中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
1期
26-29
,共4页
胰腺癌%RUNX3基因%Cyclin D1基因
胰腺癌%RUNX3基因%Cyclin D1基因
이선암%RUNX3기인%Cyclin D1기인
Pancreatic carcinoma%Runt-related transcription factor 3 gene%Cyclin D1 gene
目的 探讨人RUNT相关转录因子3(RUNX3)、细胞周期素(Cyclin D1)基因在胰腺癌组织和癌旁组织中mRNA表达水平与临床意义.方法 采用逆转录-聚合酶链反应(RT-PCR)检测RUNX3、Cyclin D1基因在42例胰腺癌组织及其癌旁组织中mRNA表达水平,并分析RUNX3、Cyclin D1基因mRNA表达与临床病理因素的关系.结果 (1)RUNX3基因在42例胰腺癌组织中mRNA表达量相对值为:0.2469±0.0708；相应癌旁组织中相对值为:0.7091±0.1764,两者差异有统计学意义(t=15.7036,P＜0.01).(2) RUNX3基因mRNA表达水平在分化程度、淋巴结转移、临床分期等病理因素的差异有统计学意义(P＜0.05),而与性别、年龄等因素无明显相关(P＞0.05).(3) Cyclin D1基因在42例胰腺癌组织中mRNA表达量相对值为:0.7769±0.1456；相应癌旁组织中相对值为:0.2860±0.1224,两者差异有统计学意义(t=18.9158,P＜0.01).(4) Cyclin D1基因mRNA表达水平在分化程度、淋巴结转移、临床分期等病理因素的差异有统计学意义(P＜0.05),而与性别、年龄等因素无明显相关(P＞0.05).(5)胰腺癌中RUNX3基因的mRNA表达抑制与Cyclin D1基因的过表达呈明显相关(r=-0.320,P＜0.05).结论 RUNX3基因在胰腺癌组织中mRNA 表达抑制,Cyclin D1基因在胰腺癌组织中mRNA过表达,RUNX3表达抑制或缺失及Cyclin D1过表达与胰腺癌的发生、发展有关.
目的 探討人RUNT相關轉錄因子3(RUNX3)、細胞週期素(Cyclin D1)基因在胰腺癌組織和癌徬組織中mRNA錶達水平與臨床意義.方法 採用逆轉錄-聚閤酶鏈反應(RT-PCR)檢測RUNX3、Cyclin D1基因在42例胰腺癌組織及其癌徬組織中mRNA錶達水平,併分析RUNX3、Cyclin D1基因mRNA錶達與臨床病理因素的關繫.結果 (1)RUNX3基因在42例胰腺癌組織中mRNA錶達量相對值為:0.2469±0.0708；相應癌徬組織中相對值為:0.7091±0.1764,兩者差異有統計學意義(t=15.7036,P＜0.01).(2) RUNX3基因mRNA錶達水平在分化程度、淋巴結轉移、臨床分期等病理因素的差異有統計學意義(P＜0.05),而與性彆、年齡等因素無明顯相關(P＞0.05).(3) Cyclin D1基因在42例胰腺癌組織中mRNA錶達量相對值為:0.7769±0.1456；相應癌徬組織中相對值為:0.2860±0.1224,兩者差異有統計學意義(t=18.9158,P＜0.01).(4) Cyclin D1基因mRNA錶達水平在分化程度、淋巴結轉移、臨床分期等病理因素的差異有統計學意義(P＜0.05),而與性彆、年齡等因素無明顯相關(P＞0.05).(5)胰腺癌中RUNX3基因的mRNA錶達抑製與Cyclin D1基因的過錶達呈明顯相關(r=-0.320,P＜0.05).結論 RUNX3基因在胰腺癌組織中mRNA 錶達抑製,Cyclin D1基因在胰腺癌組織中mRNA過錶達,RUNX3錶達抑製或缺失及Cyclin D1過錶達與胰腺癌的髮生、髮展有關.
목적 탐토인RUNT상관전록인자3(RUNX3)、세포주기소(Cyclin D1)기인재이선암조직화암방조직중mRNA표체수평여림상의의.방법 채용역전록-취합매련반응(RT-PCR)검측RUNX3、Cyclin D1기인재42례이선암조직급기암방조직중mRNA표체수평,병분석RUNX3、Cyclin D1기인mRNA표체여림상병리인소적관계.결과 (1)RUNX3기인재42례이선암조직중mRNA표체량상대치위:0.2469±0.0708；상응암방조직중상대치위:0.7091±0.1764,량자차이유통계학의의(t=15.7036,P＜0.01).(2) RUNX3기인mRNA표체수평재분화정도、림파결전이、림상분기등병리인소적차이유통계학의의(P＜0.05),이여성별、년령등인소무명현상관(P＞0.05).(3) Cyclin D1기인재42례이선암조직중mRNA표체량상대치위:0.7769±0.1456；상응암방조직중상대치위:0.2860±0.1224,량자차이유통계학의의(t=18.9158,P＜0.01).(4) Cyclin D1기인mRNA표체수평재분화정도、림파결전이、림상분기등병리인소적차이유통계학의의(P＜0.05),이여성별、년령등인소무명현상관(P＞0.05).(5)이선암중RUNX3기인적mRNA표체억제여Cyclin D1기인적과표체정명현상관(r=-0.320,P＜0.05).결론 RUNX3기인재이선암조직중mRNA 표체억제,Cyclin D1기인재이선암조직중mRNA과표체,RUNX3표체억제혹결실급Cyclin D1과표체여이선암적발생、발전유관.
Objective To study the expression of human runt-related transcription factor 3 (RUNX3) and Cyclin D1 mRNA in pancreatic carcinoma and their correlation with the clinicopathologic features.Methods The expression levels of RUNX3 and Cyclin D1 mRNA were detected by using reverse transcription polymerase chain reaction (RT-PCR) in 42 cases of pancreatic carcinoma tissues and their surrounding normal tissues.Results ( 1 ) The relative expression level of RUNX3 mRNA in 42 cases of pancreatic carcinoma tissues was 0.2469 ±0.0708,and 0.7091 ±0.1764 in 42 cases of surrounding normal tissues.There was significant difference in the RUNX3 expression between two kinds of samples (t =15.7036,P ＜ 0.01 ).The expression of RUNX3 mRNA in pancreatic carcinoma was correlated to histologic grade with clinical staging and lymph node metastasis ( P ＜0.05 ),but not to age and sex; (2) The relative expression level of Cyclin D1 mRNA in 42 cases of pancreatic carcinoma tissues was 0.7769 ±0.1456,and 0.2860 ± 0.1224 in 42 cases of surrounding normal tissue.There was significnat difference in the Cyclin D 1 expression between two kinds of samples ( t =18.9158,P ＜ 0.01 ).The expression of Cyclin D1 mRNA in pancreatic carcinoma was correlated to histologic grade with clinical staging and lymph node metastasis (P ＜ 0.05 ),but not to age and sex; (3) The expression of RUNX3 was correlated with Cyclin D1 (r =-0.320,P ＜0.05).Conclusion The expression of RUNX3 mRNA was weak and that of Cyclin D1 mRNA was high in pancreatic carcinoma.They may play an important role in the carcinogenesis and progression of pancreatic carcinoma.
