CAJ | 학술논문

目的研究中药复方平肺口服液对放射性肺炎大鼠肺组织肥大细胞的分布和细胞因子白介素-6(IL-6)表达的影响.方法取SD大鼠45只,随机分为3组,即正常对照组,模型组和平肺口服液组.全胸单次照射20 Gy建立大鼠放射性肺纤维化模型,于照射前1周开始灌服平肺口服液,20 g·kg-1·d-1,共5周.分别于照射后2、4和8周用甲苯胺蓝染色法,观察肥大细胞在肺组织中的分布,用免疫组化法观察照射后8周肺组织IL-6蛋白的表达,用RT-PCR方法观察照射后4周肺组织IL-6基因的表达.结果大鼠全胸单次照射20 Gy后8周肺组织可见大量肥大细胞聚集(P<0.01),IL-6蛋白表达明显增加(P<0.01);照射后4周肺组织IL-6基因表达明显增高(P<0.01).中药复方平肺口服液可明显减少肥大细胞在肺组织的聚集(P<0.01)降低IL-6蛋白(P<0.01)和基因的表达(P<0.05).结论中药复方平肺口服液能够通过在肺组织抑制肥大细胞的聚集和IL-6的合成进而预防大鼠放射性肺炎的发生.
목적 연구중약복방평폐구복액대방사성폐염대서폐조직비대세포적분포화세포인자백개소-6(IL-6)표체적영향.방법 취SD대서45지,수궤분위3조,즉정상대조조,모형조화평폐구복액조.전흉단차조사20 Gy건립대서방사성폐섬유화모형,우조사전1주개시관복평폐구복액,20 g·kg-1·d-1,공5주.분별우조사후2、4화8주용갑분알람염색법,관찰비대세포재폐조직중적분포,용면역조화법관찰조사후8주폐조직IL-6단백적표체,용RT-PCR방법관찰조사후4주폐조직IL-6기인적표체.결과 대서전흉단차조사20 Gy후8주폐조직가견대량비대세포취집(P<0.01),IL-6단백표체명현증가(P<0.01);조사후4주폐조직IL-6기인표체명현증고(P<0.01).중약복방평폐구복액가명현감소비대세포재폐조직적취집(P<0.01)강저IL-6단백(P<0.01)화기인적표체(P<0.05).결론 중약복방평폐구복액능구통과재폐조직억제비대세포적취집화IL-6적합성진이예방대서방사성폐염적발생.
Objective To investigate the effect of Pingfei Oral Liquid (POL) on the distribution of mast cells (MCs) and the expression of interleukin 6 (IL-6) in the lung tissue of radiation pneumonia rats. Methods Forty-five SD rats were randomized into 3 groups : normal control, model group and POL group. The rat model of radiation lung fibro-sis was set up by a single X-ray dose of 20Gy irradiation over the whole chest of the rats. POL (20 g·kg-1·d-1,once a day, five times a week) was given orally one week before irradiation and the treatment lasted 5 weeks. MCs in the lung tissue were stained with toluidine blue firstly and then were counted 2, 4 and 8 weeks after irradiation. IL-6 protein expression of lung tissue was measured by immunohistochemical assay 8 weeks after irradiation, and mRNA ex-pression was determined with RT-PCR 4 weeks after irradiation. Results It's showed the aggregation of large amount of pulmonary mast cells and increase of IL-6 protein expression 8 weeks after irradiation (P < 0.01). IL-6 mRNA expression in the irradiated lung of rats increased 4 weeks after irradiation (P < 0. 01). POL could reduce the aggrega- tion of MCs (P < 0. 01) and the expression of IL-6 protein (P < 0. 01) and mRNA (P < 0. 05) in the lung tissue. Conclusion POL can prevent radiation pneumonia in rats by reducing the aggregation of mast cells and inhibiting IL-6 expression in the lung tissue.