간혹복소시간위변량,연구저2개인소여전화효솔적관계.[결과]빙욕2 min,복소30혹40 min체도최가전화효솔;빙욕화복소시간위0시잉능획득상당수량적전화자.[결론]열격후빙욕화복소시간대전화효솔유일정영향,단불시결정성인소.인차,재일반성전화시험중가이성략저2개보취이절성시간.
[Objective] The aim of the research was to study the effect of the ice bath time after heat shock and the incubation time on the transformation efficacy, and to establish a simple and quick transformation method. [Method] Competent cells were prepared with two buffer solutions; with the ice bath time after heat shock and the recovery time as the variables, the relationship between these two factors and transformation efficacy was studied. [Result] The transformation efficacy was the best when the ice bath time was 2 min and the recovery time was 30 or 40 min; when the ice bath time and the recovery time was 0 min, a certain amount of transformants still could be obtained. [Conclusion] The ice bath time after heat shock and the recovery time had certain impact on transformation efficacy, but they were not the decisive factors. Therefore, in the general transformation experiment, these two steps could be omitted.